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Template:UNIPV-Pavia/Protocols
From 2009.igem.org
(Difference between revisions)
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*[[Team:UNIPV-Pavia/Methods_Materials/Ligation#Ligation|Ligation]] | *[[Team:UNIPV-Pavia/Methods_Materials/Ligation#Ligation|Ligation]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/PCR#PCR|PCR]] | *[[Team:UNIPV-Pavia/Methods_Materials/PCR#PCR|PCR]] | ||
| + | *[[Team:UNIPV-Pavia/Methods_Materials/Sensing ethanol concentration#Sensing ethanol concentration|Sensing ethanol concentration: our do-it-yourself kit]] | ||
Revision as of 12:39, 17 October 2009
- LB medium
- DNA resuspension from iGEM 2009 plates
- Transformation
- X-Gal staining protocol for beta galactosidase (blue/white screening)
- Glycerol stocks
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- Electrophoresis
- DNA gel extraction
- DNA precipitation with sodium acetate
- Ligation
- PCR
- Sensing ethanol concentration: our do-it-yourself kit
