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Virginia Commonwealth/30 June 2009
From 2009.igem.org
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*Retransform cells | *Retransform cells | ||
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====Wetlab==== | ====Wetlab==== | ||
*Cells were retransformed with DNA from BioBrick stock and e. coli cells DEB 3.1 | *Cells were retransformed with DNA from BioBrick stock and e. coli cells DEB 3.1 | ||
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*For the e. coli that was not zapped, 35 microliters was taken directly from the cell stock and placed on a Kan plate, and 35 microliters was placed on an LB plate | *For the e. coli that was not zapped, 35 microliters was taken directly from the cell stock and placed on a Kan plate, and 35 microliters was placed on an LB plate | ||
*Cells will be incubated overnight | *Cells will be incubated overnight | ||
| + | [[User:Trentay|Trentay]] 21:26, 30 June 2009 (UTC) | ||
Revision as of 21:26, 30 June 2009
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Contents |
Tuesday 30 June 2009
Results
- E. coli cells Deb 3.1 with the plasmid backbone psB3k3 p1010 (death gene) had no visible colonies
- Negative (-) Control of the Deb 3.1 on Kan has small colonies visible on it
- other Negative (-) and positive (+) controls on LB plates had a lot of colonies.
- Possible reasons for lack of growth on Kan plate
- Kan plates were incubated at a 47 degrees Celsius (not 37) which may have caused a problem
- Possible mislabeling of plates (negative kan control and plasmid plate)
- Waiting too long to add the LB broth after zapping the cells
- Possible reasons for lack of growth on Kan plate
Tasks
- Retransform cells
Wetlab
- Cells were retransformed with DNA from BioBrick stock and e. coli cells DEB 3.1
- The controls for the experiment are:
- E. Coli Cells on LB (+)
- E. Coli Cells on LB with KAN (-)
- E. Coli Cells Zapped on LB (+)
- Care was taken to keep everything chilled and to immediately add LB broth after zapping cells.
- For the e. coli that was not zapped, 35 microliters was taken directly from the cell stock and placed on a Kan plate, and 35 microliters was placed on an LB plate
- Cells will be incubated overnight
Trentay 21:26, 30 June 2009 (UTC)
