Team:IIT Bombay India/Notebook
From 2009.igem.org
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[[Week1]] FACS Trial | [[Week1]] FACS Trial | ||
- | [[Week2]] | + | [[Week2]] (May, 18-23) Preparation |
- | [[Week3]] | + | [[Week3]] (May, 25-30) FACS |
- | [[Week4]] | + | [[Week4]] (Jun, 1-6) Growth Curve |
- | [[Week5]] | + | [[Week5]] (Jun, 8-13) FACS:YFP signal |
- | [[Week6]] | + | [[Week6]] (Jun, 15-20) FACS:CFP signal |
- | [[Week7]] | + | [[Week7]] (Jun, 22-27) FACS repeat |
- | [[Week8]] | + | [[Week8]] (Jun-Jul, 29-4)Req.:Transformation |
- | [[Week9]] | + | [[Week9]] (Jul, 6-11) Cultured Host |
- | [[Week10]] | + | [[Week10]](Jul, 13-18) Transformation of 4 strains |
- | [[Week11]] | + | [[Week11]](Jul, 20-24) FACS:Constructs |
- | [[Week12]] | + | [[Week12]](Jul, 27-31) FACS |
- | [[Week13]] Transformations in ''E.coli'' MG1655 (lac I deleted | + | [[Week13]](Aug, 3-8) Transformations in ''E.coli'' MG1655 (lac I deleted) |
- | [[Week14]] | + | [[Week14]](Aug, 10-15) Preparation for time course expression analysis |
- | [[Week15]] | + | [[Week15]](Aug, 17-22) FACS time course |
- | [[Week16]] | + | [[Week16]](Aug, 24-29) FACS: different IPTG conc. |
- | [[Week17]] | + | [[Week17]](Aug-Sep, 31-5)Growth curves for all the four strains |
- | [[Week18]] | + | [[Week18]](Sep, 7-13) Plasmid extraction and Gel electrophoresis |
- | [[Week19]] Growth curve on Lactose (ptet A and plac K | + | [[Week19]](Sep, 14-20) Growth curve on Lactose (ptet A and plac K) |
- | [[Week20]] Growth curve on Lactose (plac A and ptet K | + | [[Week20]](Sep, 21-27) Growth curve on Lactose (plac A and ptet K) |
- | [[Week21]] Growth curve on Lactose varying IPTG concentrations (ptet A and plac K | + | [[Week21]](Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K) |
- | [[Week22]] Growth curve on Lactose varying IPTG concentrations (plac A and ptet K | + | [[Week22]](Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K) |
- | [[Week23]] Growth curve on Lactose (ptet A and plac K | + | [[Week23]](Oct, 12-17)Growth curve on Lactose (ptet A and plac K) |
- | [[Week24]] | + | [[Week24]](Oct, 18-21)Data compilation and analysis, wiki uploads |
Revision as of 08:43, 21 October 2009
Week1 FACS Trial
Week2 (May, 18-23) Preparation
Week3 (May, 25-30) FACS
Week4 (Jun, 1-6) Growth Curve
Week5 (Jun, 8-13) FACS:YFP signal
Week6 (Jun, 15-20) FACS:CFP signal
Week7 (Jun, 22-27) FACS repeat
Week8 (Jun-Jul, 29-4)Req.:Transformation
Week9 (Jul, 6-11) Cultured Host
Week10(Jul, 13-18) Transformation of 4 strains
Week11(Jul, 20-24) FACS:Constructs
Week12(Jul, 27-31) FACS
Week13(Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)
Week14(Aug, 10-15) Preparation for time course expression analysis
Week15(Aug, 17-22) FACS time course
Week16(Aug, 24-29) FACS: different IPTG conc.
Week17(Aug-Sep, 31-5)Growth curves for all the four strains
Week18(Sep, 7-13) Plasmid extraction and Gel electrophoresis
Week19(Sep, 14-20) Growth curve on Lactose (ptet A and plac K)
Week20(Sep, 21-27) Growth curve on Lactose (plac A and ptet K)
Week21(Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
Week22(Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
Week23(Oct, 12-17)Growth curve on Lactose (ptet A and plac K)
Week24(Oct, 18-21)Data compilation and analysis, wiki uploads