Team:UNIPV-Pavia/Methods Materials/Glycerol stock

From 2009.igem.org

(Difference between revisions)
Line 1: Line 1:
-
{{UNIPV-Pavia/StyleCss}}
+
{{UNIPV-Pavia/Protocols}}
-
<html>
+
-
<head>
+
-
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
+
-
</head>
+
-
<body>
+
-
<table width="100%">
+
-
  <tr>
+
-
<td align="center">
+
-
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+
-
</td>
+
-
  </tr>
+
-
</table>
+
-
</body>
+
-
</html>
+
-
 
+
-
<table>
+
-
  <tr>
+
-
    <td valign="top" width= "23%">
+
-
{{UNIPV-Pavia/Menu}}
+
-
    </td>
+
-
    <td>
+
-
 
+
-
<h1>Protocols</h1>
+
-
 
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/Lb|Lb medium]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/Transformation|Transformation]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/XGal|X-Gal staining protocol for beta galactosidase (blue/white screening)]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/Glycerol stock|Glycerol stock]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction|Plasmid extraction]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/Digestion|BioBrick digestion with restriction enzymes]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/GelExtraction|DNA gel extraction]]
+
-
*[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/Pcr|PCR]]
+
-
 
+
<br>
<br>
== Gycerol stock ==  
== Gycerol stock ==  

Revision as of 15:40, 22 June 2009


Gycerol stock


(estimated time: 15 min + freezing time)'Italic text

Materials needed:

  • sample of overnight incubated bacteria
  • 80% glycerol


  • Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after 
   </td>
 </tr>

</table>

Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Glycerol_stock"