"

 

From 2009.igem.org

Revision as of 14:32, 14 October 2009 (view source) Calvin (Talk | contribs) ← Older edit		Latest revision as of 21:55, 20 October 2009 (view source) Calvin (Talk | contribs)
Line 264:		Line 264:
	<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li>		<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li>
	<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li>		<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li>
-	<li><a class="mainlink" href="/Team:ULB-Brussels/Modeling">Modeling</a></li>	+	<li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li>
	<li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li>		<li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li>
	<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li>		<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li>

---

Latest revision as of 21:55, 20 October 2009

July M T W T F S S     [http://2009.igem.org/ULB/1_July_2009 1] [http://2009.igem.org/ULB/2_July_2009 2] [http://2009.igem.org/wiki/index.php?title=ULB/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=ULB/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=ULB/5_July_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=ULB/6_July_2009&action=edit 6] [http://2009.igem.org/ULB/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_July_2009&action=edit 8] [http://2009.igem.org/ULB/9_July_2009 9] [http://2009.igem.org/wiki/index.php?title=ULB/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=ULB/11_July_2009&action=edit 11] [http://2009.igem.org/ULB/12_July_2009 12] [http://2009.igem.org/wiki/index.php?title=ULB/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=ULB/14_July_2009&action=edit 14] [http://2009.igem.org/ULB/15_July_2009 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_July_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_July_2009&action=edit 19] [http://2009.igem.org/ULB/20_July_2009 20] [http://2009.igem.org/ULB/21_July_2009 21] [http://2009.igem.org/ULB/22_July_2009 22] [http://2009.igem.org/ULB/23_July_2009 23] [http://2009.igem.org/ULB/24_July_2009 24] [http://2009.igem.org/ULB/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_July_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=ULB/28_July_2009&action=edit 28] [http://2009.igem.org/ULB/29_July_2009 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=ULB/31_July_2009&action=edit 31]
August M T W T F S S           [http://2009.igem.org/wiki/index.php?title=ULB/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_August_2009&action=edit 2] [http://2009.igem.org/ULB/3_August_2009 3] [http://2009.igem.org/ULB/4_August_2009 4] [http://2009.igem.org/wiki/index.php?title=ULB/5_August_2009&action=edit 5] [http://2009.igem.org/ULB/6_August_2009 6] [http://2009.igem.org/ULB/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=ULB/9_August_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=ULB/10_August_2009&action=edit 10] [http://2009.igem.org/ULB/11_August_2009 11] [http://2009.igem.org/ULB/12_August_2009 12] [http://2009.igem.org/ULB/13_August_2009 13] [http://2009.igem.org/ULB/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_August_2009&action=edit 16] [http://2009.igem.org/ULB/17_August_2009 17] [http://2009.igem.org/ULB/18_August_2009 18] [http://2009.igem.org/ULB/19_August_2009 19] [http://2009.igem.org/ULB/20_August_2009 20] [http://2009.igem.org/ULB/21_August_2009 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_August_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=ULB/24_August_2009&action=edit 24] [http://2009.igem.org/ULB/25_August_2009 25] [http://2009.igem.org/ULB/26_August_2009 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_August_2009&action=edit 27] [http://2009.igem.org/ULB/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_August_2009&action=edit 30] [http://2009.igem.org/ULB/31_August_2009 31]
September M T W T F S S   [http://2009.igem.org/ULB/1_September_2009 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_September_2009&action=edit 2] [http://2009.igem.org/ULB/3_September_2009 3] [http://2009.igem.org/ULB/4_September_2009 4] [http://2009.igem.org/ULB/5_September_2009 5] [http://2009.igem.org/wiki/index.php?title=ULB/6_September_2009&action=edit 6] [http://2009.igem.org/ULB/7_September_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_September_2009&action=edit 8] [http://2009.igem.org/ULB/9_September_2009 9] [http://2009.igem.org/ULB/10_September_2009 10] [http://2009.igem.org/ULB/11_September_2009 11] [http://2009.igem.org/ULB/12_September_2009 12] [http://2009.igem.org/wiki/index.php?title=ULB/13_September_2009&action=edit 13] [http://2009.igem.org/ULB/14_September_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_September_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=ULB/20_September_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=ULB/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=ULB/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=ULB/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_September_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=ULB/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_September_2009&action=edit 30]

Contents 1 July 12, 2009 1.1 Primers design for hfsG et hfsH genes 1.1.1 BBa_R0011 (promoter) : 1.1.2 RBS BBa_B0034 : 1.1.3 hfsG gene : 1.1.4 hfsH gene : 1.1.5 RBS + RFP : 1.1.6 BBa_B0015 terminator : 1.2 Sequences 1.2.1 Promoter (55 bp) 1.2.2 RBS (12 pb) 1.2.3 RBS + RFP (681 bp) 1.2.4 Terminator (129 bp) 1.2.5 Plasmid (3165 bp)

July 12, 2009

Forward and reverse primers are designed and ordered.

Primers design for hfsG et hfsH genes

BBa_R0011 (promoter) :

PREFIX :

=> GAATTCATGAGATCT aattgtgagcggataaca

SUFFIX :

<= ctcgagttaggatcc TGTGCTCAGTATCTTGTT

RBS BBa_B0034 :

PREFIX :

=> GAATTCATGAGATCT AAAGAG

SUFFIX :

<= ctcgagttaggatcc TTTCTC

hfsG gene :

PREFIX :

=> GAATTCATGAGATCT atgaacgcgcccgtcaacgagctgcgactggagaacgccg

SUFFIX :

<= cagacggcctcgctgtaga

hfsH gene :

PREFIX :

=> atgccgatggagttcgagaag

SUFFIX :

<= ctcgagttaggatcc ttagagcccgatcc

RBS + RFP :

PREFIX :

=> GAATTCATGAGATCT aaagaggagaaatactag

SUFFIX :

<= ctcgagttaggatcc GCGATCTACACTAGCACT

BBa_B0015 terminator :

PREFIX :

=> GAATTCATGAGATCT ccaggcatcaaataaaac

SUFFIX :

<= ctcgagttaggatcc TATAAACGCAGAAAGGCC

Sequences

Promoter (55 bp)

aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca

RBS (12 pb)

aaagaggagaaa

RBS + RFP (681 bp)

aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgt
atggaaggttccgttaacggtcacgagttcgAaatcgaaggtgaaggtgaaggtcgtccgtacgaaggt
acccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatc
acctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt
gcttaataacgctgatagtgctagtgtagatcgc

Terminator (129 bp)

ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

Plasmid (3165 bp)

ggatcctaactcgaggttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgt
aaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgat
atctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagcc
ataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgag
ccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggt
gagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctga
tgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatc
catttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaa
tttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaa
ctcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgcctt
gtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaa
agacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttc
actaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcc
tttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacacc
ataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttc
tttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatg
ctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatct
caattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtcct
tttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctag
accctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatag
aataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtc
agttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccc
taaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatca
ggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggg
gtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccg
tcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagc
agttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggct
aatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattc
tcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcatt
actggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcag
tgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc
ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag
ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcctt
cggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgca
taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca
tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacc
gctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac
cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaa
atgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgag
cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgcc
acctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaa
tttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcatgagatct

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers