Virginia Commonwealth/26 June 2009
From 2009.igem.org
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*3 Order DNA | *3 Order DNA | ||
====Wetlab==== | ====Wetlab==== | ||
- | *IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. - | + | *1 IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. - |
- | *5.0 microliters of IPTG stock was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP) | + | *2 5.0 microliters of IPTG stock was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP) |
Revision as of 15:54, 26 June 2009
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Contents |
Friday 26 June 2009
Results
- 1 Plate growing pSB3K3 plasmid grew well.
- 2 Plate growing the device plasmid pSB3K3, BBa_J23102, and E0240 grew well.
Tasks
- 1 Pick colonies and grow overnight.
- 2 Confirm the growing colonies using GFP and RFP reporter.
- 3 Order DNA
Wetlab
- 1 IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. -
- 2 5.0 microliters of IPTG stock was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP)