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Team:Alberta/Project/AnchTermAnneal
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To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp. | To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp. | ||
| + | 1) Combine 10 μL of Anchor/Term_(A or B) and Anchor/Term_Comp 100 μM stock solutions into an eppendorf tube. | ||
| + | 2) Add 80 μL TE, mix. | ||
| + | 3) Heat solution to 95C for 2 minutes. | ||
| + | 4) Let cool to room temperature slowly. | ||
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Revision as of 16:49, 21 October 2009
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Annealing Anchor and Terminator BytesInstructions on how to anneal the complementary strands to obtain the same concentrations we used in our experiments (10μM).What you will need:
Procedure:To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp. 1) Combine 10 μL of Anchor/Term_(A or B) and Anchor/Term_Comp 100 μM stock solutions into an eppendorf tube. 2) Add 80 μL TE, mix. 3) Heat solution to 95C for 2 minutes. 4) Let cool to room temperature slowly. |
