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Team:UNIPV-Pavia/Methods Materials/Glycerol stock
From 2009.igem.org
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*[[Team:UNIPV-Pavia/Methods_Materials/Digestion|BioBrick digestion with restriction enzymes]] | *[[Team:UNIPV-Pavia/Methods_Materials/Digestion|BioBrick digestion with restriction enzymes]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/GelExtraction|DNA gel extraction]] | *[[Team:UNIPV-Pavia/Methods_Materials/GelExtraction|DNA gel extraction]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]] | *[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Pcr|PCR]] | *[[Team:UNIPV-Pavia/Methods_Materials/Pcr|PCR]] | ||
<br> | <br> | ||
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'''Gycerol stock''' | '''Gycerol stock''' | ||
| + | ''(estimated time: 15 min + freezing time)'' | ||
| + | <br> | ||
| + | <br> | ||
| + | '''Materials needed:''' | ||
| + | *'''sample of overnight incubated bacteria''' | ||
| + | *'''80% glycerol''' | ||
| + | <br> | ||
*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol | *Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol | ||
*Leave at -20°C for one day | *Leave at -20°C for one day | ||
*Move to -80°C the day after | *Move to -80°C the day after | ||
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</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 15:12, 22 June 2009
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