Team:UNIPV-Pavia/Methods Materials/Glycerol stock
From 2009.igem.org
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(→Glycerol stocks) |
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<table width='80%'><tr><td> | <table width='80%'><tr><td> | ||
- | *Mix 750 ul from the 5 ml sample of | + | *Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol |
*Leave at -20°C for one day | *Leave at -20°C for one day | ||
*Move to -80°C the day after | *Move to -80°C the day after | ||
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<table border='1'> | <table border='1'> | ||
<tr><td> | <tr><td> | ||
- | *'''o/n | + | *'''o/n or in exp phase bacterial culture''' |
*'''80% glycerol''' | *'''80% glycerol''' | ||
</td></tr></table></div><br><br><br><br> | </td></tr></table></div><br><br><br><br> | ||
</td></tr></table> | </td></tr></table> |
Revision as of 19:48, 1 July 2009
Glycerol stocks
(estimated time: 15 min + freezing time)
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