Team:Heidelberg/Project SaO
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=='''Outlook and summary'''== | =='''Outlook and summary'''== | ||
- | :<span style="font-size:10mm;">T</span>he emergence of interest in manipulatable eukaryotic systems has posed much pressure on the development of methods to help understand and characterize eukaryotic gene regulation. Those methods go beyond the already rather sophisticated methodology still being established in prokaryotes to investigate and thereafter engineer these cells as needed [[Team:Heidelberg/Project_SaO#References|[1]]]. For one thing, the design of promoters exclusively responsive to one transcription factor (TF) within eukaryotic cells could certainly help improve our understanding of the key components of one pathway or the other, while eliminating the cross-talk often observed with many naturally occurring promoters. Such promoters have often posed a challenge to researchers studying signal transduction in eukaryotic systems because of the different types of TFs a single regulatory element can bind, and a single TF having multiple target regulatory regions [[Team:Heidelberg/Project_SaO#References|[2]]]. With the emergence of systematized research and attempts for modeling biological systems, the availability of data with minimal experimental variability and highly accurate experimental conditions has also contributed to the need for such finely-tuned promoters. Once such exclusive promoters could be available and methods for their characterization established, it is not so hard to imagine the revolutionary effect they could have on eukaryotic research. Some of many applications could be: | + | :<span style="font-size:4mm;"><span style="font-size:10mm;">T</span>he emergence of interest in manipulatable eukaryotic systems has posed much pressure on the development of methods to help understand and characterize eukaryotic gene regulation. Those methods go beyond the already rather sophisticated methodology still being established in prokaryotes to investigate and thereafter engineer these cells as needed [[Team:Heidelberg/Project_SaO#References|[1]]]. For one thing, the design of promoters exclusively responsive to one transcription factor (TF) within eukaryotic cells could certainly help improve our understanding of the key components of one pathway or the other, while eliminating the cross-talk often observed with many naturally occurring promoters. Such promoters have often posed a challenge to researchers studying signal transduction in eukaryotic systems because of the different types of TFs a single regulatory element can bind, and a single TF having multiple target regulatory regions [[Team:Heidelberg/Project_SaO#References|[2]]]. With the emergence of systematized research and attempts for modeling biological systems, the availability of data with minimal experimental variability and highly accurate experimental conditions has also contributed to the need for such finely-tuned promoters. Once such exclusive promoters could be available and methods for their characterization established, it is not so hard to imagine the revolutionary effect they could have on eukaryotic research. Some of many applications could be: |
:*Understanding disease within a network context. | :*Understanding disease within a network context. | ||
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* <!--As a further attempt, we tried to establish a [[Team:Heidelberg/stables|cell line]] that overcomes the variability in measurements caused by drawback of transient transfection by allowing the stable integration of inserts at a predetermined integration site in the genome of the cell line in use. Such an approach helps eliminating epigenetic variability in gene expression control. Although, this part was never realized in its final form, we are proud to have introduced the value of such a concept to the emerging field of eukaryotic promoter research and our own experimental observations have further strengthened our belief in the need of such a cell line in the future.--> Not only to overcome challenges for promoter measurement, but also to lay the foundations for the proposed drug-screening assay as well as any other synthetic biology idea in mammalian cells, we emphasize the importance of [[Team:Heidelberg/stables|stable cell line creation]]. | * <!--As a further attempt, we tried to establish a [[Team:Heidelberg/stables|cell line]] that overcomes the variability in measurements caused by drawback of transient transfection by allowing the stable integration of inserts at a predetermined integration site in the genome of the cell line in use. Such an approach helps eliminating epigenetic variability in gene expression control. Although, this part was never realized in its final form, we are proud to have introduced the value of such a concept to the emerging field of eukaryotic promoter research and our own experimental observations have further strengthened our belief in the need of such a cell line in the future.--> Not only to overcome challenges for promoter measurement, but also to lay the foundations for the proposed drug-screening assay as well as any other synthetic biology idea in mammalian cells, we emphasize the importance of [[Team:Heidelberg/stables|stable cell line creation]]. | ||
- | :At the end, we are proud to say that we have introduced many of the concepts, methods and tools that could serve as the basis for all other attempts in the engineering of eukaryotic gene regulatory systems. <!--Not only have we allowed the chance for many of the researchers in many biological and medical fields to enhance the selectivity of their promoters, but also helped develop the devices necessary for further characterization with the technologies available for those working in the life- and biosciences today.--> Not neglecting the need for further improvement, with such a collection of tools available the ideas of selective protein and gene therapy, metabolic engineering, stem cell manipulation and better intracellular network modeling do not seem too far away. | + | :At the end, we are proud to say that we have introduced many of the concepts, methods and tools that could serve as the basis for all other attempts in the engineering of eukaryotic gene regulatory systems. <!--Not only have we allowed the chance for many of the researchers in many biological and medical fields to enhance the selectivity of their promoters, but also helped develop the devices necessary for further characterization with the technologies available for those working in the life- and biosciences today.--> Not neglecting the need for further improvement, with such a collection of tools available the ideas of selective protein and gene therapy, metabolic engineering, stem cell manipulation and better intracellular network modeling do not seem too far away.</span> |
==References== | ==References== |
Revision as of 08:32, 20 October 2009
Outlook and summary
References
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