Team:IIT Bombay India/Notebook

From 2009.igem.org

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[[Week1]] FACS Trial
[[Week1]] FACS Trial
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[[Week2]] (May, 18-23)   Preparation  
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[[Week2]] (May, 18-23)     Preparation  
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[[Week3]] (May, 25-30)   FACS  
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[[Week3]] (May, 25-30)     FACS  
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[[Week4]] (Jun, 1-6)     Growth Curve  
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[[Week4]] (Jun, 1-6)       Growth Curve  
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[[Week5]] (Jun, 8-13)   FACS:YFP signal  
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[[Week5]] (Jun, 8-13)     FACS:YFP signal  
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[[Week6]] (Jun, 15-20)   FACS:CFP signal  
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[[Week6]] (Jun, 15-20)     FACS:CFP signal  
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[[Week7]] (Jun, 22-27)   FACS repeat  
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[[Week7]] (Jun, 22-27)     FACS repeat  
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[[Week8]] (Jun-Jul, 29-4)Req.:Transformation  
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[[Week8]] (Jun-Jul, 29-4) Req.:Transformation  
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[[Week9]] (Jul, 6-11)   Cultured Host  
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[[Week9]] (Jul, 6-11)     Cultured Host  
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[[Week10]](Jul, 13-18)   Transformation of 4 strains  
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[[Week10]] (Jul, 13-18)     Transformation of 4 strains  
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[[Week11]](Jul, 20-24)   FACS:Constructs  
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[[Week11]] (Jul, 20-24)     FACS:Constructs  
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[[Week12]](Jul, 27-31)   FACS  
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[[Week12]] (Jul, 27-31)     FACS  
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[[Week13]](Aug, 3-8)     Transformations in ''E.coli'' MG1655 (lac I deleted)  
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[[Week13]] (Aug, 3-8)       Transformations in ''E.coli'' MG1655 (lac I deleted)  
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[[Week14]](Aug, 10-15)   Preparation for time course expression analysis  
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[[Week14]] (Aug, 10-15)     Preparation for time course expression analysis  
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[[Week15]](Aug, 17-22)   FACS time course  
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[[Week15]] (Aug, 17-22)     FACS time course  
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[[Week16]](Aug, 24-29)   FACS: different IPTG conc.  
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[[Week16]] (Aug, 24-29)     FACS: different IPTG conc.  
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[[Week17]](Aug-Sep, 31-5)Growth curves for all the four strains  
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[[Week17]] (Aug-Sep, 31-5) Growth curves for all the four strains  
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[[Week18]](Sep, 7-13)   Plasmid extraction and Gel electrophoresis  
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[[Week18]] (Sep, 7-13)     Plasmid extraction and Gel electrophoresis  
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[[Week19]](Sep, 14-20)   Growth curve on Lactose (ptet A and plac K)  
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[[Week19]] (Sep, 14-20)     Growth curve on Lactose (ptet A and plac K)  
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[[Week20]](Sep, 21-27)   Growth curve on Lactose (plac A and ptet K)  
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[[Week20]] (Sep, 21-27)     Growth curve on Lactose (plac A and ptet K)  
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[[Week21]](Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
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[[Week21]] (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
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[[Week22]](Oct, 5-10)   Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
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[[Week22]] (Oct, 5-10)     Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
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[[Week23]](Oct, 12-17)Growth curve on Lactose (ptet A and plac K)  
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[[Week23]] (Oct, 12-17)     Growth curve on Lactose (ptet A and plac K)  
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[[Week24]](Oct, 18-21)Data compilation and analysis, wiki uploads
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[[Week24]] (Oct, 18-21)     Data compilation and analysis, wiki uploads

Revision as of 08:46, 21 October 2009

Week1 FACS Trial

Week2 (May, 18-23) Preparation

Week3 (May, 25-30) FACS

Week4 (Jun, 1-6) Growth Curve

Week5 (Jun, 8-13) FACS:YFP signal

Week6 (Jun, 15-20) FACS:CFP signal

Week7 (Jun, 22-27) FACS repeat

Week8 (Jun-Jul, 29-4) Req.:Transformation

Week9 (Jul, 6-11) Cultured Host

Week10 (Jul, 13-18) Transformation of 4 strains

Week11 (Jul, 20-24) FACS:Constructs

Week12 (Jul, 27-31) FACS

Week13 (Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)

Week14 (Aug, 10-15) Preparation for time course expression analysis

Week15 (Aug, 17-22) FACS time course

Week16 (Aug, 24-29) FACS: different IPTG conc.

Week17 (Aug-Sep, 31-5) Growth curves for all the four strains

Week18 (Sep, 7-13) Plasmid extraction and Gel electrophoresis

Week19 (Sep, 14-20) Growth curve on Lactose (ptet A and plac K)

Week20 (Sep, 21-27) Growth curve on Lactose (plac A and ptet K)

Week21 (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)

Week22 (Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)

Week23 (Oct, 12-17) Growth curve on Lactose (ptet A and plac K)

Week24 (Oct, 18-21) Data compilation and analysis, wiki uploads