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Imperial College London/Notebook/1 October 2009
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===OD calibration=== | ===OD calibration=== | ||
*17th of September | *17th of September | ||
| - | * | + | *23rd of September |
| + | *25th of September | ||
| + | *Preliminary results show that cell number varies with OD, repetition of the experiment will allow to fit a curve to the graph: cell number vs OD. | ||
===Secondary carbon sources=== | ===Secondary carbon sources=== | ||
| + | *Running as we speak | ||
| + | |||
===GFP pH=== | ===GFP pH=== | ||
| + | *Data analysis | ||
| + | |||
===IPTG growth=== | ===IPTG growth=== | ||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 15:29, 1 October 2009
Contents |
Thursday the 1st of October
Testing team (update)
Harvard-GFP
- There is a difference in fluorescence between 28°C and 37°C after compensating for OD.
- Need to adjust the gain so that fluorescence doesn't saturate
- Runs: Wed 23rd September, 30th
OD calibration
- 17th of September
- 23rd of September
- 25th of September
- Preliminary results show that cell number varies with OD, repetition of the experiment will allow to fit a curve to the graph: cell number vs OD.
Secondary carbon sources
- Running as we speak
GFP pH
- Data analysis
IPTG growth
