"
Team:Chiba/Notebook/Calendar/9 September 2009
From 2009.igem.org
(Difference between revisions)
(→Lab Notebook) |
(→Lab Notebook) |
||
| (5 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
([[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/10_September_2009|10_September_2009]]) | ([[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/10_September_2009|10_September_2009]]) | ||
| - | + | == Check of Sender's AHL generation (1) == | |
| - | + | === 08:00 main culture === | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | === | + | |
:*divided injection 12.5ml of the culture to 10 50ml centrifuging tubes | :*divided injection 12.5ml of the culture to 10 50ml centrifuging tubes | ||
:*WASH 3 times | :*WASH 3 times | ||
| Line 19: | Line 15: | ||
:*make 10 concentration varieties of solid media | :*make 10 concentration varieties of solid media | ||
| - | + | == Handwritten Lab Notebook == | |
| - | == Lab Notebook == | + | |
[[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]] | [[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]] | ||
Latest revision as of 18:25, 23 September 2009
(8_September_2009 <|>10_September_2009)
Check of Sender's AHL generation (1)
08:00 main culture
- divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
- WASH 3 times
- Add 12.5ml new culture
- Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
- Do same operation every 15min to 2nd~10th tubes
- after all, mix the supernatant and solid media at 1:1
- make 10 concentration varieties of solid media
Handwritten Lab Notebook
