Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
(Difference between revisions)
(→September 25, 2009) |
(→September 25, 2009) |
||
Line 602: | Line 602: | ||
'''iptg+cox+ogr+ter''' | '''iptg+cox+ogr+ter''' | ||
+ | |||
+ | The second lane sample was selected for ligation with IPTG | ||
+ | |||
+ | Ligation Reaction: | ||
+ | |||
+ | T4 ligase 1µL | ||
+ | Buffer 2µL | ||
+ | cox+ogr+ter 5µL | ||
+ | iptg 5µL | ||
+ | Water 10µL | ||
+ | Total 20µL | ||
+ | |||
+ | DH5alpha competent cells were transformed and platted over LB Amp100 and incubate at 37ºC | ||
+ | |||
+ | |||
+ | |||
Revision as of 20:47, 20 October 2009