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Team:VictoriaBC/Design
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The final project consisted of a simplified biothermometer design. Using the 32°C ribothermometer linked to either RFP or GFP would entail a system that responded visually to differences in temperature. There were two levels for this: K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.<br> | The final project consisted of a simplified biothermometer design. Using the 32°C ribothermometer linked to either RFP or GFP would entail a system that responded visually to differences in temperature. There were two levels for this: K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.<br> | ||
K235034<br> | K235034<br> | ||
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<tr> | <tr> | ||
<td>K235034 (Chl) const. promoter+32oC temp. sensing RBS</td> | <td>K235034 (Chl) const. promoter+32oC temp. sensing RBS</td> | ||
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<td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td> | <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>K235032(Kan) lactose promoter+RBS+GFP+stop</td> | <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>K235005(Chl) RFP+stop</td> | <td>K235005(Chl) RFP+stop</td> | ||
<td>P1010-Kan</td> | <td>P1010-Kan</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td> | <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td> | <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td> | <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td> | ||
<td>P1010-Kan</td> | <td>P1010-Kan</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>K235031 (Chl) LacI repressor+LVA+stop</td> | <td>K235031 (Chl) LacI repressor+LVA+stop</td> | ||
<td>P1010-Kan</td> | <td>P1010-Kan</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td> | <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>K235032(Kan) lactose promoter+RBS+GFP+stop</td> | <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>K235005(Chl) RFP+stop</td> | <td>K235005(Chl) RFP+stop</td> | ||
<td>P1010-Kan</td> | <td>P1010-Kan</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td> | <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 07:34, 20 October 2009
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DESIGN The final project consisted of a simplified biothermometer design. Using the 32°C ribothermometer linked to either RFP or GFP would entail a system that responded visually to differences in temperature. There were two levels for this: K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively. K235034
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