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Imperial College London/Notebook/25 September 2009
From 2009.igem.org
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| - | + | ==Testing team== | |
| - | == | + | ===OD calibration=== |
| + | *Carried out 10<sup>-4</sup> and 10<sup>-6</sup> dilutions | ||
| + | *Carried out on ice | ||
| + | *Used 3 OD values: 1, 0.7 and 0.4 | ||
| + | *Conclusions: | ||
| + | **Too many colonies on 10<sup>-4</sup>, too few on 10<sup>-6</sup>, 10<sup>-5</sup> seems to be the most appropriate | ||
| + | *Suggestions: | ||
| + | **Dilute using factors: 10<sup>-4</sup>, 10<sup>-5</sup> and 10<sup>-6</sup> with 100uL inoculation | ||
| + | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 15:16, 1 October 2009
Testing team
OD calibration
- Carried out 10-4 and 10-6 dilutions
- Carried out on ice
- Used 3 OD values: 1, 0.7 and 0.4
- Conclusions:
- Too many colonies on 10-4, too few on 10-6, 10-5 seems to be the most appropriate
- Suggestions:
- Dilute using factors: 10-4, 10-5 and 10-6 with 100uL inoculation
