Team:USTC/Notebook/Lab Work/18 September 2009

From 2009.igem.org

(Difference between revisions)
(New page: {{:Team:USTC/Template/Header}} {{:Team:USTC/Notebook/Lab_Work}} == {{SUBPAGENAME}} == *k176060 in pSB1A2: correct. *k176066 in pSB1A3: 1-830bp correct. *k176067 in pSB1A3: correct. *k17607...)
 
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Line 5: Line 5:
*k176066 in pSB1A3: 1-830bp correct.
*k176066 in pSB1A3: 1-830bp correct.
*k176067 in pSB1A3: correct.
*k176067 in pSB1A3: correct.
-
*k176070 in pSB1A3: 实际是K176078，前822bp正确。
+
*k176070 in pSB1A3: result shows that it is k176078, 1-822bp correct.
-
*k176071 in pSB1A3: 前844bp正确。
+
*k176071 in pSB1A3: 1-844bp correct.
-
*k176074 in pSB1A3: 实际是K176075，前852bp正确。
+
*k176074 in pSB1A3: result shows that it is k176075, 1-852bp correct.
-
*k176075 in pSB1A3: 实际是K176074，前865bp正确。
+
*k176075 in pSB1A3: result shows that it is k176074, 1-865bp correct.
-
*k176078 in pSB1A3: 实际是K176070，前859bp正确。
+
*k176078 in pSB1A3: result shows that it is k176070, 1-859bp correct.
-
*k176079 in pSB1A3: 实际是K176075，前860bp正确。
+
*k176079 in pSB1A3: result shows that it is k176075, 1-860bp correct.
 +
*k176000 in pSB1A3: signal is too weak.
 +
*k176001 in pSB1Ak3: correct.
 +
*k176002 in pSB1A3: 1-839bp correct.
 +
*k176003 in pSB1A3: correct.
 +
*k176004 in pSB1A2: incorrect after 637bp, transposon IS10 right inserted here.
 +
*k176005 in pSB1A3: 1-840bp correct.
 +
*k176006 in pSB1Ak3: 1-838bp correct.
 +
*k176008 in K176007: correct.
 +
*k176009 in K176007: correct.
 +
*k176011 in pSB1A3: 1-858bp correct.
 +
*k176012 in pSB1A3: 1-277bp correct, overlap peaks after that. There is a good chance that it is transposon inserted.
 +
*k176013 in pSB1A3: 1-737bp correct.
 +
*k176014 in pSB1A3: 1-830bp correct.
 +
*k176015 in pSB1A3: 1-822bp correct.
 +
*k176016 in pSB1A3: 1-831bp correct.
 +
*k176018 in pSB1A3: 1-829bp correct.
 +
*k176019 in pSB1A3: 1-807bp correct.
 +
*k176020 in pSB1A3: 1-688bp correct. There is a good chance that 689bp is mutation.VR reaction again.
 +
 
 +
*k176020 in pSB1A3: correct.
 +
*k176081 in pSB1A3: 1-819bp correct.
 +
*k176082 in pSB1A3: 1-814bp correct.
 +
*k176083 in pSB1A3: 1-820bp correct.
 +
*k176084 in pSB1A3: result shows that it is k176086, 1-856bp correct.
 +
*k176085 in pSB1A3: 1-856bp correct.
 +
*k176086 in pSB1A3: result shows that it is k176084, 1-862bp correct.
 +
*k176087 in pSB1A3: 1-842bp correct.
 +
*k176035 in pSB1AK3: 1-615bp correct but 14-17bp,485bp unclear.
 +
*k176109 in pSB1A3: 23-848bp correct.
 +
*k176110 in pSB1A3: 1-560bp correct but a length of 90bp is unclear, overlap peaks.
 +
*k176108 in pSB1A3: 1-809bp correct but 63~65bp absence.

Latest revision as of 04:31, 1 October 2009

USTC
Home Team Project Modeling Parts Standard & Protocol Software Tool Human Practice Notebook

Team:USTC/Notebook/Lab Work/18 September 2009

Contents

We chose to use [http://ipadeln.com/ iPad] as our Electronic Lab Notebook. iPad is recommended on OWW [http://openwetware.org/wiki/OpenWetWare:Software/Projects/Lab_Notebooks/Technology].

Our work progress is also updated here.

May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/1_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 1] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/2_May_2009 2] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/3_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/4_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/5_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/6_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/7_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/8_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/9_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/10_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/11_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/12_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/13_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/14_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/15_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 15] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/16_May_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/17_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 17]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/18_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/19_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/20_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 20] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/21_May_2009 21] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/22_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/23_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/24_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 24]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/25_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/26_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/27_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/28_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/29_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/30_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/31_May_2009&preload=Team:USTC/Template/Lab_Work&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/1_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/2_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/3_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/4_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/5_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/6_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/7_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/8_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/9_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 9] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/10_June_2009 10] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/11_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/12_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/13_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 13] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/14_June_2009 14]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/15_June_2009 15] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/16_June_2009 16] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/17_June_2009 17] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/18_June_2009 18] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/19_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 19] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/20_June_2009 20] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/21_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/22_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/23_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/24_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/25_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/26_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/27_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/28_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/29_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/30_June_2009&preload=Team:USTC/Template/Lab_Work&action=edit 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/1_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/2_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 2] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/4_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/5_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/6_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 6] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/8_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/9_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/10_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/11_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/12_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 12]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/13_July_2009 13] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/14_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/15_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/16_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 16] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/17_July_2009 17] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/18_July_2009 18] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/19_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/20_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/21_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 21] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/22_July_2009 22] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/23_July_2009 23] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/24_July_2009 24] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/26_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 26]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/27_July_2009 27] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/28_July_2009 28] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/29_July_2009 29] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/30_July_2009&preload=Team:USTC/Template/Lab_Work&action=edit 30] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/1_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 1] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/2_August_2009 2]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/3_August_2009 3] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/4_August_2009 4] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/5_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/6_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/7_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/8_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 8] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/9_August_2009 9]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/10_August_2009 10] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/11_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 11] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/12_August_2009 12] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/13_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 13] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/15_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/16_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 16]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/17_August_2009 17] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/18_August_2009 18] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/19_August_2009 19] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/20_August_2009 20] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/21_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 21] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/22_August_2009 22] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/23_August_2009 23]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/24_August_2009 24] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/25_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/26_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/27_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/28_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 28] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/29_August_2009 29] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/30_August_2009 30]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/31_August_2009&preload=Team:USTC/Template/Lab_Work&action=edit 31]
September
MTWTFSS
  [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/1_September_2009 1] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/2_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/3_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/4_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 4] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/5_September_2009 5] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/6_September_2009 6]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/7_September_2009 7] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/8_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/9_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/10_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/11_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/12_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/13_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 13]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/14_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 14] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/15_September_2009 15] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/16_September_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/17_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 17] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/18_September_2009 18] [http://2009.igem.org/Team:USTC/Notebook/Lab_Work/19_September_2009 19] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/20_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 20]
[http://2009.igem.org/Team:USTC/Notebook/Lab_Work/21_September_2009 21] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/22_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/23_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/24_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/25_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/26_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/27_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/28_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/29_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/30_September_2009&preload=Team:USTC/Template/Lab_Work&action=edit 30]
October
MTWTFSS
      [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/1_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/2_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/3_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/4_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 4]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/5_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/6_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/7_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/8_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/9_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/10_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/11_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/12_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/13_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/14_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/15_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/16_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/17_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/18_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/19_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/20_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/21_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/22_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/23_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/24_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/25_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/26_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/27_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/28_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/29_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/30_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:USTC/Notebook/Lab_Work/31_October_2009&preload=Team:USTC/Template/Lab_Work&action=edit 31]

Team:USTC/Notebook/Lab Work/18 September 2009

  • k176060 in pSB1A2: correct.
  • k176066 in pSB1A3: 1-830bp correct.
  • k176067 in pSB1A3: correct.
  • k176070 in pSB1A3: result shows that it is k176078, 1-822bp correct.
  • k176071 in pSB1A3: 1-844bp correct.
  • k176074 in pSB1A3: result shows that it is k176075, 1-852bp correct.
  • k176075 in pSB1A3: result shows that it is k176074, 1-865bp correct.
  • k176078 in pSB1A3: result shows that it is k176070, 1-859bp correct.
  • k176079 in pSB1A3: result shows that it is k176075, 1-860bp correct.
  • k176000 in pSB1A3: signal is too weak.
  • k176001 in pSB1Ak3: correct.
  • k176002 in pSB1A3: 1-839bp correct.
  • k176003 in pSB1A3: correct.
  • k176004 in pSB1A2: incorrect after 637bp, transposon IS10 right inserted here.
  • k176005 in pSB1A3: 1-840bp correct.
  • k176006 in pSB1Ak3: 1-838bp correct.
  • k176008 in K176007: correct.
  • k176009 in K176007: correct.
  • k176011 in pSB1A3: 1-858bp correct.
  • k176012 in pSB1A3: 1-277bp correct, overlap peaks after that. There is a good chance that it is transposon inserted.
  • k176013 in pSB1A3: 1-737bp correct.
  • k176014 in pSB1A3: 1-830bp correct.
  • k176015 in pSB1A3: 1-822bp correct.
  • k176016 in pSB1A3: 1-831bp correct.
  • k176018 in pSB1A3: 1-829bp correct.
  • k176019 in pSB1A3: 1-807bp correct.
  • k176020 in pSB1A3: 1-688bp correct. There is a good chance that 689bp is mutation.VR reaction again.
  • k176020 in pSB1A3: correct.
  • k176081 in pSB1A3: 1-819bp correct.
  • k176082 in pSB1A3: 1-814bp correct.
  • k176083 in pSB1A3: 1-820bp correct.
  • k176084 in pSB1A3: result shows that it is k176086, 1-856bp correct.
  • k176085 in pSB1A3: 1-856bp correct.
  • k176086 in pSB1A3: result shows that it is k176084, 1-862bp correct.
  • k176087 in pSB1A3: 1-842bp correct.
  • k176035 in pSB1AK3: 1-615bp correct but 14-17bp,485bp unclear.
  • k176109 in pSB1A3: 23-848bp correct.
  • k176110 in pSB1A3: 1-560bp correct but a length of 90bp is unclear, overlap peaks.
  • k176108 in pSB1A3: 1-809bp correct but 63~65bp absence.
Retrieved from "http://2009.igem.org/Team:USTC/Notebook/Lab_Work/18_September_2009"