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Team:UNIPV-Pavia/Protocols/Precipitation
From 2009.igem.org
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Revision as of 15:01, 25 June 2009
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DNA precipitation with sodium acetate
(estimated time: 1 hour)
Materials needed:
- sodium acetate
- absolute ethanol
- ethanol 70%
- ddH2O
- previously cut plasmid
- We use it only if the miniprep did not give us a high yield of DNA
- Add 1/10 digestion volume of sodium acetate 3 M
- Add 2.5 digestion volume of absolute ethanol
- Freeze at -80°C for 30 min
- Centrifuge at 13000 rpm, 4°C for 20 min
- Decant supernatant
- Add 50 µl of 70% ethanol
- Centrifuge at 13000 rpm, 4°C for 20 min
- Remove all supernatant with a pipette
- Air dry pellet until ethanol is totally removed
- Elute with 5 µl ddH2O
