Team:Imperial College London/Wetlab/Protocols/Autoinduction

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! Assay
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! Overview and Aims
! Overview and Aims
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| <b>Promoter Characterisation</b>
| <b>Promoter Characterisation</b>
| [[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]]
| [[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]]
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* To determine the best secondary carbon source for optimal growth
* To determine the best secondary carbon source for optimal growth
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| <b>Auto-Induction</b>
| <b>Auto-Induction</b>
| [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|Glucose Time Delay]]
| [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|Glucose Time Delay]]
| * Characterise the tunable time duration it takes before GFP expression (M2 activation)
| * Characterise the tunable time duration it takes before GFP expression (M2 activation)
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==Glucose time delay==
 
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===Aims===
 
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* Characterise the tunable time duration it takes before GFP expression (M2 activation)
 
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===Assay===
 
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*The cells will be grown until OD= 0.7. 
 
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*The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
 
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*OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].
 
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*The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
 
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*The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)
 
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[[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|See Glucose time delay for details]]
 
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Latest revision as of 15:01, 23 September 2009



Section Assay Overview and Aims
Promoter Characterisation Secondary Carbon Source Diauxie Growth
  • To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling
  • To determine the best secondary carbon source for optimal growth
Auto-Induction Glucose Time Delay * Characterise the tunable time duration it takes before GFP expression (M2 activation)

Mr. Gene   Geneart   Clontech   Giant Microbes

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