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Team:Virginia/29 June 2009
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(New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Discussion with Prof Kozminski **Ligation: Need to use agarose certified for in-gel ligation **Protein purification/detecti...) |
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*Cut ABP (X,P) | *Cut ABP (X,P) | ||
*Ligate @15C A09-3(3): ABP (E,P) pSB1C3 (E,P) | *Ligate @15C A09-3(3): ABP (E,P) pSB1C3 (E,P) | ||
| - | *Ligate @15C A09-5: ABP (X,P) & | + | *Ligate @15C A09-5: ABP (X,P) & J13002(S,P) |
| - | *Ligate @15C A09-6: ABP (X,P) & | + | *Ligate @15C A09-6: ABP (X,P) & J04500(S,P) |
*Starch iodine As assay - order reagents | *Starch iodine As assay - order reagents | ||
*Modeling: Installing software | *Modeling: Installing software | ||
Latest revision as of 18:32, 6 July 2009
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Overnight Results
Today
- Discussion with Prof Kozminski
- Ligation: Need to use agarose certified for in-gel ligation
- Protein purification/detection: probably want to use HA tag since we just to show we're getting protein expression; affinity purification overkill; need to consider size of tag
- Miniprep of untransformed DH5a just for control's sake
- Cut ABP (X,P)
- Ligate @15C A09-3(3): ABP (E,P) pSB1C3 (E,P)
- Ligate @15C A09-5: ABP (X,P) & J13002(S,P)
- Ligate @15C A09-6: ABP (X,P) & J04500(S,P)
- Starch iodine As assay - order reagents
- Modeling: Installing software
