Team:UNIPV-Pavia/Methods Materials/Resuspension

From 2009.igem.org

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== '''LB medium''' ==
 
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''(Estimated time: 10 min + 4 hours of autoclavation and cooling)''
 
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'''Materials needed:'''
 
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*'''NaCl'''
 
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*'''BactoTryptone'''
 
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*'''Yeast extract '''
 
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*'''ddH2O'''
 
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*'''Agar'''
 
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*'''Clean bottle or E-flask'''
 
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*For a final volume of 1 l, put:
 
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**10 g NaCl
 
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**10 g BactoTryptone
 
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**5 g Yeast extract
 
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**15 g Agar (only for LB plates)
 
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*into 1 l of ddH2O.
 
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*Autoclave the solution.
 
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*Let it cool to ~40-45°C
 
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*Add the proper antibiotic if needed.
 
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*ONLY FOR PLATES:
 
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**Pour an homogenous layer of agar LB into Petri plates under the hood.
 
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**Let agar LB polymerase.
 
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*Cover and store at +4°C.
 
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*Always check for contaminations before using!
 
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== '''DNA resuspension from iGEM 2009 plates''' ==  
== '''DNA resuspension from iGEM 2009 plates''' ==  

Revision as of 17:19, 22 June 2009

EthanolPVanimation.gif



Protocols




DNA resuspension from iGEM 2009 plates

(Estimated time: 30 min)

Materials needed:

  • iGEM 2009 plates
  • PC with internet connection :-)
  • ddH20
  • 0.5 ml sterile Eppendorf tubes


  • Find the correct position of the desired DNA from the iGEM 2009 plates
  • Resuspend with ddH20 and transfer in an Eppendorf tubes
  • Store at -20°C
Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Resuspension"