Team:Heidelberg/Parts

From 2009.igem.org

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| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]
| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]
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| style="font-weight:bold;" |  GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]
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Revision as of 10:20, 20 October 2009


Parts

Measurement Kit

Name Part type Registry link
pSMB_MEASURE: Promoter Measurement plasmid (mammalian) Plasmid backbone [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]
pSMB_REFERENCE Plasmid [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]

Core promoters

The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.

Name Part type Registry link
JeT Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]
JeT proximal-CMV core Regulatory [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]

Promoters created by RA-PCR

All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].

Name Part type Registry link
Constitutive promoter; 0.4 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
Constitutive promoter; 1 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
Constitutive promoter; 2 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]
Constitutive promoter; 2.5 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]
NF-κB regulated promoter Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]
pPARγ regulated promoter 1 Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]
pPARγ regulated promoter 2 Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]

HEARTBEAT Predicted promoters

coming soon...

Natural promoters

Name Part type Registry link
LDL receptor promoter (sterol regulated) Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]
HMG-CoA synthase promoter (sterol regulated) Regulatory [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]
c-Jun promoter (growth factor regulated) Regulatory [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]

Targeting Signals

Protein domains for targeting to sub-cellular compartments.

Name Part type Registry link
Plasma membrane targeting (GPI anchor) Protein domain [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]
Plasma membrane targeting (myrpalm) Protein domain [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]
ER membrane targeting (C-terminal) Protein domain [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]
ER membrane targeting (N-terminal) Protein domain [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]
mCherry-myrpalm-polyA Composite [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]
GFP-polyA Composite [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]
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