Team:British Columbia

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(The Bacterial Traffic Light)
(The Bacterial Traffic Light)
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<img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="trafficlight">
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<map name="trafficlight">
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<area shape="circle" coords="774,121,54" alt="Sensors: This links to the pBAD promoters that sense the arabinose." href="green" />
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<area shape="circle" coords="775,236,55" alt="Lock and Key: this controls when the colors are produced" href="yellow" />
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<area shape="circle" coords="774,350,54" alt="The Jammer: this mRNA sequence blocks the key from opening the lock." href="red" />
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[[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]]
 
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[[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]]
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The Traffic Light is composed of three distinct subparts:
The Traffic Light is composed of three distinct subparts:

Revision as of 05:28, 21 October 2009


The Bacterial Traffic Light

The goal of our project is to construct an Eschericia coli bacterial "traffic light", that responds to increasing concentration levels of a given input (in our case, arabinose) with green, yellow, and red fluorescent outputs. Our project is unique because it is an analog biosensor, capable of responding to a continuous variable signal, rather than a binary biosensor that only signals on/off.

Sensors: This links to the pBAD promoters that sense the arabinose. Lock and Key: this controls when the colors are produced The Jammer: this mRNA sequence blocks the key from opening the lock.


The Traffic Light is composed of three distinct subparts: