Team:UNIPV-Pavia/Protocols/Precipitation

From 2009.igem.org

(Difference between revisions)
(DNA precipitation with sodium acetate)
Line 38: Line 38:
'''Materials needed:'''
'''Materials needed:'''
-
*'''sodium acetate'''
+
*'''Sodium acetate'''
-
*'''absolute ethanol'''
+
*'''Absolute ethanol'''
-
*'''ethanol 70%'''
+
*'''Ethanol 70%'''
*'''ddH2O'''
*'''ddH2O'''
-
*'''previously cut plasmid'''
+
*'''Previously cut plasmid'''
<br>
<br>
*We use it only if the miniprep did not give us a high yield of DNA  
*We use it only if the miniprep did not give us a high yield of DNA  

Revision as of 15:01, 25 June 2009

EthanolPVanimation.gif



Protocols

DNA precipitation with sodium acetate

(estimated time: 1 hour)

Materials needed:

  • Sodium acetate
  • Absolute ethanol
  • Ethanol 70%
  • ddH2O
  • Previously cut plasmid


  • We use it only if the miniprep did not give us a high yield of DNA
  • Add 1/10 digestion volume of sodium acetate 3 M
  • Add 2.5 digestion volume of absolute ethanol
  • Freeze at -80°C for 30 min
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Decant supernatant
  • Add 50 µl of 70% ethanol
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Remove all supernatant with a pipette
  • Air dry pellet until ethanol is totally removed
  • Elute with 5 µl ddH2O