Team:VictoriaBC/Testing

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TESTING
+
TESTING<br>
 +
<br>
 +
<table>
 +
        <tr>
 +
            <td>Temperature</td>
 +
            <td>Room temperature</td>
 +
            <td>30&deg;C</td>
 +
            <td>34&deg;C</td>
 +
            <td>37&deg;C</td>
 +
            <td>42&deg;C</td>
 +
            <td>&nbsp;</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235032 A expected result</td>
 +
            <td>g</td>
 +
            <td>g</td>
 +
            <td>g</td>
 +
            <td>g</td>
 +
            <td>g</td>
 +
            <td>This should be repressed (limited green) without lactose or IPTG independent of temperature.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235032 A actual result</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>These were quite green.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235032 B + IPTG expected</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>This should be induced by IPTG&nbsp;and produce green independent of temperature.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235032 B +IPTG actual</td>
 +
            <td>g</td>
 +
            <td>g</td>
 +
            <td>g</td>
 +
            <td>g</td>
 +
            <td>g</td>
 +
            <td>These were green.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235033 A expected</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>Negative control to account for microscope artifacts - there is no colour producing part at all.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235033 A actual</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>This is as expected.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235036 A expected</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>R</td>
 +
            <td>R</td>
 +
            <td>R</td>
 +
            <td>This should be controlled by the 32&deg;C thermometer and red should be repressed under 32&deg;C.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235036 A actual</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>No flourescence.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235037 A expected</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>This should be controlled by the 32&deg;C thermometer and green should be repressed under 32&deg;C.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235037 A actual</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>No flourescence.&nbsp;</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235005 A expected</td>
 +
            <td>R</td>
 +
            <td>R</td>
 +
            <td>R</td>
 +
            <td>R</td>
 +
            <td>R</td>
 +
            <td>This has no promoter but might produce limited red independent of temperature.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235005 A actual</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>No flourescence.&nbsp;</td>
 +
        </tr>
 +
        <tr>
 +
            <td>J04630 expected</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>G</td>
 +
            <td>This has no promoter but might produce limited green independent of temperature.</td>
 +
        </tr>
 +
        <tr>
 +
            <td>J04630 actual</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>/</td>
 +
            <td>No flourescence.&nbsp;</td>
 +
        </tr>
</td>
</td>
</tr>
</tr>
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</table>

Revision as of 07:39, 20 October 2009


HOME
TESTING

Temperature Room temperature 30°C 34°C 37°C 42°C  
K235032 A expected result g g g g g This should be repressed (limited green) without lactose or IPTG independent of temperature.
K235032 A actual result G G G G G These were quite green.
K235032 B + IPTG expected G G G G G This should be induced by IPTG and produce green independent of temperature.
K235032 B +IPTG actual g g g g g These were green.
K235033 A expected / / / / / Negative control to account for microscope artifacts - there is no colour producing part at all.
K235033 A actual / / / / / This is as expected.
K235036 A expected / / R R R This should be controlled by the 32°C thermometer and red should be repressed under 32°C.
K235036 A actual / / / / / No flourescence.
K235037 A expected / / G G G This should be controlled by the 32°C thermometer and green should be repressed under 32°C.
K235037 A actual / / / / / No flourescence. 
K235005 A expected R R R R R This has no promoter but might produce limited red independent of temperature.
K235005 A actual / / / / / No flourescence. 
J04630 expected G G G G G This has no promoter but might produce limited green independent of temperature.
J04630 actual / / / / / No flourescence. 
PROJECT
OVERVIEW
DESIGN
TESTING
RESULTS
CONCLUSIONS
TEAM
LAB NOTEBOOK
LAB PROTOCOLS
PARTS

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