Team:Freiburg bioware/Notebook/April

From 2009.igem.org

(Difference between revisions)
 
(9 intermediate revisions not shown)
Line 5: Line 5:
  content="text/html; charset=UTF-8" />
  content="text/html; charset=UTF-8" />
   <meta http-equiv="X-UA-Compatible" content="IE=EmulateIE7" />
   <meta http-equiv="X-UA-Compatible" content="IE=EmulateIE7" />
-
   <title>FREiGEM 2009</title>
+
   <title>FREiGEM</title>
   <script type="text/javascript" src="script.js"></script>
   <script type="text/javascript" src="script.js"></script>
-
   <link rel="stylesheet" href="/wiki/index.php?title=User:Maximi/general_style.css&action=raw&ctype=text/css"
+
   <link rel="stylesheet" href="/wiki/index.php?title=User:Maximi/new_general_style.css&action=raw&ctype=text/css"
  type="text/css" media="screen" />
  type="text/css" media="screen" />
 +
<!--[if IE 6]><link rel="stylesheet" href="style.ie6.css" type="text/css" media="screen" /><![endif]--><!--[if IE 7]><link rel="stylesheet" href="style.ie7.css" type="text/css" media="screen" /><![endif]-->
</head>
</head>
<body>
<body>
Line 25: Line 26:
<div class="art-Sheet-body">
<div class="art-Sheet-body">
<div class="art-Header">
<div class="art-Header">
-
<div class="art-Header-png"></div>
 
<div class="art-Header-jpeg"></div>
<div class="art-Header-jpeg"></div>
</div>
</div>
Line 32: Line 32:
<div class="r"></div>
<div class="r"></div>
<ul class="art-menu">
<ul class="art-menu">
-
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware" class="active"><span
+
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware"><span class="l"></span><span
 +
class="r"></span><span class="t">Home</span></a></li>
 +
  <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team"><span
  class="l"></span><span class="r"></span><span
  class="l"></span><span class="r"></span><span
-
  class="t">Home</span></a></li>
+
  class="t">The Team</span></a>
-
  <li><a href="#"><span class="l"></span><span
+
-
class="r"></span><span class="t">The Team</span></a>
+
     <ul>
     <ul>
-
       <li><a
+
       <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Overview</a></li>
-
href="https://2009.igem.org/Team:Freiburg_bioware/Team">Overview</a></li>
+
       <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team/Portraits">Portraits</a></li>
-
       <li><a href="#">Fotos</a></li>
+
     </ul>
     </ul>
   </li>
   </li>
Line 46: Line 45:
  class="r"></span><span class="t">The
  class="r"></span><span class="t">The
Project</span></a></li>
Project</span></a></li>
-
   <li><a href="#"><span class="l"></span><span
+
   <li><a
-
class="r"></span><span class="t">Parts</span></a>
+
href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice"><span
 +
class="l"></span><span class="r"></span><span
 +
class="t">Human
 +
Practice</span></a>
     <ul>
     <ul>
-
       <li><a href="#">1</a>
+
       <li><a href="#">D'oh! Something's missing here.</a>
         <ul>
         <ul>
 +
          <li><a href="#">1</a> </li>
           <li><a href="#">2</a> </li>
           <li><a href="#">2</a> </li>
           <li><a href="#">3</a> </li>
           <li><a href="#">3</a> </li>
-
          <li><a href="#">4</a> </li>
 
         </ul>
         </ul>
       </li>
       </li>
 +
      <li><a href="#">4</a></li>
       <li><a href="#">5</a></li>
       <li><a href="#">5</a></li>
-
      <li><a href="#">6</a></li>
 
     </ul>
     </ul>
   </li>
   </li>
-
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span
+
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook" class="active"><span class="l"></span><span
  class="r"></span><span class="t">Notebook</span></a></li>
  class="r"></span><span class="t">Notebook</span></a></li>
 +
  <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span
 +
class="l"></span><span class="r"></span><span
 +
class="t">Collaboration</span></a></li>
</ul>
</ul>
</div>
</div>
Line 79: Line 85:
<div class="art-Post-inner">
<div class="art-Post-inner">
<div class="art-PostMetadataHeader">
<div class="art-PostMetadataHeader">
-
<h2 class="art-PostHeaderIcon-wrapper">April<span
+
<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;<img
-
  class="art-PostHeader"></span> </h2>
+
  style="width: 28px; height: 25px;" alt=""
 +
src="https://static.igem.org/mediawiki/2009/2/2a/Freiburg09_Post_tanne_2.png" />
 +
April
 +
<span class="art-PostHeader"></span> </h2>
</div>
</div>
-
<div style="text-align: center;" class="art-PostContent">
+
<div class="art-PostContent">
-
<br />
+
<div style="text-align: center;"></div>
-
<div style="text-align: left;">
+
<h3>24th April 12:30 - 18:00 o'clock Manuel</h3>
-
 
+
<div style="text-align: left;">Literaturrecherche zu FokI.<br />
-
<h3>24th april 12:30 - 18:00 o'clock Manuel</h3>
+
-
 
+
-
Literaturrecherche zu FokI.<br>
+
Entstandene Ideen:
Entstandene Ideen:
-
 
<ul>
<ul>
-
<li>three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.</li>
+
  <li>three oligo's: two for binding the monomers and one to
-
<li>two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).</li>
+
create a dsDNA where FokI can bind.</li>
-
<li>one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).</li>
+
  <li>two oligo's: one for binding one monomer, the second for
 +
binding
 +
the second monomer and to create dsDNA (one monomer has to be
 +
inactivated).</li>
 +
  <li>one oligo: both binding sites of the monomers on one oligo
 +
(also one monomer has to be inactivated).</li>
</ul>
</ul>
-
<br>
+
<br />
-
The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.<br>
+
The 196 C-terminale aminoacids of FokI were used from other groups (see
-
 
+
reference 1). Primersequences are also provided in the publication.<br />
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf
-
<br><br>
+
<br />
-
 
+
<br />
-
 
+
<h3><a name="29"></a>29th april 21o'clock
-
<h3>29th april 21o'clock Rüdiger</h3>
+
R&uuml;diger</h3>
-
 
+
<table style="text-align: left; width: 633px; height: 76px;"
-
<table>
+
border="0" cellpadding="0" cellspacing="2">
-
<tr>
+
  <tbody>
-
<td><img src="https://static.igem.org/mediawiki/2009/9/99/Freiburg09_huebsche_fokI.jpg" name="Figure 1: FokI-Model, ribbon-style. DNA Strand is bound by two FOKI" width="300" height="300" alt="FokI bound to dsDNA" /></td>
+
    <tr>
-
 
+
      <td><img style="width: 300px; height: 300px;"
-
<td><img src="https://static.igem.org/mediawiki/2009/2/23/Freiburg09_huebsche_fokI_surface_render.jpg" name="Figure 2: FokI-Model surface rendering. DNA Strand is bound by two FOKI" width="300" height="300" /></td>
+
alt=""
-
</tr>
+
src="https://static.igem.org/mediawiki/2009/9/99/Freiburg09_huebsche_fokI.jpg" /></td>
-
 
+
      <td><img style="width: 300px; height: 300px;"
-
<tr>
+
alt=""
-
<td><img src="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_fokI_3oligos.JPG" name="Schema 3: 2Oligos, both with active fokI, one cutting DNA, the other cutting a third oligo" width="300" height="300" /></td>
+
src="https://static.igem.org/mediawiki/2009/2/23/Freiburg09_huebsche_fokI_surface_render.jpg" /></td>
-
 
+
    </tr>
-
<td><img src="https://static.igem.org/mediawiki/2009/6/6e/Freiburg09_fokI_1inactive.JPG" name="Schema 4: 2Oligos, one with active fokI cutting DNA, the other inactive, just binding its own oligo" width="300" height="300" /></td>
+
    <tr>
-
</tr>
+
      <td><img style="width: 300px; height: 300px;"
-
 
+
alt=""
-
<tr>
+
src="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_fokI_3oligos.JPG" /></td>
-
<td><img src="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_fokI_1oligo.JPG" name="Schema 5: 1Oligo with active FokI cutting DNA, the other inactive, just binding its own oligo" width="300" height="300" /></td>
+
      <td><img style="width: 300px; height: 300px;"
-
 
+
alt=""
-
<td><img src="https://static.igem.org/mediawiki/2009/4/4a/Freiburg09_fokI_pna.JPG" name="Schema 6: 2Oligo, with active fokI cutting DNA, attached on both FokI is a specific PNA. Oligos must be designed to bind this sequence. It is to be discussed whether there is a third oligo required for 3d stabilisation of dna" width="300" height="300" /></td>
+
src="https://static.igem.org/mediawiki/2009/6/6e/Freiburg09_fokI_1inactive.JPG" /></td>
-
</tr>
+
    </tr>
 +
    <tr>
 +
      <td><img style="width: 300px; height: 300px;"
 +
alt=""
 +
src="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_fokI_1oligo.JPG" /></td>
 +
      <td><img style="width: 300px; height: 300px;"
 +
alt=""
 +
src="https://static.igem.org/mediawiki/2009/4/4a/Freiburg09_fokI_pna.JPG" /></td>
 +
    </tr>
 +
  </tbody>
</table>
</table>
-
 
-
<br>
 
</div>
</div>
-
<div style="text-align: left;"><br />
 
-
</div>
 
-
<div style="text-align: center;"><br />
 
<br />
<br />
-
</div>
 
</div>
</div>
<div class="cleared"></div>
<div class="cleared"></div>
Line 137: Line 150:
</div>
</div>
</div>
</div>
-
<div class="art-Post">
 
-
<div class="art-PostContent"><br />
 
<br />
<br />
</div>
</div>
-
<div class="art-Post-inner">
+
</div>
<div class="cleared"></div>
<div class="cleared"></div>
 +
<div class="art-Footer">
 +
<div class="art-Footer-inner">
 +
<div class="art-Footer-text">
 +
<p>contact:&nbsp; <a
 +
href="mailto:freigem09@googlemail.com">freigem09@googlemail.com</a><br />
 +
</p>
</div>
</div>
</div>
</div>
 +
<div class="art-Footer-background"></div>
</div>
</div>
</div>
</div>
-
<br />
 
</div>
</div>
-
</div>
 
-
<div class="cleared"></div>
 
</div>
</div>
</body>
</body>
</html>
</html>

Latest revision as of 10:23, 20 October 2009

FREiGEM

  April

24th April 12:30 - 18:00 o'clock Manuel

Literaturrecherche zu FokI.
Entstandene Ideen:
  • three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
  • two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
  • one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

29th april 21o'clock Rüdiger


contact:  freigem09@googlemail.com

Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/Notebook/April"