Team:Wisconsin-Madison/Cloning Protocols

From 2009.igem.org

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Insert and Vector
* Solid Culture
* Solid Culture
* Liquid Culture
* Liquid Culture
* Plasmid Isolation
* Plasmid Isolation
* Restriction Enzyme Mapping for Verification
* Restriction Enzyme Mapping for Verification
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Insert Only
* PCR to add BioBrick Restriction Cut Sites (Primer Design)
* PCR to add BioBrick Restriction Cut Sites (Primer Design)
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Insert and Vector
* PCR Plasmid Cleanup  
* PCR Plasmid Cleanup  
* Concentration Verification
* Concentration Verification

Revision as of 15:07, 18 October 2009



Overview of Cloning Protocol

Insert and Vector

  • Solid Culture
  • Liquid Culture
  • Plasmid Isolation
  • Restriction Enzyme Mapping for Verification

Insert Only

  • PCR to add BioBrick Restriction Cut Sites (Primer Design)

Insert and Vector

  • PCR Plasmid Cleanup
  • Concentration Verification
  • Digestion
  • Digestion Clean Up
  • Concentration Verification
  • Ligation
  • Transformation
  • Colony PCR Screening


Procedures for these can be found in Protocol Section
Back to Protocols
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