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Revision as of 06:51, 8 September 2009
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Protocols
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LB medium
(Estimated time: 10 min + 4 hours of autoclavation and cooling)
- For a final volume of 1 l, put:
- 10 g NaCl
- 10 g BactoTryptone
- 5 g Yeast extract
- 15 g Agar (only for LB plates)
- into 1 l of ddH2O.
- Autoclave the solution.
- Let it cool to ~40-45°C
- Add the proper antibiotic if needed.
- ONLY FOR PLATES:
- Pour an homogenous layer of agar LB into Petri plates under the hood.
- Let agar LB polymerase.
- Cover and store at +4°C.
- Always check for contaminations before using!
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Clean bottle or flask
NaCl
BactoTryptone
Yeast extract
ddH2O
Agar
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"
