Team:British Columbia
From 2009.igem.org
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- | <img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="trafficlight"> | + | |
+ | <img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="#trafficlight"> | ||
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<map name="trafficlight"> | <map name="trafficlight"> | ||
- | <area shape="circle" coords="774,121,54" alt="Sensors: This links to the pBAD promoters that sense the arabinose." href="/Team:British_Columbia/pBAD" /> | + | <area shape="circle" |
- | <area shape="circle" coords="775,236,55" alt="Lock and Key: this controls when the colors are produced" href="/Team:British_Columbia/LockandKey" /> | + | coords="774,121,54" |
- | <area shape="circle" coords="774,350,54" alt="The Jammer: this mRNA sequence blocks the key from opening the lock." href="/Team:British_Columbia/Jammer" /> | + | alt="Sensors: This links to the pBAD promoters that sense the arabinose." |
+ | title="Sensors: This links to the pBAD promoters that sense the arabinose." | ||
+ | href="/Team:British_Columbia/pBAD" | ||
+ | /> | ||
+ | |||
+ | <area shape="circle" | ||
+ | coords="775,236,55" | ||
+ | alt="Lock and Key: this controls when the colors are produced" | ||
+ | title="Lock and Key: this controls when the colors are produced" | ||
+ | href="/Team:British_Columbia/LockandKey" | ||
+ | /> | ||
+ | |||
+ | <area shape="circle" | ||
+ | coords="774,350,54" | ||
+ | alt="The Jammer: this mRNA sequence blocks the key from opening the lock." | ||
+ | title="The Jammer: this mRNA sequence blocks the key from opening the lock." | ||
+ | href="/Team:British_Columbia/Jammer" | ||
+ | /> | ||
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Revision as of 05:36, 21 October 2009
[http://www.ubc.ca ]
[http://www.ubc.ca ]
Home Team Traffic Light Sensor Lock&Key Jammer [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=British_Columbia Parts] Safety Sponsors Notebook Bibliography
The Bacterial Traffic Light
The goal of our project is to construct an Eschericia coli bacterial "traffic light", that responds to increasing concentration levels of a given input (in our case, arabinose) with green, yellow, and red fluorescent outputs. Our project is unique because it is an analog biosensor, capable of responding to a continuous variable signal, rather than a binary biosensor that only signals on/off.
The Traffic Light is composed of three distinct subparts:
- The pBAD promoter family
- The lock and key riboregulation system
- The Jammer.