Team:Chiba/Notebook/Calendar/26 September 2009

From 2009.igem.org

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=== PCR ===
=== PCR ===
*Template
*Template
-
1 : ECFP
+
1 : ECFP([http://partsregistry.org/Part:BBa_I6057 BBa_I6057])
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2 : mCherry
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2 : mCherry([http://partsregistry.org/Part:BBa_E2060 BBa_E2060])
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3 : V-YFP
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3 : V-YFP([http://partsregistry.org/Part:BBa_K084003 BBa_K084003])
-
4 : mRFP without Nco1 site
+
4 : mOrange([http://partsregistry.org/Part:BBa_E2050 BBa_E2050])
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5 : mOrange
+
5 : LacZ &alpha;([http://partsregistry.org/Part:BBa_T9003 BBa_T9003])
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6 : sfGFP
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6 : mRFP without Nco1 site
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PCR
PCR
-
 
-
 
=== Gel electrophoresis ===
=== Gel electrophoresis ===

Latest revision as of 05:56, 27 September 2009

>Go to the Notebook page

(25_September_2009 <|>27_September_2009)


Contents

E.coli Painting(3)

RFP, GFP. CFP

Examine limit of AHL generation(2)-2

Yesterday's operation is here.


  • Today's operation

11:35-

We transplanted E.coli to NC filter and put these on each plates.

And then we took pictures every 30 min.


Picture are here.


Pictures

11:35

Negative Control

1

2

3

8

4

5

6

7


12:05

Negative Control

1

2

3

8

4

5

6

7


12:35

Negative Control

1

2

3

8

4

5

6

7


13:05

Negative Control

1

2

3

8

4

5

6

7


13:35

Negative Control

1

2

3

8

4

5

6

7


14:05

Negative Control

1

2

3

8

4

5

6

7


14:35

Negative Control

1

2

3

8

4

5

6

7


15:05

Negative Control

1

2

3

8

4

5

6

7

Making colors

PCR

  • Template

1 : ECFP([http://partsregistry.org/Part:BBa_I6057 BBa_I6057])

2 : mCherry([http://partsregistry.org/Part:BBa_E2060 BBa_E2060])

3 : V-YFP([http://partsregistry.org/Part:BBa_K084003 BBa_K084003])

4 : mOrange([http://partsregistry.org/Part:BBa_E2050 BBa_E2050])

5 : LacZ α([http://partsregistry.org/Part:BBa_T9003 BBa_T9003])

6 : mRFP without Nco1 site


14:45

We drew up mixtures for PCR.


  • Element of mixtures
Template 1 μL
Primer(F) 2.5 μL
Primer(R) 2.5 μL
Buffer(x 10) 5 μL
dNTP 4 μL
DNA pol. 1 μL
dH2O 34 μL
Total 50 μL


15:20-

PCR

Gel electrophoresis

15:30

We made gel for electrophoresis.

agar 0.14 g

TAE 20 mL


17:25-

Gel electrophoresis(135 V, 27 min)

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