Team:TUDelft/Project
From 2009.igem.org
(Difference between revisions)
(→Project Overview) |
(→Self-destructive plasmid) |
||
Line 8: | Line 8: | ||
===Self-destructive plasmid=== | ===Self-destructive plasmid=== | ||
[[Image:TUDelft_logo.png|50px|right|frame]] | [[Image:TUDelft_logo.png|50px|right|frame]] | ||
- | Research question: | + | '''Research question:''' |
Can we achieve cell-to-cell communication: | Can we achieve cell-to-cell communication: | ||
#Using delay device | #Using delay device | ||
#Using self-destructive plasmid | #Using self-destructive plasmid | ||
#Between two F+ cells | #Between two F+ cells | ||
- | Goals: | + | '''Goals:''' |
<br> | <br> | ||
- | * | + | *Build a delay device that can change the timing of transcribing the target protein |
- | + | *Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself) | |
- | * | + | *Construct a modified F plasmid that will allow F+/F+ conjugation |
- | * | + | |
- | + | ||
<br> | <br> | ||
- | Why? | + | '''Why?''' |
<br> | <br> | ||
*To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics | *To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics | ||
Line 28: | Line 26: | ||
*Because it is cool!!! | *Because it is cool!!! | ||
<br><br> | <br><br> | ||
- | How? | + | '''How?''' |
<br> | <br> | ||
Not known yet :D | Not known yet :D | ||
<br><br> | <br><br> | ||
- | Applications: | + | '''Applications:''' |
<br> | <br> | ||
*Cell synchronization | *Cell synchronization |
Revision as of 20:18, 3 July 2009
Page is under construction
Project Overview
Our abstract
Finally TUDelft team has a project!!!
Self-destructive plasmid
Research question: Can we achieve cell-to-cell communication:
- Using delay device
- Using self-destructive plasmid
- Between two F+ cells
Goals:
- Build a delay device that can change the timing of transcribing the target protein
- Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
- Construct a modified F plasmid that will allow F+/F+ conjugation
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
- To generate a pulse in the expression of a certain gene
- To control the amplitude of the pulse created by the plasmid
- Because it is cool!!!
How?
Not known yet :D
Applications:
- Cell synchronization
- Selectable marker recycling
- Metabolic control analysis
- Recombination
- …
Project Details
The Experiments
Results