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Team:Todai-Tokyo/Protocols/Ligation
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(New page: {{:Team:Todai-Tokyo/Template}} == Ligation Protocol == # Mix 2µl of insert DNA with 3µl of vector DNA # Add 5µl Takara Ligation High # Incubate at 17ºC for 30 min.) |
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== Ligation Protocol == | == Ligation Protocol == | ||
Latest revision as of 16:28, 20 October 2009
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
Ligation Protocol
- Mix 2µl of insert DNA with 3µl of vector DNA
- Add 5µl Takara Ligation High
- Incubate at 17ºC for 30 min.
