"
Team:Team:Imperial College London/Wetlab/Protocols/Cellculture/CC11
From 2009.igem.org
(Difference between revisions)
(→SLIC) |
|||
| Line 2: | Line 2: | ||
{{Imperial/09/Tabs/Main/Wetlab/Protocols}} | {{Imperial/09/Tabs/Main/Wetlab/Protocols}} | ||
=SLIC= | =SLIC= | ||
| - | Sequence and ligation independent cloning to join multiple pieces of DNA in a single step. The procedure also takes less time than standard ligation techniques. The cloning team did, however, experience limited success with this method. | + | Sequence and ligation independent cloning (SLIC) was undertaken to join multiple pieces of DNA in a single step. The procedure also takes less time than standard ligation techniques. The cloning team did, however, experience limited success with this method. |
The In-Fusion Advantage PCR Cloning kit from Clontech was used. The protocol(in .pdf format) can be found [http://www.clontech.com/images/pt/PT4065-1.pdf here]. | The In-Fusion Advantage PCR Cloning kit from Clontech was used. The protocol(in .pdf format) can be found [http://www.clontech.com/images/pt/PT4065-1.pdf here]. | ||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 15:46, 18 October 2009
SLIC
Sequence and ligation independent cloning (SLIC) was undertaken to join multiple pieces of DNA in a single step. The procedure also takes less time than standard ligation techniques. The cloning team did, however, experience limited success with this method.
The In-Fusion Advantage PCR Cloning kit from Clontech was used. The protocol(in .pdf format) can be found [http://www.clontech.com/images/pt/PT4065-1.pdf here].
