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| Line 302: |
Line 302: |
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| | | | |
| - | == September ==
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| | | | |
| - | ===September 3===
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| - |
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| - | '''Roxanne, Ashley, Kirsten'''
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| - |
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| - | Made 1.5L agar LB media according to lab protocol. Roxanne is autoclaving tomorrow.
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| - |
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| - | Pick 3 lumazine-dT colonies from glycerol stocks to grow overnight
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| - |
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| - | Restriction Digest
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| - |
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| - | Reaction 1
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| - |
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| - | {|
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| - | |
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| - | |'''Ribo-cheZ-dT'''
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| - | |''GFP-dT''
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| - | |sRBS-mmS6
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| - | |'''Mr. Gene mmS6'''
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| - | |-
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| - | |milliQ water
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| - | |'''5 µL'''
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| - | |''5 µL''
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| - | |5 µL
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| - | |'''5 µL'''
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| - | |-
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| - | |Buffer tango
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| - | |'''3 µL'''
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| - | |''3 µL''
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| - | |3 µL
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| - | |'''3 µL'''
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| - | |-
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| - | |DNA
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| - | |'''20 µL'''
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| - | |''20 µL''
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| - | |20 µL
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| - | |'''20 µL'''
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| - | |-
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| - | |XbaI
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| - | |'''1 µL'''
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| - | |''1 µL''
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| - | |1 µL
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| - | |'''1 µL'''
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| - | |-
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| - | |PstI
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| - | |'''1 µL'''
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| - | |''1 µL''
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| - | |1 µL
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| - | |'''1 µL'''
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| - | |-
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| - | |total
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| - | |'''30 µL'''
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| - | |''30 µL''
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| - | |30 µL
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| - | |'''30 µL'''
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| - | |}
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| - |
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| - | Reaction 2
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| - |
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| - |
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| - | {|
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| - | |
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| - | |'''Ribo-cheZ-dT'''
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| - | |''GFP-dT''
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| - | |sRBS-mmS6
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| - | |'''Mr. Gene mmS6'''
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| - | |-
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| - | |milliQ water
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| - | |'''3 µL'''
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| - | |''3 µL''
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| - | |3 µL
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| - | |'''3 µL'''
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| - | |-
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| - | |Buffer tango
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| - | |'''1 µL'''
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| - | |''1 µL''
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| - | |1 µL
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| - | |'''1 µL'''
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| - | |-
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| - | |DNA
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| - | |'''5 µL'''
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| - | |''5 µL''
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| - | |5 µL
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| - | |'''5 µL'''
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| - | |-
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| - | |EcoRI
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| - | |'''1 µL'''
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| - | |''1 µL''
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| - | |1 µL
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| - | |'''1 µL'''
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| - | |-
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| - | |total
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| - | |'''10 µL'''
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| - | |''10 µL''
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| - | |10 µL
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| - | |'''10 µL'''
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| - | |}
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| - |
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| - | Into HWB at 37°C for 2 hours
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| - |
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| - | Gel Electrophoresis (concentration):
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| - |
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| - | Lane
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| - | # 1kb ladder
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| - | # ribo-cheZ-dT (EcoRI)
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| - | #GFP-dT (EcoRI)
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| - | #sRBS-mmS6-dT (EcoRI)
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| - | #NEYFP (XbaI,PstI)
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| - | #NEYFP (ecoRI, speI)
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| - | #NCFP (ecoRI, speI)
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| - | #NECFP (xbaI,pstI)
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| - | #CCFP(ecoRI,speI)
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| - | #CECFP(ecoRI,speI)
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| - | #CCFP (notI,pstI)
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| - | #NEYFP (ecoRI,speI)
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| - | #CECFP (xbaI)
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| - | #CEYFP(xbaI,pstI)
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| - | #NCFP (xbaI,pstI)
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| - | #CEYFP(xbaI)
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| - | #pLacI
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| - | #pStrong
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| - | #1kb ladder
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| - | 5µL sample, 1 µL loading dye (6x)
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| - | 6 µL ladder
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| - |
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| - | [[Image:September_3_concentration_gel.png|250px]]
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| - |
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| - | Ran at 100V for 1 hour
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| - |
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| - | Concentrations good!
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| - |
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| - | '''Alix'''
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| - |
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| - | Gel extraction of:
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| - |
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| - | sRBS-mmS6-dT
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| - |
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| - | riboswitch-cheZ-dT
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| - |
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| - | GFP-dT
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| - |
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| - | mmS6
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| - |
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| - | Following Qiagen benchtop protocol
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| - |
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| - | ===September 4===
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| - |
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| - | '''Ashley'''
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| - |
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| - | Ligation of:
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| - |
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| - | *C-term-dT +C-CFP #2
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| - | *C-term-dT +C-ECFP #2
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| - | *C-term-dT + C-EYFP #2
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| - | *pSB1A3-2+C-CFP#1
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| - | *pSB1A3-2+C-ECFP#1
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| - | *pSB1A3-2+C-EYFP#1
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| - | *mRBS-N-term +N-CFP#1
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| - | *mRBS-N-term +N-EYFP#1
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| - | *mRBS-N-term +N-ECFP#1
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| - | *pSB1A3-1+N-CFP#2
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| - | *pSB1A3-1+N-ECFP#2
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| - | *pSB1A3-1+N-EYFP#2
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| - |
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| - | Reaction
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| - |
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| - | {|
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| - | |
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| - | |'''Control'''
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| - | |All reactions
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| - | |-
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| - | |milliQ water
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| - | |'''7.5 µL'''
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| - | |4.5 µL
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| - | |-
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| - | |10x buffer T4
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| - | |'''1 µL'''
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| - | |1 µL
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| - | |-
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| - | |Vector
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| - | |'''1 µL'''
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| - | |1 µL
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| - | |-
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| - | |Insert
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| - | |'''0 µL'''
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| - | |3 µL
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| - | |-
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| - | |T4 DNA ligase
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| - | |'''0.5 µL'''
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| - | |0.5 µL
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| - | |-
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| - | |total
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| - | |'''10 µL'''
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| - | |10 µL
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| - | |}
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| - |
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| - | Restriction Digest
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| - |
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| - | {|
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| - | |
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| - | |Control (no enzyme)
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| - | |'''Lumazine-dT (1)'''
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| - | |-
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| - | |milliQ water
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| - | |2.4 µL
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| - | |'''0.4 µL'''
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| - | |-
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| - | |10x buffer Tango
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| - | |1.6 µL
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| - | |'''1.6 µL'''
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| - | |-
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| - | |DNA
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| - | |16 µL
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| - | |'''16 µL'''
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| - | |-
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| - | |XbaI
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| - | |0 µL
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| - | |'''1 µL'''
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| - | |-
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| - | |PstI
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| - | |0 µL
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| - | |'''1 µL'''
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| - | |-
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| - | |total
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| - | |20 µL
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| - | |'''20 µL'''
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| - | |}
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| - |
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - | <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
| |
| - | <style type="text/css">
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| - |
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| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
| |
| - | .qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
| |
| - |
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| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
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| - |
| |
| - |
| |
| - | /* QuickMenu 0 */
| |
| - |
| |
| - | /*"""""""" (MAIN) Container""""""""*/
| |
| - | #qm0
| |
| - | {
| |
| - | width:auto;
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| - | background-color:transparent;
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| - | }
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| - |
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| - |
| |
| - | /*"""""""" (MAIN) Items""""""""*/
| |
| - | #qm0 a
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| - | {
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| - | padding:5px 60px 3px 0px;
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| - | margin:0px 5px 0px 0px;
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| - | color:#FFD600;
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| - | font-family:Arial;
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| - | font-size:12px;
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| - | text-decoration:none;
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| - | font-weight:bold;
| |
| - | border-width:0px 0px 8px 0px;
| |
| - | border-style:solid;
| |
| - | border-color:#FFD600;
| |
| - | }
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| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State""""""""*/
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| - | #qm0 a:hover
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| - | {
| |
| - | color:#3C13AF;
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| - | border-color:#FFAD00;
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| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
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| - | #qm0 li:hover>a
| |
| - | {
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| - | color:#3C13AF;
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| - | border-color:#FFAD00;
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| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Parent Items""""""""*/
| |
| - | #qm0 .qmparent
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| - | {
| |
| - | background-repeat:no-repeat;
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| - | background-position:95% 50%;
| |
| - | }
| |
| - |
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| - |
| |
| - | /*"""""""" (MAIN) Active State""""""""*/
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| - | body #qm0 .qmactive, body #qm0 .qmactive:hover
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| - | {
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| - | color:#3C13AF;
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| - | text-decoration:none;
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| - | border-color:#FFAD00;
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| - | }
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| - |
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| - |
| |
| - | /*"""""""" (SUB) Container""""""""*/
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| - | #qm0 div, #qm0 ul
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| - | {
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| - | padding:10px 5px 10px 5px;
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| - | background-color:#EEEEEE;
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| - | border-width:1px;
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| - | border-style:solid;
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| - | border-color:#3C13AF;
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| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Items""""""""*/
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| - | #qm0 div a, #qm0 ul a
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| - | {
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| - | padding:3px 5px 3px 5px;
| |
| - | background-color:transparent;
| |
| - | color:#230672;
| |
| - | font-size:11px;
| |
| - | font-weight:normal;
| |
| - | border-width:0px;
| |
| - | border-style:none;
| |
| - | border-color:#230672;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State""""""""*/
| |
| - | #qm0 div a:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 ul li:hover>a
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Parent Items""""""""*/
| |
| - | #qm0 div .qmparent, #qm0 ul .qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_0.gif);
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| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Active State""""""""*/
| |
| - | body #qm0 div .qmactive, body #qm0 div .qmactive:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | color:#FFFFFF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Titles""""""""*/
| |
| - | #qm0 .qmtitle
| |
| - | {
| |
| - | margin:2px 5px 5px 5px;
| |
| - | color:#222222;
| |
| - | font-family:Arial;
| |
| - | font-size:11px;
| |
| - | font-weight:bold;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Horizontal Dividers""""""""*/
| |
| - | #qm0 .qmdividerx
| |
| - | {
| |
| - | border-top-width:1px;
| |
| - | margin:4px 5px 4px 5px;
| |
| - | border-color:#999999;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 ul
| |
| - | {
| |
| - | width:100%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 li:hover > a.qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | </style>
| |
| - |
| |
| - | <!-- Add-On Core Code (Remove when not using any add-on's) -->
| |
| - | <style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
| |
| - |
| |
| - | <!-- Add-On Settings -->
| |
| - | <script type="text/JavaScript">
| |
| - |
| |
| - | /******* Menu 0 Add-On Settings *******/
| |
| - | var a = qmad.qm0 = new Object();
| |
| - |
| |
| - | // Match Widths Add On
| |
| - | a.mwidths_active = true;
| |
| - |
| |
| - | // IE Over Select Fix Add On
| |
| - | a.overselects_active = true;
| |
| - |
| |
| - | </script>
| |
| - |
| |
| - | <!-- Core QuickMenu Code -->
| |
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| - |
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| - | <!-- Add-On Code: Match Widths -->
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| - | <script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
| |
| - |
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| - | <!-- Add-On Code: IE Over Select Fix -->
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| - | <script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
| |
| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
| |
| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
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| - | <li class="qmclear"> </li></ul>
| |
| - |
| |
| - | <!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
| |
| - | <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
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| - | </body>
| |
| - | </html>
| |
| - |
| |
| - | ===September 8===
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| - |
| |
| - | '''Ashley'''
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| - |
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| - | Made 1.5L LB agar according to protocol
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| - |
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| - | Made 300mL LB broth according to protocol
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| - |
| |
| - | Gel Electrophoresis (for extraction)
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| - |
| |
| - | 5 µL ladder
| |
| - |
| |
| - | 5 µL sample, 1 µL dye
| |
| - |
| |
| - | Lane
| |
| - | # 1 kb ladder
| |
| - | #empty^
| |
| - | #empty
| |
| - | # lumazine-dT (XbaI, PstI) 600bp
| |
| - |
| |
| - | ^there was a control (no enzyme) but not enough DNA to load
| |
| - |
| |
| - | Ran at 1000V for one hour
| |
| - |
| |
| - | Gel extraction according to Qiagen benchtop protocol
| |
| - |
| |
| - |
| |
| - |
| |
| - | ===Sept 10===
| |
| - |
| |
| - | '''Ashley, Kirsten'''
| |
| - |
| |
| - | Ran 1% agarose
| |
| - |
| |
| - | Lane
| |
| - |
| |
| - | # 1 kb ladder
| |
| - | #C-term-dT + C-EYFP #2
| |
| - | #pSB1A3-1+N-CFP#2
| |
| - | #pSB1A3-1+N-ECFP#2
| |
| - | #lumazine+sRBS
| |
| - | #pSB1A3-2+C-EYFP#1
| |
| - | #pSB1A3-1+N-EYFP#2
| |
| - | #mRBS-N-term +N-CFP#1
| |
| - | #mmS6+pSB1A3-1
| |
| - | #GFP-dT
| |
| - | #mmS6-dT
| |
| - | #pSB1A3-2+C-ECFP#1
| |
| - | #mRBS-N-term +N-ECFP#1
| |
| - | #mRBS-N-term +N-EYFP#1
| |
| - | #C-term-dT +C-CFP #2
| |
| - | #C-term-dT +C-ECFP #2
| |
| - | #pSB1A3-2+C-CFP#1
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| - |
| |
| - | 3 µL sample + 0.5 µL dye
| |
| - |
| |
| - | 6 µL 1 kb ladder
| |
| - |
| |
| - | '''Mackenzie'''
| |
| - |
| |
| - | Transformation into DH5α
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| - |
| |
| - | *sRBS-lumazine-dT (km)
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| - | *mmS6-dT (km)
| |
| - | *pSB1A3+mmS6
| |
| - | *mRBS-N-term +N-ECFP#1
| |
| - | *mRBS-N-term +N-EYFP#1
| |
| - | *C-term-dT +C-ECFP #2
| |
| - | *C-term-dT + C-EYFP #2
| |
| - | *pSB1A3-2+C-ECFP#1
| |
| - | *pSB1A3-2+C-EYFP#1
| |
| - |
| |
| - | The following were not transformed due to lack of competent cells:
| |
| - |
| |
| - | *pSB1A3-1+N-ECFP#2
| |
| - | *pSB1A3-1+N-EYFP#2
| |
| - | *GFP-dT
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| - |
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - | <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
| |
| - | <style type="text/css">
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| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
| |
| - | .qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
| |
| - |
| |
| - |
| |
| - | /* QuickMenu 0 */
| |
| - |
| |
| - | /*"""""""" (MAIN) Container""""""""*/
| |
| - | #qm0
| |
| - | {
| |
| - | width:auto;
| |
| - | background-color:transparent;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Items""""""""*/
| |
| - | #qm0 a
| |
| - | {
| |
| - | padding:5px 60px 3px 0px;
| |
| - | margin:0px 5px 0px 0px;
| |
| - | color:#FFD600;
| |
| - | font-family:Arial;
| |
| - | font-size:12px;
| |
| - | text-decoration:none;
| |
| - | font-weight:bold;
| |
| - | border-width:0px 0px 8px 0px;
| |
| - | border-style:solid;
| |
| - | border-color:#FFD600;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State""""""""*/
| |
| - | #qm0 a:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 li:hover>a
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Parent Items""""""""*/
| |
| - | #qm0 .qmparent
| |
| - | {
| |
| - | background-repeat:no-repeat;
| |
| - | background-position:95% 50%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Active State""""""""*/
| |
| - | body #qm0 .qmactive, body #qm0 .qmactive:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | text-decoration:none;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Container""""""""*/
| |
| - | #qm0 div, #qm0 ul
| |
| - | {
| |
| - | padding:10px 5px 10px 5px;
| |
| - | background-color:#EEEEEE;
| |
| - | border-width:1px;
| |
| - | border-style:solid;
| |
| - | border-color:#3C13AF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Items""""""""*/
| |
| - | #qm0 div a, #qm0 ul a
| |
| - | {
| |
| - | padding:3px 5px 3px 5px;
| |
| - | background-color:transparent;
| |
| - | color:#230672;
| |
| - | font-size:11px;
| |
| - | font-weight:normal;
| |
| - | border-width:0px;
| |
| - | border-style:none;
| |
| - | border-color:#230672;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State""""""""*/
| |
| - | #qm0 div a:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 ul li:hover>a
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Parent Items""""""""*/
| |
| - | #qm0 div .qmparent, #qm0 ul .qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_0.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Active State""""""""*/
| |
| - | body #qm0 div .qmactive, body #qm0 div .qmactive:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | color:#FFFFFF;
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| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Titles""""""""*/
| |
| - | #qm0 .qmtitle
| |
| - | {
| |
| - | margin:2px 5px 5px 5px;
| |
| - | color:#222222;
| |
| - | font-family:Arial;
| |
| - | font-size:11px;
| |
| - | font-weight:bold;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Horizontal Dividers""""""""*/
| |
| - | #qm0 .qmdividerx
| |
| - | {
| |
| - | border-top-width:1px;
| |
| - | margin:4px 5px 4px 5px;
| |
| - | border-color:#999999;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 ul
| |
| - | {
| |
| - | width:100%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 li:hover > a.qmparent
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| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
| |
| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
| |
| - | <li class="qmclear"> </li></ul>
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| |
| - | </body>
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| |
| - |
| |
| - | ===September 14===
| |
| - |
| |
| - | '''Ashley'''
| |
| - |
| |
| - | PCR of EYFP, CFP and ECFP
| |
| - |
| |
| - | See Aug 6 for volumes
| |
| - |
| |
| - | CFP(1)=prefix primer and antisense C-term suffix
| |
| - |
| |
| - | CFP(2)=N-term prefix and antisense suffix
| |
| - |
| |
| - | EYFP(1)=prefix primer and antisense C-term suffix
| |
| - |
| |
| - | EYFP(2)=N-term prefix and antisense suffix
| |
| - |
| |
| - | ECFP(1)=prefix primer and antisense C-term suffix
| |
| - |
| |
| - | ECFP(2)=N-term prefix and antisense suffix
| |
| - |
| |
| - | Cycle: tail67
| |
| - |
| |
| - | ===Sept 16===
| |
| - |
| |
| - | '''Ashley'''
| |
| - |
| |
| - | Miniprep of mmS6-dT 1,2 and 3. Used Qiagen benchtop protocol and kit. Eluted with 50 µL buffer EB. Put into -20°C freezer orange tray.
| |
| - |
| |
| - | PCR Purification of restriction digest of PCR products.
| |
| - |
| |
| - |
| |
| - |
| |
| - | ===September 24===
| |
| - | '''Ashley, Kirsten'''
| |
| - |
| |
| - | 1% gel electrophoresis
| |
| - | 6uL ladder (1kb)
| |
| - | 5uL sample +1 uL 6x loading dye
| |
| - | 100V, 40min
| |
| - |
| |
| - | Lane
| |
| - | #1kb Ladder
| |
| - | #C-CFP-1
| |
| - | #C-ECFP-1
| |
| - | #N-EYFP-2
| |
| - | #N-CFP-2
| |
| - | #N-ECFP-2
| |
| - | #C-EYFP-1
| |
| - | #CFP-1 Purified PCR
| |
| - | #ECFP-1 Purified PCR
| |
| - | #EYFP-2 Purified PCR
| |
| - | #CFP-2 Purified PCR
| |
| - | #ECFP-1 Purified PCR
| |
| - | #EYFP-1 Purified PCR
| |
| - | #C-CFP(1)
| |
| - | #ECFP(1)
| |
| - | #C-EYFP(1)
| |
| - | #N-CFP(2)
| |
| - | #N-EYCFP(2)
| |
| - | #N-EYFP(2)
| |
| - | #1KB LADDER
| |
| - |
| |
| - | [[Image:Sept_24_-_control_for_PCR.png|250px]]
| |
| - |
| |
| - | Gel was run to check for DNAses, found that DPN-1 had DNAses.
| |
| - |
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
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| |
| - | <style type="text/css">
| |
| - |
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| - |
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| |
| - |
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| - | <script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' 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| - |
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| - | <script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
| |
| - |
| |
| - | <!-- Add-On Code: IE Over Select Fix -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
| |
| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
| |
| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
| |
| - | <li class="qmclear"> </li></ul>
| |
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| |
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| |
| - | <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
| |
| - | </body>
| |
| - | </html>
| |
| - |
| |
| - | ===Sept 25===
| |
| - | 1% agarose gel 100V, 40min
| |
| - |
| |
| - | Lane:
| |
| - | #1kb ladder
| |
| - | #mms6(EcoRI,SpeI)
| |
| - | #pLacI (SpeI, PstI)
| |
| - | #Lumazine-dT
| |
| - |
| |
| - | Only pLacI success, gell extracted and eluted with 50uL buffer EB.
| |
| - |
| |
| - | Miniprep of:
| |
| - | *3x mmS6-dT
| |
| - | *3x pSB1A3-mmS6
| |
| - | *2x cheZ-dT
| |
| - | *1x riboswitch-cheZ-dT
| |
| - | *2x GFP-dT
| |
| - | *3x C-EYFP-dT
| |
| - |
| |
| - | Restricted all of the above with EcoRI
| |
| - | *8uL DNA
| |
| - | *1uL 10x buffer tango
| |
| - | *1uL EcoRI
| |
| - |
| |
| - | for 1 hour.
| |
| - |
| |
| - | Restriction of fusion proteins with either EcoRI,SpeI or XbaI,PstI
| |
| - | *Heat inactivated a 60C for 10min
| |
| - |
| |
| - | Ran on a gel (1% agarose)100V,40min
| |
| - |
| |
| - | Lane:
| |
| - | #1kb ladder
| |
| - | #pSB-mmS6 #1
| |
| - | #pSB-mmS6 #2
| |
| - | #pSB-mmS6 #3
| |
| - | #mms6-dT #1
| |
| - | #mms6-dT #2
| |
| - | #mms6-dT #3
| |
| - | #EYFP C-term-dT #1
| |
| - | #EYFP C-term-dT #2
| |
| - | #EYFP C-term-dT #3
| |
| - | #GFP-dT#1
| |
| - | #GFP-dT#2
| |
| - | #Riboswitch-cheZ-dT
| |
| - | #cheZ-dT#1
| |
| - | #cheZ-dT#2
| |
| - | #C-CFP (EcoRI,SpeI)
| |
| - | #C-CFP (XbaI,PstI)
| |
| - | #C-ECFP (EcoRI,SpeI)
| |
| - | #C-ECFP (XbaI,PstI)
| |
| - | #1kb laddder
| |
| - |
| |
| - | [[Image:Sept_25-first_lanes.png|250px|left]]
| |
| - |
| |
| - | [[Image:Sept_25_far_lanes.png|250px|left]]
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | *Restriction of Fusion proteins with Dpn1
| |
| - | *Heat inactivated a 60C for 10min
| |
| - |
| |
| - | Lane:
| |
| - | #1kB ladder
| |
| - | #N-ECFP
| |
| - | #N-ECFP
| |
| - | #N-EYFP
| |
| - | #E-EYFP
| |
| - | #N-CFP
| |
| - | #N-CFP
| |
| - | #C-EYFP
| |
| - | #C-EYFP
| |
| - | #1kB ladder
| |
| - |
| |
| - |
| |
| - | Restriction of Lumazine-dT
| |
| - | *30uL DNA
| |
| - | *4uL 10x buffer
| |
| - | *3uL EcoRI
| |
| - | *3uL SpeI
| |
| - | Setup Liquid culture of the Mr. Gene mmS6 x3
| |
| - |
| |
| - | ===September 28===
| |
| - | '''Ashley'''
| |
| - |
| |
| - | 1% agarose gel
| |
| - | *5uL DNA+1uL 6x loading dye
| |
| - | *6uL ladder (1kb)
| |
| - |
| |
| - | Lane:
| |
| - | #1kb ladder
| |
| - | #N-ECFP (XbaI,PstI)
| |
| - | #N-ECFP (EcoRI, SpeI)
| |
| - | #N-EYFP(XbaI,PstI)
| |
| - | #N-EYFP(EcoRI, SpeI)
| |
| - | #N-CFP(XbaI,PstI)
| |
| - | #N-CFP(EcoRI, SpeI)
| |
| - | #C-EYFP(XbaI,PstI)
| |
| - | #C-EYFP(EcoRI, SpeI)
| |
| - | #1kb ladder
| |
| - |
| |
| - | Ran at 100V for 40 min
| |
| - |
| |
| - | [[Image:September_28th.jpg|250px]]
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - | <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
| |
| - | <style type="text/css">
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
| |
| - | .qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
| |
| - |
| |
| - |
| |
| - | /* QuickMenu 0 */
| |
| - |
| |
| - | /*"""""""" (MAIN) Container""""""""*/
| |
| - | #qm0
| |
| - | {
| |
| - | width:auto;
| |
| - | background-color:transparent;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Items""""""""*/
| |
| - | #qm0 a
| |
| - | {
| |
| - | padding:5px 60px 3px 0px;
| |
| - | margin:0px 5px 0px 0px;
| |
| - | color:#FFD600;
| |
| - | font-family:Arial;
| |
| - | font-size:12px;
| |
| - | text-decoration:none;
| |
| - | font-weight:bold;
| |
| - | border-width:0px 0px 8px 0px;
| |
| - | border-style:solid;
| |
| - | border-color:#FFD600;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State""""""""*/
| |
| - | #qm0 a:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 li:hover>a
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Parent Items""""""""*/
| |
| - | #qm0 .qmparent
| |
| - | {
| |
| - | background-repeat:no-repeat;
| |
| - | background-position:95% 50%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Active State""""""""*/
| |
| - | body #qm0 .qmactive, body #qm0 .qmactive:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | text-decoration:none;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Container""""""""*/
| |
| - | #qm0 div, #qm0 ul
| |
| - | {
| |
| - | padding:10px 5px 10px 5px;
| |
| - | background-color:#EEEEEE;
| |
| - | border-width:1px;
| |
| - | border-style:solid;
| |
| - | border-color:#3C13AF;
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| |
| - |
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| |
| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
| |
| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
| |
| - | <li class="qmclear"> </li></ul>
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| |
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| - | </html>
| |
| | | | |
| | == October == | | == October == |
Fri Oct 9 Overexpression pLac + sRBS-Lumazine-dT
Gel Extraction pLac-sRBS-Lumazine-dT
pBad-TetR-C-term FPs
pBad-TetR-N-term FPs
pTetR-N-term ECFP
C-term CFP (has pTetR)
Motility Media
"
