Team:Utah State/Project

From 2009.igem.org

(Difference between revisions)
(→Overall project)
 
(61 intermediate revisions not shown)
Line 1: Line 1:
-
{|style="font color="#CC3300"; background-color:#212223; cellpadding="3" cellspacing="5" border="2" bordercolor="#cd0000"border-spacing:6px; text-align:center" width="960px"
+
<!--- BANNER--->
-
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State|<font color="#ffffff">Home</font>]]
+
-
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Team|<font color="#ffffff">The Team</font>]]
+
-
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Project|<font color="#A9A9A9">The Project</font>]]
+
-
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Parts|<font color="#ffffff">Parts</font>]]
+
-
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Notebook|<font color="#ffffff">Notebook</font>]]
+
-
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Protocols|<font color="#ffffff">Protocols</font>]]
+
-
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Links|<font color="#ffffff">Links</font>]]
+
-
|}
+
-
 
+
-
<!--- The Mission, Experiments --->
+
<html>
<html>
-
<style type="text/css">
+
<a href="https://2009.igem.org/Team:Utah_State">
-
body {background-image:url("https://static.igem.org/mediawiki/2008/6/63/Bkgnd14.gif");background-repeat:repeat; }
+
<img alt="USU iGem" src="https://static.igem.org/mediawiki/2009/7/71/USUlogo.jpg"/> </a>
-
</style>  
+
<!--- Table--->
-
</html>
+
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<p class="class"><title>Untitled Document</title>
 +
</head>
 +
<style type="text/css">
 +
#ana {
 +
width: 11em;
 +
display : block;
 +
color : #999;
 +
p class="class"> font-weight : none;
 +
font-family:Century Gothic, Arial, sans-serif;
 +
text-decoration : none;
 +
background-color : #231f20;
 +
 +
}
-
{|align="justify"
+
#ana .currentPage {
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
padding-left:9px;
-
|[[Image:Example_logo.png|200px|right]]
+
        border-top-style: none;
-
|-
+
color : #56b8df;
-
|
+
width: 6em;
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
display : block;
-
|
+
font-weight : none;
-
|-
+
font-family:Century Gothic, Arial, sans-serif;
-
|
+
text-decoration : none;
-
|align="center"|[[Team:Utah_State | Team Example 2]]
+
-
|}
+
}
 +
 +
#ana a {
 +
font-size:x-small;
 +
text-decoration : none;
 +
padding-left:17px;
 +
color : #E0FFFF;
 +
font-family: Verdana, Arial, sans-serif;
 +
             
 +
               
 +
}
 +
#ana a:hover{
 +
 +
text-decoration:underline;
 +
 +
}
 +
#nav a {
 +
padding-left: 9px;
 +
width:10em;
 +
display : block;
 +
color : #999;
 +
font-weight : none;
 +
font-family:Century Gothic, Arial, sans-serif;
 +
        font size="3";
 +
text-decoration : none;
 +
        text-align : left;
 +
background-color : #231f20;
 +
}
-
== '''Overall project''' ==
+
#nav a:hover {
 +
visibility:visible;
 +
              background-color : #474e54;
 +
 +
color : #56b8df;}
-
'''Bacteria without Borders:
+
</style>
-
Investigating a multi-host BioBrick vector and secretion of cellular products
+
<body bgcolor="#231f20">
-
'''
+
<table width="100%" border="0">
 +
  <tr>
 +
    <td width="16%" valign="top"><table width="72%" border="0">
 +
      <tr valign="top">
 +
        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State"><font size = 4>HOME</font></a></td>
 +
      </tr>
 +
      <tr>
 +
        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Team"><font size = 4>ABOUT US</font></a></td>
 +
      </tr>
 +
      <tr>
 +
        <td width="172" id="ana"><span class="currentPage"><font size = 4>PROJECT</font></span>
-
The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.
+
        <a href="https://2009.igem.org/Team:Utah_State/Abstract">Abstract</a><br />
 +
<a href="https://2009.igem.org/Team:Utah_State/Introduction">Introduction</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/Broad-HostVectors">Broad-Host Vectors</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/Secretion">Secretion</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/Experiments">Experiments</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/FutureWork">Future Work</a><br />
 +
        <a href="https://2009.igem.org/Team:Utah_State/References">References</a><br />
 +
      </tr>
 +
      <tr>
 +
        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Parts"><font size = 4>BIOBRICKS</font></a></td>
 +
      </tr>
 +
        <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Modeling"><font size = 4>MODELING</font></a></td>
 +
      </tr>
 +
      <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Notebook"><font size = 4>NOTEBOOK</font></a></td>
 +
      </tr>
 +
      <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/ETHICS"><font size = 4>ETHICS</font></a></td>
 +
      </tr>
 +
      <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Achievements"><font size = 4>JUDGING</font></a></td>
 +
      </tr>
 +
    <tr>
 +
      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Contact"><font size = 4>CONTACT</font></a></td>
 +
      </tr>
 +
    </table></td>
 +
<!---TEXT BOXES--->
 +
    <td><table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 +
  </td></tr>
-
== Project Details==
+
    <tr>
 +
    <td>
 +
  <tr>
 +
    <td>
 +
      <font size="5" face="Century Gothic, Arial, San Serif" color =#000033>
 +
              <b><i>BioBricks without Borders:
 +
</b></i></font>
 +
<br>
 +
<font size ="2.5" face="tahoma, sans-serif, verdana" color=#009900>
 +
  Investigating a multi-host BioBrick vector and secretion of cellular products</font>
 +
              <HR>
 +
 
 +
  <p class = "class">
 +
The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.
 +
  </P> </td></tr>
 +
 
 +
  </td>
 +
  </tr></tr>
 +
  </table>
 +
</td>
 +
  </tr>
 +
</table>
 +
</body>
 +
</html>
 +
<!---BACKGROUND FORMATTING--->
 +
<html>
 +
<head>
 +
<style>
 +
table {
 +
  background-color: #231f20;
 +
  font-color: white;
 +
  color:white;
 +
}
 +
a.menu {
 +
  background-color: #231f20;
 +
  color: white;
 +
  width: 12em;
 +
}
-
=== Protocols ===
+
.firstHeading {
 +
  color:white;
 +
}
 +
#bodyContent {
 +
background-color: #231f20;
 +
}
 +
#content {
 +
background-color: #231f20;
 +
}
 +
#footer-box {
 +
  background-color: #231f20;
 +
}
 +
p {
 +
  color:white;
 +
}
 +
body {
 +
  background-color:#231f20;
 +
}
 +
.firstHeading {
 +
  display:none;
 +
}
 +
</style>
 +
</head>
-
=== Part 2 ===
 
 +
</html>
 +
<!--- Background Formatting--->
 +
<html>
 +
<style type="text/css">
 +
body {background-color:#231f20; }
 +
#content{
 +
border:0px;
 +
}
-
=== The Experiments ===
+
</style>
-
 
+
-
 
+
</html>
-
 
+
<html>
-
=== Part 3 ===
+
<head>
-
 
+
<style type="text/css">
-
== Results ==
+
p.class{
 +
text-indent:1.0em;
 +
color: #231f20;
 +
font-weight : none;
 +
font-family:Tahoma, Arial, Sans-serif;
 +
font size="2.5";
 +
}
 +
p.margin
 +
{
 +
margin-top:0px;
 +
margin-bottom:0px;
 +
margin-right:50px;
 +
margin-left:50px;
 +
color: #231f20;
 +
font-weight : none;
 +
font-family:Tahoma, Arial, Sans-serif;
 +
font size="2.5";
 +
font-style: italic;
 +
}
 +
ul.circle {list-style-type:circle; color: #231f20;}
 +
</style>
 +
</head>

Latest revision as of 02:00, 22 October 2009

USU iGem

Untitled Document

PROJECT Abstract
Introduction
Broad-Host Vectors
Secretion
Experiments
Future Work
References
BioBricks without Borders:
Investigating a multi-host BioBrick vector and secretion of cellular products

The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.



Retrieved from "http://2009.igem.org/Team:Utah_State/Project"