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| - | <html><p align=right><a href="https://2009.igem.org/Team:Imperial_College_London"><img style="vertical-align:bottom;" width="9%" src="http://www.clker.com/cliparts/b/4/5/9/12236111601800075995dynnamitt_home.svg.med.png"></a></html>
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| - | {{Imperial/09/Tabs/M2}}
| + | =[[Image:II09_Thumb_m2.png|40px]]<font size='5'><b>Module 2 - Acid Resistance Overview</b></font>= |
| - | =[[Image:II09_Thumb_m2.png|40px]]<font size='5'><b>Module 2 - Encapsulsation Overview</b></font>= | + | |
| | [[Image:II09_TimelineM2.png |center| 600px]] | | [[Image:II09_TimelineM2.png |center| 600px]] |
| - | =Encapsulation Rationale=
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| - | In nature, encapsulation pathways such as spore formation, aliginate biosynthesis and colanic acid production all share one common feature: they require a large number of genes. For this reason, we decided that the best way encapsulate our chassis was via the modulation of an existing pathway.
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| - | <i>E.coli</i> naturally produces a harmless acid resistant polymer known as colanic acid. By tapping into the pathway that initiates colanic acid biosynthesis, we can turn on its production via the modulation of a gene called RcsB.
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| - | In nature, colanic acid acts as a binding agent between individual cells over which a biofilm can be formed. While colanic acid itself is harmless, biofilm formation is associated with the production of a number of virulence factors. To prevent biofilm formation from occuring, we have tapped into a second pathway such that our cells become locked into colanic acid production. The gene responsible for preventing biofilm formation is a transcription factor called YgiV.
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| - | In nature, colanic acid is associated with but not attached to the cell surface. To facilitate whole cell encapsulation, we have modified a third pathway to fix the colanic acid to the surface of the cell. This involves an enzyme called Rfal.
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| | + | {{Imperial/09/Division}} |
| | + | <center> |
| | + | ===Module 2 - Encapsulation=== |
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| - | <html><a href="https://2009.igem.org/Team:Imperial_College_London/M2/ genes"><img width= 90px src="http://i691.photobucket.com/albums/vv271/dk806/ II09_Learnmore.png" align=" left"></a></ html>< br>< br> About RcsB, YgiV and Rfal. | + | <html ><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2 "><img style="vertical-align:bottom;" width="20%" src="http:/ /i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/M2/FreezeDrying"><img style="vertical-align:bottom;" width= "20%" src="http://i691.photobucket.com/albums/vv271/dk806/ II09_freezedrying.png" ></a><a |
| | + | href="https://2009.igem.org/Team:Imperial_College_London/M2/Genetic"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_geneticcircuit1.png"></a><a |
| | + | href="https://2009.igem.org/Team:Imperial_College_London/M2/Wetlab"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Wetlabmainimage9.png"></a><html><a |
| | + | href="https://2009.igem.org/Team:Imperial_College_London/M2/Modelling"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage6.png"></a><center></html> |
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| | + | <html><table border="0" style="background-color:transparent;" width="100%"> |
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| - | < html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M1"><img width =150px src=" https://static.igem.org/mediawiki/2009/4/4d/II09_M2ArrowLeft.png">< /a><a href="https://2009.igem.org/Team:Imperial_College_London/ M3">< img width=150px src="https:/ /static.igem.org/mediawiki/2009/a/af/II09_M2ArrowRight.png"></a></center> | + | < td width=" 20%">< center><a href="https://2009.igem.org/Team:Imperial_College_London/ M2">< b>Module 2 Overview</ b></a></center></ td> |
| - | </html> | + | |
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| | + | <td width="20%"><center><a href="/Team:Imperial_College_London/M2/FreezeDrying"><b>Freeze Drying</b></a></center></td> |
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| - | {{Imperial/09/Division}}
| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2/Genetic"><b>Genetic Circuit</b></a></center></td> |
| - | <center><b>Module 2 - Encapsulation</b></center>
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| - | < html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2/Genetic"><img style="vertical-align:bottom;" width=" 23% " src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png">< /a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/M2/Wetlab">< img style="vertical-align:bottom;" width="23%" src="http:/ /i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage4.png"></a>< a href="https:// 2009.igem.org/Team:Imperial_College_London/M2/Modelling">< img style="vertical-align:bottom;" width=" 23% " src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage5.png">< /a><html><a | + | < td width=" 20%">< center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/M2/Wetlab">< b>Wet Lab</ b></a></ center></ td> |
| - | href="https://2009.igem.org/Team:Imperial_College_London/M2/Results"><img style="vertical-align:bottom;" width="27%" src="https://static.igem.org/mediawiki/2009/2/20/II09_WorldMap.png"></a><center></html> | + | |
| | + | < td width=" 20%">< center><a |
| | + | href="https://2009.igem.org/Team:Imperial_College_London/M2/Modelling"><b>Modelling</b></a></center></td> |
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| - | <html><table border="0" style="background-color:transparent;" width="100%">
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| - | <tr><td width="0%"></td>
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| - | <td width="25%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2/Genetic"><b>Genetic Circuit</b></a></center></td>
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| - | <td width="25%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/M2/Wetlab"><b>WetLab</b></a></center></td>
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| - | <td width="25%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2/Modelling"><b>Modelling</b></a></center></td>
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| - | <td width="25%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2/Results"><b>Results</b></a></center></td>
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| | </tr></table></html> | | </tr></table></html> |
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| | + | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2#Module_2_Contents"><img width=150px src="https://static.igem.org/mediawiki/2009/1/10/II09_TourArrow.png"></a> |
| | + | </html> |
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Module 2 - Acid Resistance Overview
