Team:UNIPV-Pavia/Methods Materials/Glycerol stock

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== '''Gycerol stocks''' ==  
== '''Gycerol stocks''' ==  

Revision as of 17:20, 22 June 2009

EthanolPVanimation.gif



Protocols


Gycerol stocks


(estimated time: 15 min + freezing time)

Materials needed:

  • sample of overnight incubated bacteria
  • 80% glycerol


  • Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after
Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Glycerol_stock"