From 2009.igem.org
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| ===='''May 20, 2009'''==== | | ===='''May 20, 2009'''==== |
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- | '''Ex 2:''' growth on following plates | + | '''Ex 2:''' Growth on following plates |
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| 1. pBAD33 –Cm resistance | | 1. pBAD33 –Cm resistance |
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Line 58: |
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- | '''Other:''' Made antibiotics: Amp, Cm, Kan | + | '''Other:''' |
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- | Research
| + | Made antibiotics: Amp, Cm, Kan |
| + | |
| + | Research |
| | | |
| | | |
| '''Ex 1:''' MiniPrep of NudF and YhfR acroding to miniprep protocol | | '''Ex 1:''' MiniPrep of NudF and YhfR acroding to miniprep protocol |
- | Results: out of RNAase and got bad results from our gel | + | |
| + | Results: out of RNAase and got bad results from our gel |
| + | |
| + | [[Image:gel 05-20.jpg]] |
| + | |
| + | |
| + | - mini preparation of pNudF and pYhfR according to the mini prep protocol of Scott Walhovd, bad results b/c no RNAase - NP |
| + | |
| + | - inoculated liquid cultures of Dan's plasmids from yesterday in LB media with appropriate antibiotics - NP |
| + | |
| + | - made freezer stocks of pNudF and pYhfR - NP |
Latest revision as of 06:28, 20 October 2009
Calendar
April
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M | T | W | T | F | S | S
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_April_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_April_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_April_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_April_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_April_2009&action=edit 5]
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[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_April_2009&action=edit 6]
| [http://2009.igem.org/Wisconsin-Madison/7_April_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_April_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_April_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_April_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_April_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_April_2009&action=edit 12]
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[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/13_April_2009&action=edit 13]
| [http://2009.igem.org/Wisconsin-Madison/14_April_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_April_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_April_2009&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/17_April_2009&action=edit 17]
| [http://2009.igem.org/Wisconsin-Madison/18_April_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_April_2009 19]
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[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/20_April_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_April_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_April_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_April_2009&action=edit 23]
| [http://2009.igem.org/Wisconsin-Madison/24_April_2009 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_April_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_April_2009&action=edit 26]
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[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_April_2009&action=edit 27]
| [http://2009.igem.org/Wisconsin-Madison/28_April_2009 28]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_April_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_April_2009&action=edit 30]
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May
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M | T | W | T | F | S | S
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_May_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_May_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_May_2009&action=edit 3]
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[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_May_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_May_2009&action=edit 5]
| [http://2009.igem.org/Wisconsin-Madison/6_May_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_May_2009&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_May_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_May_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_May_2009&action=edit 10]
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[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_May_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_May_2009&action=edit 12]
| [http://2009.igem.org/Wisconsin-Madison/13_May_2009 13]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_May_2009&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_May_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_May_2009&action=edit 16]
| [http://2009.igem.org/Wisconsin-Madison/17_May_2009 17]
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[http://2009.igem.org/Wisconsin-Madison/18_May_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_May_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_May_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_May_2009 21]
| [http://2009.igem.org/Wisconsin-Madison/22_May_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_May_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_May_2009&action=edit 24]
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[http://2009.igem.org/Wisconsin-Madison/25_May_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_May_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_May_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_May_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_May_2009 29]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_May_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_May_2009&action=edit 31]
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June
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M | T | W | T | F | S | S
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[http://2009.igem.org/Wisconsin-Madison/1_June_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_June_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_June_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_June_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_June_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_June_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_June_2009&action=edit 7]
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[http://2009.igem.org/Wisconsin-Madison/8_June_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_June_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_June_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_June_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_June_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_June_2009 13]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_June_2009&action=edit 14]
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[http://2009.igem.org/Wisconsin-Madison/15_June_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_June_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_June_2009 17]
| [http://2009.igem.org/Wisconsin-Madison/18_June_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_June_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_June_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_June_2009 21]
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[http://2009.igem.org/Wisconsin-Madison/22_June_2009 22]
| [http://2009.igem.org/Wisconsin-Madison/23_June_2009 23]
| [http://2009.igem.org/Wisconsin-Madison/24_June_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_June_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_June_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_June_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_June_2009 28]
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[http://2009.igem.org/Wisconsin-Madison/29_June_2009 29]
| [http://2009.igem.org/Wisconsin-Madison/30_June_2009 30]
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July
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Wisconsin-Madison/1_July_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_July_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_July_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_July_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_July_2009 5]
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[http://2009.igem.org/Wisconsin-Madison/6_July_2009 6]
| [http://2009.igem.org/Wisconsin-Madison/7_July_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_July_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_July_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_July_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_July_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_July_2009 12]
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[http://2009.igem.org/Wisconsin-Madison/13_July_2009 13]
| [http://2009.igem.org/Wisconsin-Madison/14_July_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_July_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_July_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/19_July_2009&action=edit 19]
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[http://2009.igem.org/Wisconsin-Madison/20_July_2009 20]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_July_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_July_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_July_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_July_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_July_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_July_2009&action=edit 26]
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[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_July_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_July_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_July_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_July_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_July_2009&action=edit 31]
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August
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Wisconsin-Madison/1_August_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_August_2009 2]
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[http://2009.igem.org/Wisconsin-Madison/3_August_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_August_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_August_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_August_2009 6]
| [http://2009.igem.org/Wisconsin-Madison/7_August_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_August_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_August_2009 9]
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[http://2009.igem.org/Wisconsin-Madison/10_August_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_August_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_August_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_August_2009 13]
| [http://2009.igem.org/Wisconsin-Madison/14_August_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_August_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_August_2009 16]
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[http://2009.igem.org/Wisconsin-Madison/17_August_2009 17]
| [http://2009.igem.org/Wisconsin-Madison/18_August_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_August_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_August_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_August_2009 21]
| [http://2009.igem.org/Wisconsin-Madison/22_August_2009 22]
| [http://2009.igem.org/Wisconsin-Madison/23_August_2009 23]
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[http://2009.igem.org/Wisconsin-Madison/24_August_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_August_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_August_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_August_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_August_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_August_2009 29]
| [http://2009.igem.org/Wisconsin-Madison/30_August_2009 30]
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[http://2009.igem.org/Wisconsin-Madison/31_August_2009 31]
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September
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Wisconsin-Madison/1_September_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_September_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_September_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_September_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_September_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_September_2009 6]
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[http://2009.igem.org/Wisconsin-Madison/7_September_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_September_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_September_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_September_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_September_2009&action=edit 11]
| [http://2009.igem.org/Wisconsin-Madison/12_September_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_September_2009 13]
|
[http://2009.igem.org/Wisconsin-Madison/14_September_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_September_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_September_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_September_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_September_2009&action=edit 18]
| [http://2009.igem.org/Wisconsin-Madison/19_September_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_September_2009 20]
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[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_September_2009&action=edit 21]
| [http://2009.igem.org/Wisconsin-Madison/22_September_2009 22]
| [http://2009.igem.org/Wisconsin-Madison/23_September_2009 23]
| [http://2009.igem.org/Wisconsin-Madison/24_September_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_September_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_September_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_September_2009&action=edit 27]
|
[http://2009.igem.org/Wisconsin-Madison/28_September_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_September_2009 29]
| [http://2009.igem.org/Wisconsin-Madison/30_September_2009 30]
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October
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Wisconsin-Madison/1_October_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_October_2009&action=edit 2]
| [http://2009.igem.org/Wisconsin-Madison/3_October_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_October_2009 4]
|
[http://2009.igem.org/Wisconsin-Madison/5_October_2009 5]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_October_2009&action=edit 6]
| [http://2009.igem.org/Wisconsin-Madison/7_October_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_October_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_October_2009&action=edit 9]
| [http://2009.igem.org/Wisconsin-Madison/10_October_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_October_2009&action=edit 11]
|
[http://2009.igem.org/Wisconsin-Madison/12_October_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_October_2009 13]
| [http://2009.igem.org/Wisconsin-Madison/14_October_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_October_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_October_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_October_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_October_2009&action=edit 18]
|
[http://2009.igem.org/Wisconsin-Madison/19_October_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_October_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_October_2009 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_October_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_October_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_October_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_October_2009&action=edit 25]
|
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_October_2009&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_October_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_October_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_October_2009&action=edit 29]
| [http://2009.igem.org/Wisconsin-Madison/30_October_2009 30]
| [http://2009.igem.org/Wisconsin-Madison/31_October_2009 31]
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May 20, 2009
Ex 2: Growth on following plates
1. pBAD33 –Cm resistance
2. pTrc 99A – Amp resistance
3. pBt2BAD18 – Kan resistance
Made liquid cultures of the three plasmids
Other:
Made antibiotics: Amp, Cm, Kan
Research
Ex 1: MiniPrep of NudF and YhfR acroding to miniprep protocol
Results: out of RNAase and got bad results from our gel
- mini preparation of pNudF and pYhfR according to the mini prep protocol of Scott Walhovd, bad results b/c no RNAase - NP
- inoculated liquid cultures of Dan's plasmids from yesterday in LB media with appropriate antibiotics - NP
- made freezer stocks of pNudF and pYhfR - NP
|