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Team:Kyoto/GSDD/Goals
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We designed Timer vector to constitutively express eGFP while keeping repeated sequences of LacI binding site and being stabilized. And also we designed constitutive LacI expression vector. (Totally two vectors) After complete the construction, we will move on to the functional measurement of Timer vector system; whether LacI binds to the both ends of repeated sequences and stabilized the linear Timer vector sufficiently and whether Timer vector can behave like natural linear chromosome and roughly count the replication times. (See below box) | We designed Timer vector to constitutively express eGFP while keeping repeated sequences of LacI binding site and being stabilized. And also we designed constitutive LacI expression vector. (Totally two vectors) After complete the construction, we will move on to the functional measurement of Timer vector system; whether LacI binds to the both ends of repeated sequences and stabilized the linear Timer vector sufficiently and whether Timer vector can behave like natural linear chromosome and roughly count the replication times. (See below box) | ||
| - | [[Image:figure(realgoal).png| | + | [[Image:figure(realgoal).png|700px|center|Fig.2]] |
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Revision as of 12:32, 19 October 2009
Goals
Ultimate Goal
Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times.
Real Goal
Our real goal is to suggest some possibility of realization of our ultimate goal.
We will challenge to make a “prototype” device of our ultimate goal. First time, we had planned to construct designed two vectors (Timer vector and Bomb vector) and analyze those functions. However, after considering the span of experimental period, we changed our plan and decided to concentrate on the construction and functional analysis of Timer vector system (see details in Mechanism page).
We designed Timer vector to constitutively express eGFP while keeping repeated sequences of LacI binding site and being stabilized. And also we designed constitutive LacI expression vector. (Totally two vectors) After complete the construction, we will move on to the functional measurement of Timer vector system; whether LacI binds to the both ends of repeated sequences and stabilized the linear Timer vector sufficiently and whether Timer vector can behave like natural linear chromosome and roughly count the replication times. (See below box)
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