Team:UNIPV-Pavia/Methods Materials/Glycerol stock

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(New page: '''Gycerol stock''' *Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol *Leave at -20°C for one day *Move to -80°C the day after)
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<h1>Protocols</h1>
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*[[Team:UNIPV-Pavia/Methods_Materials/Lb|Lb medium]]
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*[[Team:UNIPV-Pavia/Methods_Materials/Transformation|Transformation]]
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*[[Team:UNIPV-Pavia/Methods_Materials/XGal|X-Gal staining protocol for beta galactosidase (blue/white screening)]]
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*[[Team:UNIPV-Pavia/Methods_Materials/Glycerol stock|Glycerol stock]]
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*[[Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction|Plasmid extraction]]
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*[[Team:UNIPV-Pavia/Methods_Materials/Digestion|BioBrick digestion with restriction enzymes]]
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*[[Team:UNIPV-Pavia/Methods_Materials/GelExtraction|DNA gel extraction]]
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*[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]]
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*[[Team:UNIPV-Pavia/Methods_Materials/Pcr|PCR]]
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'''Gycerol stock'''  
'''Gycerol stock'''  
*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
*Leave at -20°C for one day
*Leave at -20°C for one day
*Move to -80°C the day after
*Move to -80°C the day after
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Revision as of 14:49, 22 June 2009

EthanolPVanimation.gif

Protocols



Gycerol stock

  • Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after
Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Glycerol_stock"