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Team:Imperial College London/Wetlab/Results/Thermoinduction
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Understanding of the fluorescence data requires normalization with cell growth data, in the form of optical density (absorbance). | Understanding of the fluorescence data requires normalization with cell growth data, in the form of optical density (absorbance). | ||
==28 degrees Celsius== | ==28 degrees Celsius== | ||
| - | [[Image:II09_28degs.jpg]] | + | [[Image:II09_28degs.jpg]]<br> |
| + | <b>Figure 1: Absorbance at 28 degrees Celsius </b> | ||
==42 degrees Celsius== | ==42 degrees Celsius== | ||
[[Image:II09_42deg.jpg]] | [[Image:II09_42deg.jpg]] | ||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 22:44, 20 October 2009
Contents |
Experiment Rationale
To investigate the behaviour of the lamda-cI thermoinducible construct and show that when temperature is low (at 28 degrees Celsius), there is low fluorescence output. This shows that the genome deletion module is repressed. When temperature is raised to 42 degrees Celsius, fluorescence increases, indicating that the repression is lifted. We are looking at both absorbance and fluorescence data.
Absorbance data
Understanding of the fluorescence data requires normalization with cell growth data, in the form of optical density (absorbance).
28 degrees Celsius
Figure 1: Absorbance at 28 degrees Celsius
42 degrees Celsius
