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Team:Todai-Tokyo/Protocols/Gel Extraction
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(New page: {{:Team:Todai-Tokyo/Template}} == Gel Purification Protocol == # Cut out volume of agarose gel containing the desired DNA and put in an eppendorf tube # Use the Promega Gel Purification...) |
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== Gel Purification Protocol == | == Gel Purification Protocol == | ||
Latest revision as of 16:30, 20 October 2009
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Gel Purification Protocol
- Cut out volume of agarose gel containing the desired DNA and put in an eppendorf tube
- Use the Promega Gel Purification kit according to instructions
- Elute DNA with 50µl of MilliQ (or 30µl if yield is low)
- Measure concentration using a spectrophotometer and write on tube
