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Team:Imperial College London/Wetlab/Protocols/Autoinduction
From 2009.igem.org
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{{Imperial/09/TemplateTop}} | {{Imperial/09/TemplateTop}} | ||
{{Imperial/09/Tabs/Main/Wetlab/Protocols}} | {{Imperial/09/Tabs/Main/Wetlab/Protocols}} | ||
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{| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" | {| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" | ||
! Section | ! Section | ||
! Assay | ! Assay | ||
! Overview and Aims | ! Overview and Aims | ||
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>Promoter Characterisation</b> | | <b>Promoter Characterisation</b> | ||
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* To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling | * To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling | ||
* To determine the best secondary carbon source for optimal growth | * To determine the best secondary carbon source for optimal growth | ||
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>Auto-Induction</b> | | <b>Auto-Induction</b> | ||
Latest revision as of 15:01, 23 September 2009
| Section | Assay | Overview and Aims |
|---|---|---|
| Promoter Characterisation | Secondary Carbon Source Diauxie Growth |
|
| Auto-Induction | Glucose Time Delay | * Characterise the tunable time duration it takes before GFP expression (M2 activation) |
