Team:USTC/Notebook/Lab Work/18 September 2009
From 2009.igem.org
(Difference between revisions)
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*k176011 in pSB1A3: 1-858bp correct. | *k176011 in pSB1A3: 1-858bp correct. | ||
*k176012 in pSB1A3: 1-277bp correct, overlap peaks after that. There is a good chance that it is transposon inserted. | *k176012 in pSB1A3: 1-277bp correct, overlap peaks after that. There is a good chance that it is transposon inserted. | ||
- | *k176013 in pSB1A3: 1-737bp correct. | + | *k176013 in pSB1A3: 1-737bp correct. |
*k176014 in pSB1A3: 1-830bp correct. | *k176014 in pSB1A3: 1-830bp correct. | ||
*k176015 in pSB1A3: 1-822bp correct. | *k176015 in pSB1A3: 1-822bp correct. | ||
Line 31: | Line 31: | ||
*k176020 in pSB1A3: correct. | *k176020 in pSB1A3: correct. | ||
+ | *k176081 in pSB1A3: 1-819bp correct. | ||
+ | *k176082 in pSB1A3: 1-814bp correct. | ||
+ | *k176083 in pSB1A3: 1-820bp correct. | ||
+ | *k176084 in pSB1A3: result shows that it is k176086, 1-856bp correct. | ||
+ | *k176085 in pSB1A3: 1-856bp correct. | ||
+ | *k176086 in pSB1A3: result shows that it is k176084, 1-862bp correct. | ||
+ | *k176087 in pSB1A3: 1-842bp correct. | ||
+ | *k176035 in pSB1AK3: 1-615bp correct but 14-17bp,485bp unclear. | ||
+ | *k176109 in pSB1A3: 23-848bp correct. | ||
+ | *k176110 in pSB1A3: 1-560bp correct but a length of 90bp is unclear, overlap peaks. | ||
+ | *k176108 in pSB1A3: 1-809bp correct but 63~65bp absence. |
Latest revision as of 04:31, 1 October 2009
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Team:USTC/Notebook/Lab Work/18 September 2009
Contents |
We chose to use [http://ipadeln.com/ iPad] as our Electronic Lab Notebook. iPad is recommended on OWW [http://openwetware.org/wiki/OpenWetWare:Software/Projects/Lab_Notebooks/Technology].
Our work progress is also updated here.
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Team:USTC/Notebook/Lab Work/18 September 2009
- k176060 in pSB1A2: correct.
- k176066 in pSB1A3: 1-830bp correct.
- k176067 in pSB1A3: correct.
- k176070 in pSB1A3: result shows that it is k176078, 1-822bp correct.
- k176071 in pSB1A3: 1-844bp correct.
- k176074 in pSB1A3: result shows that it is k176075, 1-852bp correct.
- k176075 in pSB1A3: result shows that it is k176074, 1-865bp correct.
- k176078 in pSB1A3: result shows that it is k176070, 1-859bp correct.
- k176079 in pSB1A3: result shows that it is k176075, 1-860bp correct.
- k176000 in pSB1A3: signal is too weak.
- k176001 in pSB1Ak3: correct.
- k176002 in pSB1A3: 1-839bp correct.
- k176003 in pSB1A3: correct.
- k176004 in pSB1A2: incorrect after 637bp, transposon IS10 right inserted here.
- k176005 in pSB1A3: 1-840bp correct.
- k176006 in pSB1Ak3: 1-838bp correct.
- k176008 in K176007: correct.
- k176009 in K176007: correct.
- k176011 in pSB1A3: 1-858bp correct.
- k176012 in pSB1A3: 1-277bp correct, overlap peaks after that. There is a good chance that it is transposon inserted.
- k176013 in pSB1A3: 1-737bp correct.
- k176014 in pSB1A3: 1-830bp correct.
- k176015 in pSB1A3: 1-822bp correct.
- k176016 in pSB1A3: 1-831bp correct.
- k176018 in pSB1A3: 1-829bp correct.
- k176019 in pSB1A3: 1-807bp correct.
- k176020 in pSB1A3: 1-688bp correct. There is a good chance that 689bp is mutation.VR reaction again.
- k176020 in pSB1A3: correct.
- k176081 in pSB1A3: 1-819bp correct.
- k176082 in pSB1A3: 1-814bp correct.
- k176083 in pSB1A3: 1-820bp correct.
- k176084 in pSB1A3: result shows that it is k176086, 1-856bp correct.
- k176085 in pSB1A3: 1-856bp correct.
- k176086 in pSB1A3: result shows that it is k176084, 1-862bp correct.
- k176087 in pSB1A3: 1-842bp correct.
- k176035 in pSB1AK3: 1-615bp correct but 14-17bp,485bp unclear.
- k176109 in pSB1A3: 23-848bp correct.
- k176110 in pSB1A3: 1-560bp correct but a length of 90bp is unclear, overlap peaks.
- k176108 in pSB1A3: 1-809bp correct but 63~65bp absence.