Team:Freiburg bioware/Notebook/April

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FREiGEM

April

24th April 12:30 - 18:00 o'clock Manuel

Literaturrecherche zu FokI.
Entstandene Ideen:

three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

29th april 21o'clock Rüdiger

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@@ Line 79: / Line 85: @@
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-<h2 class="art-PostHeaderIcon-wrapper">April<span
+<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;<img
-  class="art-PostHeader"></span> </h2>
+  style="width: 28px; height: 25px;" alt=""
+ src="https://static.igem.org/mediawiki/2009/2/2a/Freiburg09_Post_tanne_2.png" />
+April
+<span class="art-PostHeader"></span> </h2>
 </div>
-<div style="text-align: center;" class="art-PostContent">
+<div class="art-PostContent">
-<br />
+<div style="text-align: center;"></div>
-<div style="text-align: left;">===24th april 12:30 - 18:00 o'clock Manuel===
+<h3>24th April 12:30 - 18:00 o'clock Manuel</h3>
+<div style="text-align: left;">Literaturrecherche zu FokI.<br />
-Literaturrecherche zu FokI.<br>
 Entstandene Ideen:
 <ul>
-<li>drei Oligo's: zwei für die Bindung der Monomere und eines zur Erzeugung der dsDNA an die FokI binden kann.</li>
+  <li>three oligo's: two for binding the monomers and one to
-<li>zwei Oligo's: eines zur Bindung des einen Monomers, das zweite zur Bindung des zweiten Monomers und zur Ausbildung der dsDNA (ein Monomer müsste hierfür inaktiviert werden).</li>
+create a dsDNA where FokI can bind.</li>
-<li>ein Oligo: beide Bindestellen der Monomere auf einem Oligo. Auch hier müsste ein Monomer inaktiviert sein.</li>
+  <li>two oligo's: one for binding one monomer, the second for
+binding
-Es wurde die 196 C-terminalen Aminosäuren von FokI verwendet. Primersequenzen sind vorhanden.<br>
+the second monomer and to create dsDNA (one monomer has to be
+inactivated).</li>
-<a href="Freiburg09_FokI_Zn-Finger_1996.pdf"</a><br><br><br>
+  <li>one oligo: both binding sites of the monomers on one oligo
+(also one monomer has to be inactivated).</li>
+</ul>
-===29th april 21o'clock Rüdiger===
+<br />
-<table>
+The 196 C-terminale aminoacids of FokI were used from other groups (see
-<tr><td><img src="Freiburg09_huebsche_fokI.jpg" name="Figure 1: FokI-Model, ribbon-style. DNA Strand is bound by two FOKI" width="400" height="400" alt="FokI bound to dsDNA" /></td>
+reference 1). Primersequences are also provided in the publication.<br />
-<td>[[Image:freiburg09_huebsche fokI_surface_render.jpg|none|thumb|Figure 2: FokI-Model surface rendering. DNA Strand is bound by two FOKI width="400" height="400"></td></tr>
+Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf
-<tr><td>[[Image:freiburg09_fokI_3oligos.JPG|none|thumb|Schema 3: 2Oligos, both with active fokI, one cutting DNA, the other cutting a third oligo|400x400px]]</td>
+<br />
-<td>[[Image:freiburg09_fokI_1inactive.JPG|none|thumb|Schema 4: 2Oligos, one with active fokI cutting DNA, the other inactive, just binding its own oligo|400x400px]]</td></tr>
+<br />
-<tr><td>[[Image:freiburg09_fokI_1oligo.JPG|none|thumb|Schema 5: 1Oligo with active FokI cutting DNA, the other inactive, just binding its own oligo|400x400px]]</td>
+<h3><a name="29"></a>29th april 21o'clock
-<td>[[Image:freiburg09_fokI_pna.JPG|none|thumb|Schema 6: 2Oligo, with active fokI cutting DNA, attached on both FokI is a specific PNA. Oligos must be designed to bind this sequence. It is to be discussed whether there is a third oligo required for 3d stabilisation of dna |400x400px]]</td></tr>
+R&uuml;diger</h3>
+<table style="text-align: left; width: 633px; height: 76px;"
+ border="0" cellpadding="0" cellspacing="2">
+  <tbody>
+    <tr>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/9/99/Freiburg09_huebsche_fokI.jpg" /></td>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/2/23/Freiburg09_huebsche_fokI_surface_render.jpg" /></td>
+    </tr>
+    <tr>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_fokI_3oligos.JPG" /></td>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/6/6e/Freiburg09_fokI_1inactive.JPG" /></td>
+    </tr>
+    <tr>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_fokI_1oligo.JPG" /></td>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/4/4a/Freiburg09_fokI_pna.JPG" /></td>
+    </tr>
+  </tbody>
 </table>
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