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Team:Rice/Notebook
From 2009.igem.org
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| - | + | Eventually this will get formatted differently, but until then... | |
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| + | New practices for this year: | ||
| + | |||
| + | In order to better preserve our 2009 parts library, whenever you use a new BioBrick, remove the 15uL of water that you resuspended with, transfer it to a PCR tube, and store in the iGEM 2009 box at -20. Also make sure to list whatever parts you pull on this notebook page. (And, of course, make glycerol stocks of '''every''' part that you pull from the registry. | ||
| + | |||
| + | 5/23/09 | ||
| + | Thomas | ||
| + | Pulled parts: | ||
| + | (Tail switching) | ||
| + | BBa_I719005 - T7 promoter | ||
| + | BBa_I719015 - T7 promoter test circuit with GFP | ||
| + | BBa_B0034 - Strong RBS | ||
| + | BBa_B0015 - commonly used strong forward terminator | ||
| + | BBa_B1006 - new, strong forward terminator | ||
| + | (Tet selection) | ||
| + | pSB4K5 - low copy Kan vector | ||
| + | (Part for Dr. Beason) | ||
| + | BBa_J45200 | ||
| + | from the 2009 library plates. | ||
| + | |||
| + | Used 1uL of each to HS transform into XL1 competent cells. | ||
Revision as of 07:12, 25 May 2009
Eventually this will get formatted differently, but until then...
New practices for this year:
In order to better preserve our 2009 parts library, whenever you use a new BioBrick, remove the 15uL of water that you resuspended with, transfer it to a PCR tube, and store in the iGEM 2009 box at -20. Also make sure to list whatever parts you pull on this notebook page. (And, of course, make glycerol stocks of every part that you pull from the registry.
5/23/09 Thomas Pulled parts: (Tail switching) BBa_I719005 - T7 promoter BBa_I719015 - T7 promoter test circuit with GFP BBa_B0034 - Strong RBS BBa_B0015 - commonly used strong forward terminator BBa_B1006 - new, strong forward terminator (Tet selection) pSB4K5 - low copy Kan vector (Part for Dr. Beason) BBa_J45200 from the 2009 library plates.
Used 1uL of each to HS transform into XL1 competent cells.
