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| Line 285: |
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| | <li><a href="https://2009.igem.org/Team:Lethbridge/Criteria" style="width:1px;">Judging</a></li> | | <li><a href="https://2009.igem.org/Team:Lethbridge/Criteria" style="width:1px;">Judging</a></li> |
| | <li><a href="https://2009.igem.org/Team:Lethbridge/videos" style="width:125px;">Extra Information on SynBio</a></li> | | <li><a href="https://2009.igem.org/Team:Lethbridge/videos" style="width:125px;">Extra Information on SynBio</a></li> |
| - | <li class="qmclear"> </li></ul>
| |
| - |
| |
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| |
| - | <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
| |
| - | </body>
| |
| - | </html>
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | == October ==
| |
| - |
| |
| - | ===October 1===
| |
| - |
| |
| - | '''Roxanne'''
| |
| - | Gel for gel extraction
| |
| - |
| |
| - | Restriction
| |
| - | Ribo-Chez-dT
| |
| - | *2x CheZ-dT
| |
| - | *2x GFP-dT
| |
| - | *3x C-EYFP-dT
| |
| - |
| |
| - | Ligation
| |
| - | *C-ECFP-dT
| |
| - | *C-CFP-dT
| |
| - | *C-EYFP-dT
| |
| - | *mRBS-N-ECFP
| |
| - | *mRBS-N-EYFP
| |
| - | *pSB-C-EFP
| |
| - | *pSB-C-EYFP
| |
| - | *pSB-C-CFP
| |
| - | *pSB-N-ECFP
| |
| - | *pSB-N-EYFP
| |
| - |
| |
| - | Aliquoted DH5α cells
| |
| - |
| |
| - | '''Ashley'''
| |
| - |
| |
| - | {|
| |
| - | |Tube
| |
| - | |'''Tube + Gel'''
| |
| - | |Gel
| |
| - | |-
| |
| - | |1.1226g
| |
| - | |'''1.4931g'''
| |
| - | |0.2671g
| |
| - | |-
| |
| - | |1.012g
| |
| - | |'''1.2302g'''
| |
| - | |0.1290g
| |
| - | |}
| |
| - |
| |
| - | Eluted with 50uL buffer EB
| |
| - |
| |
| - | Ligation
| |
| - |
| |
| - | {|
| |
| - | |
| |
| - | |'''1:3'''
| |
| - | |2:6
| |
| - | |-
| |
| - | |lum-dT
| |
| - | |'''3uL'''
| |
| - | |6uL
| |
| - | |-
| |
| - | |SRBS
| |
| - | |'''1uL'''
| |
| - | |2uL
| |
| - | |-
| |
| - | |Ligase Buffer
| |
| - | |'''1uL'''
| |
| - | |2uL
| |
| - | |-
| |
| - | |Water
| |
| - | |4.5uL
| |
| - | |9uL
| |
| - | |-
| |
| - | |Ligase
| |
| - | |0.5uL
| |
| - | |1uL
| |
| - | |}
| |
| - | ===Oct 2===
| |
| - | Pick Colonies:
| |
| - | *sRBS + Lumazine-dT
| |
| - | *mRBS + N-term FPs
| |
| - | *C-term FPs + dT
| |
| - | *pSB + N-term FPs
| |
| - | *pSB + N-term FPs
| |
| - |
| |
| - | Gel Extraction:
| |
| - | *Riboswitch-cheZ-dT
| |
| - | *2x cheZ-dT (in case Ribo-cheZ-dT isn't actually there)
| |
| - | *3x C-EYFP-dT
| |
| - | *2x GFP-dT
| |
| - |
| |
| - | Liquid Culture:
| |
| - | *Riboswitch-cheZ-dT
| |
| - | *2x cheZ-dT (in case Ribo-cheZ-dT isn't actually there)
| |
| - | *3x C-EYFP-dT
| |
| - | *2x GFP-dT
| |
| - |
| |
| - | Miniprep:
| |
| - | *Mr. Gene mms6
| |
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| - | <script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
| |
| - |
| |
| - | <!-- Add-On Code: IE Over Select Fix -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
| |
| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
| |
| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
| |
| - | <li class="qmclear"> </li></ul>
| |
| - |
| |
| - | <!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
| |
| - | <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
| |
| - | </body>
| |
| - | </html>
| |
| - |
| |
| - | ===Sat Oct 3===
| |
| - | Miniprep:
| |
| - | *sRBS + Lumazine-dT
| |
| - | *mRBS + N-term FPs
| |
| - | *C-term FPs + dT
| |
| - | *pSB + N-term FPs
| |
| - | *pSB + N-term FPs
| |
| - | *sRBS + Lumazine-dT
| |
| - | Restriction:
| |
| - |
| |
| - | Analytic and Prep:
| |
| - | *mRBS + N-term FPs
| |
| - | *C-term FPs + dT
| |
| - | *pSB + N-term FPs
| |
| - | *pSB + N-term FPs
| |
| - |
| |
| - | Analytic only:
| |
| - | *Mr. Gene mms6
| |
| - |
| |
| - | Prep only:
| |
| - | *pBAD-TetR
| |
| - |
| |
| - | ===Oct 5===
| |
| - |
| |
| - | Gel extraction:
| |
| - | *sRBS + Lumazine-dT
| |
| - | *mRBS + N-term FPs
| |
| - | *C-term FPs + dT
| |
| - | *pSB + N-term FPs
| |
| - | *pSB + N-term FPs
| |
| - |
| |
| - | Ligation:
| |
| - | *pStrong + Riboswitch-cheZ-dT
| |
| - | *Riboswitch-GFP-dT
| |
| - | *pLac + sRBS-Lumazine-dT
| |
| - | *pBad-TetR + C-term FPs
| |
| - | *pBad-TetR + N-term FPs
| |
| - | *pTetR + N-term
| |
| - |
| |
| - | Transformation:
| |
| - | *pLac + sRBS-Lumazine-dT
| |
| - | *pBad-TetR + C-term FPs
| |
| - | *pBad-TetR + N-term FPs
| |
| - | *pTetR + N-term ECFP
| |
| - | *pStrong + Riboswitch-cheZ-dT
| |
| - | *Riboswitch-GFP-dT
| |
| - |
| |
| - | Sequencing:
| |
| - | *sRBS + Lumazine-dT
| |
| - | *mRBS + N-term FPs
| |
| - | *C-term FPs + dT
| |
| - | *pSB + N-term FPs
| |
| - | *pSB + N-term FPs
| |
| - | *Mr. Gene mms6
| |
| - |
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - | <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
| |
| - | <style type="text/css">
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
| |
| - | .qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
| |
| - |
| |
| - |
| |
| - | /* QuickMenu 0 */
| |
| - |
| |
| - | /*"""""""" (MAIN) Container""""""""*/
| |
| - | #qm0
| |
| - | {
| |
| - | width:auto;
| |
| - | background-color:transparent;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Items""""""""*/
| |
| - | #qm0 a
| |
| - | {
| |
| - | padding:5px 60px 3px 0px;
| |
| - | margin:0px 5px 0px 0px;
| |
| - | color:#FFD600;
| |
| - | font-family:Arial;
| |
| - | font-size:12px;
| |
| - | text-decoration:none;
| |
| - | font-weight:bold;
| |
| - | border-width:0px 0px 8px 0px;
| |
| - | border-style:solid;
| |
| - | border-color:#FFD600;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State""""""""*/
| |
| - | #qm0 a:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 li:hover>a
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Parent Items""""""""*/
| |
| - | #qm0 .qmparent
| |
| - | {
| |
| - | background-repeat:no-repeat;
| |
| - | background-position:95% 50%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Active State""""""""*/
| |
| - | body #qm0 .qmactive, body #qm0 .qmactive:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | text-decoration:none;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Container""""""""*/
| |
| - | #qm0 div, #qm0 ul
| |
| - | {
| |
| - | padding:10px 5px 10px 5px;
| |
| - | background-color:#EEEEEE;
| |
| - | border-width:1px;
| |
| - | border-style:solid;
| |
| - | border-color:#3C13AF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Items""""""""*/
| |
| - | #qm0 div a, #qm0 ul a
| |
| - | {
| |
| - | padding:3px 5px 3px 5px;
| |
| - | background-color:transparent;
| |
| - | color:#230672;
| |
| - | font-size:11px;
| |
| - | font-weight:normal;
| |
| - | border-width:0px;
| |
| - | border-style:none;
| |
| - | border-color:#230672;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State""""""""*/
| |
| - | #qm0 div a:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 ul li:hover>a
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Parent Items""""""""*/
| |
| - | #qm0 div .qmparent, #qm0 ul .qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_0.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Active State""""""""*/
| |
| - | body #qm0 div .qmactive, body #qm0 div .qmactive:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | color:#FFFFFF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Titles""""""""*/
| |
| - | #qm0 .qmtitle
| |
| - | {
| |
| - | margin:2px 5px 5px 5px;
| |
| - | color:#222222;
| |
| - | font-family:Arial;
| |
| - | font-size:11px;
| |
| - | font-weight:bold;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Horizontal Dividers""""""""*/
| |
| - | #qm0 .qmdividerx
| |
| - | {
| |
| - | border-top-width:1px;
| |
| - | margin:4px 5px 4px 5px;
| |
| - | border-color:#999999;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 ul
| |
| - | {
| |
| - | width:100%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 li:hover > a.qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | </style>
| |
| - |
| |
| - | <!-- Add-On Core Code (Remove when not using any add-on's) -->
| |
| - | <style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
| |
| - |
| |
| - | <!-- Add-On Settings -->
| |
| - | <script type="text/JavaScript">
| |
| - |
| |
| - | /******* Menu 0 Add-On Settings *******/
| |
| - | var a = qmad.qm0 = new Object();
| |
| - |
| |
| - | // Match Widths Add On
| |
| - | a.mwidths_active = true;
| |
| - |
| |
| - | // IE Over Select Fix Add On
| |
| - | a.overselects_active = true;
| |
| - |
| |
| - | </script>
| |
| - |
| |
| - | <!-- Core QuickMenu Code -->
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| - | <script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' 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| - |
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| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
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| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
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| - | <li class="qmclear"> </li></ul>
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| - | <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
| |
| - | </body>
| |
| - | </html>
| |
| - |
| |
| - | ===Tues Oct 6===
| |
| - | Pick Colonies:
| |
| - | *pLac + sRBS-Lumazine-dT
| |
| - | *pBad-TetR + C-term FPs
| |
| - | *pBad-TetR + N-term FPs
| |
| - | *pTetR + N-term ECFP
| |
| - | *pStrong + Riboswitch-cheZ-dT
| |
| - | *Riboswitch-GFP-dT
| |
| - |
| |
| - | ===Wed Oct 7===
| |
| - |
| |
| - | Miniprep:
| |
| - | *pLac + sRBS-Lumazine-dT
| |
| - | *pBad-TetR + C-term FPs
| |
| - | *pBad-TetR + N-term FPs
| |
| - | *pTetR + C-term ECFP
| |
| - | *pTetR + N-term ECFP
| |
| - | *pStrong + Riboswitch-cheZ-dT
| |
| - | *Riboswitch-GFP-dT
| |
| - |
| |
| - | Analytic Restriction:
| |
| - | *pLac + sRBS-Lumazine-dT
| |
| - | *pBad-TetR + C-term FPs
| |
| - | *pBad-TetR + N-term FPs
| |
| - | *pTetR + N-term ECFP
| |
| - | *pStrong + Riboswitch-cheZ-dT
| |
| - | *Riboswitch-GFP-dT
| |
| - |
| |
| - | Prep Restriction:
| |
| - | *Riboswitch-GFP-dT
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - | <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
| |
| - | <style type="text/css">
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| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
| |
| - | .qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
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| - |
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| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
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| - |
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| - |
| |
| - | /* QuickMenu 0 */
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| - |
| |
| - | /*"""""""" (MAIN) Container""""""""*/
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| - | #qm0
| |
| - | {
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| - | width:auto;
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| - | background-color:transparent;
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| - | color:#FFD600;
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| - | font-family:Arial;
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| - | font-size:12px;
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| - | text-decoration:none;
| |
| - | font-weight:bold;
| |
| - | border-width:0px 0px 8px 0px;
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| - | border-style:solid;
| |
| - | border-color:#FFD600;
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| - | /*"""""""" (MAIN) Hover State""""""""*/
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| - | #qm0 a:hover
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| - | border-color:#FFAD00;
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| - | }
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| - |
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| - |
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| - | /*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 li:hover>a
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| - | {
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| - | color:#3C13AF;
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| - | border-color:#FFAD00;
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| - | }
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| - |
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| - |
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| - | /*"""""""" (MAIN) Parent Items""""""""*/
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| - | #qm0 .qmparent
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| - | background-repeat:no-repeat;
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| - | background-position:95% 50%;
| |
| - | }
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| - |
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| - |
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| - | /*"""""""" (MAIN) Active State""""""""*/
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| - | body #qm0 .qmactive, body #qm0 .qmactive:hover
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| - | {
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| - | color:#3C13AF;
| |
| - | text-decoration:none;
| |
| - | border-color:#FFAD00;
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| - | }
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| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Container""""""""*/
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| - | #qm0 div, #qm0 ul
| |
| - | {
| |
| - | padding:10px 5px 10px 5px;
| |
| - | background-color:#EEEEEE;
| |
| - | border-width:1px;
| |
| - | border-style:solid;
| |
| - | border-color:#3C13AF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Items""""""""*/
| |
| - | #qm0 div a, #qm0 ul a
| |
| - | {
| |
| - | padding:3px 5px 3px 5px;
| |
| - | background-color:transparent;
| |
| - | color:#230672;
| |
| - | font-size:11px;
| |
| - | font-weight:normal;
| |
| - | border-width:0px;
| |
| - | border-style:none;
| |
| - | border-color:#230672;
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| - | }
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| - |
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| - |
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| - | /*"""""""" (SUB) Hover State""""""""*/
| |
| - | #qm0 div a:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
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| - | }
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| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 ul li:hover>a
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
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| - | text-decoration:none;
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| - | }
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| - |
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| - |
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| - | /*"""""""" (SUB) Parent Items""""""""*/
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| - | #qm0 div .qmparent, #qm0 ul .qmparent
| |
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| - | background-image:url(qmimages/arrow_0.gif);
| |
| - | }
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| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Active State""""""""*/
| |
| - | body #qm0 div .qmactive, body #qm0 div .qmactive:hover
| |
| - | {
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| - | background-color:#476DD5;
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | color:#FFFFFF;
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| - | }
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| - |
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| - |
| |
| - | /*"""""""" Individual Titles""""""""*/
| |
| - | #qm0 .qmtitle
| |
| - | {
| |
| - | margin:2px 5px 5px 5px;
| |
| - | color:#222222;
| |
| - | font-family:Arial;
| |
| - | font-size:11px;
| |
| - | font-weight:bold;
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| - | }
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| - |
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| - |
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| - | /*"""""""" Individual Horizontal Dividers""""""""*/
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| - | #qm0 .qmdividerx
| |
| - | {
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| - | border-top-width:1px;
| |
| - | margin:4px 5px 4px 5px;
| |
| - | border-color:#999999;
| |
| - | }
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| - |
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| - |
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| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 ul
| |
| - | {
| |
| - | width:100%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 li:hover > a.qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | </style>
| |
| - |
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| - | <!-- Add-On Core Code (Remove when not using any add-on's) -->
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| - | <style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
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| - |
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| - | <!-- Add-On Settings -->
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| - | <script type="text/JavaScript">
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| - |
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| - | /******* Menu 0 Add-On Settings *******/
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| - | var a = qmad.qm0 = new Object();
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| - |
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| - | // Match Widths Add On
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| - | a.mwidths_active = true;
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| - |
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| - | // IE Over Select Fix Add On
| |
| - | a.overselects_active = true;
| |
| - |
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| - | </script>
| |
| - |
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| - | <!-- Core QuickMenu Code -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' 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| |
| - |
| |
| - | <!-- Add-On Code: Match Widths -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
| |
| - |
| |
| - | <!-- Add-On Code: IE Over Select Fix -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
| |
| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
| |
| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
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| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
| |
| - | <li class="qmclear"> </li></ul>
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| - |
| |
| - | <!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
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| - | <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
| |
| - | </body>
| |
| - | </html>
| |
| - |
| |
| - | ===Thurs Oct 8===
| |
| - | Gel Extraction:
| |
| - | *Riboswitch-GFP-dT
| |
| - |
| |
| - | Ligation:
| |
| - | *pStrong + Riboswitch-GFP-dT
| |
| - |
| |
| - | Transformation:
| |
| - | *pStrong + Riboswitch-GFP-dT
| |
| - |
| |
| - | Sequencing:
| |
| - | *pLac-sRBS-Lumazine-dT
| |
| - | *pBad-TetR-C-term FPs
| |
| - | *pBad-TetR-N-term FPs
| |
| - | *pTetR-N-term ECFP
| |
| - | *pStrong-Riboswitch-cheZ-dT
| |
| - | *Riboswitch-GFP-dT
| |
| - |
| |
| - | Maxiprep:
| |
| - | *pLac-sRBS-Lumazine-dT
| |
| - | *pBad-TetR-C-term FPs
| |
| - | *pBad-TetR-N-term FPs
| |
| - | *pTetR-N-term ECFP
| |
| - | *pStrong-Riboswitch-GFP-dT
| |
| - |
| |
| - | Restriction:
| |
| - | *pLac-sRBS-Lumazine-
| |
| - |
| |
| - | Analytic and Prep:
| |
| - | *pBad-TetR-C-term FPs
| |
| - | *pBad-TetR-N-term FPs
| |
| - | *pTetR-N-term ECFP
| |
| - | *C-term CFP (has pTetR)
| |
| - |
| |
| - | Analytic Only:
| |
| - | *pStrong-Riboswitch-GFP-dT
| |
| - |
| |
| - | Fri Oct 9 Overexpression pLac + sRBS-Lumazine-dT
| |
| - | Gel Extraction pLac-sRBS-Lumazine-dT
| |
| - | pBad-TetR-C-term FPs
| |
| - | pBad-TetR-N-term FPs
| |
| - | pTetR-N-term ECFP
| |
| - | C-term CFP (has pTetR)
| |
| - | Motility Media
| |
| - | ===Oct 12===
| |
| - | Ligation:
| |
| - | *pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
| |
| - | *pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP
| |
| - |
| |
| - | Transformation:
| |
| - | *pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
| |
| - | *pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP
| |
| - |
| |
| - | Plate Testing:
| |
| - | *pStrong-Riboswitch-GFP-dT
| |
| - |
| |
| - | Motility Test:
| |
| - | *pStrong + Riboswitch-cheZ-dT
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - | <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
| |
| - | <style type="text/css">
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| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
| |
| - | .qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
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| - |
| |
| - |
| |
| - | /* QuickMenu 0 */
| |
| - |
| |
| - | /*"""""""" (MAIN) Container""""""""*/
| |
| - | #qm0
| |
| - | {
| |
| - | width:auto;
| |
| - | background-color:transparent;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Items""""""""*/
| |
| - | #qm0 a
| |
| - | {
| |
| - | padding:5px 60px 3px 0px;
| |
| - | margin:0px 5px 0px 0px;
| |
| - | color:#FFD600;
| |
| - | font-family:Arial;
| |
| - | font-size:12px;
| |
| - | text-decoration:none;
| |
| - | font-weight:bold;
| |
| - | border-width:0px 0px 8px 0px;
| |
| - | border-style:solid;
| |
| - | border-color:#FFD600;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State""""""""*/
| |
| - | #qm0 a:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 li:hover>a
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Parent Items""""""""*/
| |
| - | #qm0 .qmparent
| |
| - | {
| |
| - | background-repeat:no-repeat;
| |
| - | background-position:95% 50%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Active State""""""""*/
| |
| - | body #qm0 .qmactive, body #qm0 .qmactive:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | text-decoration:none;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Container""""""""*/
| |
| - | #qm0 div, #qm0 ul
| |
| - | {
| |
| - | padding:10px 5px 10px 5px;
| |
| - | background-color:#EEEEEE;
| |
| - | border-width:1px;
| |
| - | border-style:solid;
| |
| - | border-color:#3C13AF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Items""""""""*/
| |
| - | #qm0 div a, #qm0 ul a
| |
| - | {
| |
| - | padding:3px 5px 3px 5px;
| |
| - | background-color:transparent;
| |
| - | color:#230672;
| |
| - | font-size:11px;
| |
| - | font-weight:normal;
| |
| - | border-width:0px;
| |
| - | border-style:none;
| |
| - | border-color:#230672;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State""""""""*/
| |
| - | #qm0 div a:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 ul li:hover>a
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Parent Items""""""""*/
| |
| - | #qm0 div .qmparent, #qm0 ul .qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_0.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Active State""""""""*/
| |
| - | body #qm0 div .qmactive, body #qm0 div .qmactive:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | color:#FFFFFF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Titles""""""""*/
| |
| - | #qm0 .qmtitle
| |
| - | {
| |
| - | margin:2px 5px 5px 5px;
| |
| - | color:#222222;
| |
| - | font-family:Arial;
| |
| - | font-size:11px;
| |
| - | font-weight:bold;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Horizontal Dividers""""""""*/
| |
| - | #qm0 .qmdividerx
| |
| - | {
| |
| - | border-top-width:1px;
| |
| - | margin:4px 5px 4px 5px;
| |
| - | border-color:#999999;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 ul
| |
| - | {
| |
| - | width:100%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 li:hover > a.qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | </style>
| |
| - |
| |
| - | <!-- Add-On Core Code (Remove when not using any add-on's) -->
| |
| - | <style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
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| - |
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| - | <!-- Add-On Settings -->
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| - | <script type="text/JavaScript">
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| - |
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| - | /******* Menu 0 Add-On Settings *******/
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| - | var a = qmad.qm0 = new Object();
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| - |
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| - | // Match Widths Add On
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| - | a.mwidths_active = true;
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| - | // IE Over Select Fix Add On
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| - | </script>
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| - |
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| - | <!-- Core QuickMenu Code -->
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| - | <script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' 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| - |
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| - | <!-- Add-On Code: Match Widths -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
| |
| - |
| |
| - | <!-- Add-On Code: IE Over Select Fix -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
| |
| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
| |
| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
| |
| - | <li class="qmclear"> </li></ul>
| |
| - |
| |
| - | <!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
| |
| - | <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
| |
| - | </body>
| |
| - | </html>
| |
| - |
| |
| - | ===Oct 13===
| |
| - | Pick Colonies
| |
| - | *pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
| |
| - | *pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP
| |
| - |
| |
| - | Analysis of Plate and Motility Tests
| |
| - |
| |
| - | ===Wed Oct 14===
| |
| - | Miniprep:
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP
| |
| - |
| |
| - | Restriction:
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP
| |
| - |
| |
| - | ===Thurs Oct 15===
| |
| - | Gel Extraction:
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP
| |
| - |
| |
| - | Ligation:
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP
| |
| - |
| |
| - | Transformation:
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP
| |
| - |
| |
| - | Expression Testing:
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - | <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
| |
| - | <style type="text/css">
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
| |
| - | .qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
| |
| - |
| |
| - |
| |
| - | /* QuickMenu 0 */
| |
| - |
| |
| - | /*"""""""" (MAIN) Container""""""""*/
| |
| - | #qm0
| |
| - | {
| |
| - | width:auto;
| |
| - | background-color:transparent;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Items""""""""*/
| |
| - | #qm0 a
| |
| - | {
| |
| - | padding:5px 60px 3px 0px;
| |
| - | margin:0px 5px 0px 0px;
| |
| - | color:#FFD600;
| |
| - | font-family:Arial;
| |
| - | font-size:12px;
| |
| - | text-decoration:none;
| |
| - | font-weight:bold;
| |
| - | border-width:0px 0px 8px 0px;
| |
| - | border-style:solid;
| |
| - | border-color:#FFD600;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State""""""""*/
| |
| - | #qm0 a:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 li:hover>a
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Parent Items""""""""*/
| |
| - | #qm0 .qmparent
| |
| - | {
| |
| - | background-repeat:no-repeat;
| |
| - | background-position:95% 50%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Active State""""""""*/
| |
| - | body #qm0 .qmactive, body #qm0 .qmactive:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | text-decoration:none;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Container""""""""*/
| |
| - | #qm0 div, #qm0 ul
| |
| - | {
| |
| - | padding:10px 5px 10px 5px;
| |
| - | background-color:#EEEEEE;
| |
| - | border-width:1px;
| |
| - | border-style:solid;
| |
| - | border-color:#3C13AF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Items""""""""*/
| |
| - | #qm0 div a, #qm0 ul a
| |
| - | {
| |
| - | padding:3px 5px 3px 5px;
| |
| - | background-color:transparent;
| |
| - | color:#230672;
| |
| - | font-size:11px;
| |
| - | font-weight:normal;
| |
| - | border-width:0px;
| |
| - | border-style:none;
| |
| - | border-color:#230672;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State""""""""*/
| |
| - | #qm0 div a:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 ul li:hover>a
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Parent Items""""""""*/
| |
| - | #qm0 div .qmparent, #qm0 ul .qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_0.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Active State""""""""*/
| |
| - | body #qm0 div .qmactive, body #qm0 div .qmactive:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | color:#FFFFFF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Titles""""""""*/
| |
| - | #qm0 .qmtitle
| |
| - | {
| |
| - | margin:2px 5px 5px 5px;
| |
| - | color:#222222;
| |
| - | font-family:Arial;
| |
| - | font-size:11px;
| |
| - | font-weight:bold;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Horizontal Dividers""""""""*/
| |
| - | #qm0 .qmdividerx
| |
| - | {
| |
| - | border-top-width:1px;
| |
| - | margin:4px 5px 4px 5px;
| |
| - | border-color:#999999;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 ul
| |
| - | {
| |
| - | width:100%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 li:hover > a.qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | </style>
| |
| - |
| |
| - | <!-- Add-On Core Code (Remove when not using any add-on's) -->
| |
| - | <style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
| |
| - |
| |
| - | <!-- Add-On Settings -->
| |
| - | <script type="text/JavaScript">
| |
| - |
| |
| - | /******* Menu 0 Add-On Settings *******/
| |
| - | var a = qmad.qm0 = new Object();
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| - |
| |
| - | // Match Widths Add On
| |
| - | a.mwidths_active = true;
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| - |
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| - | // IE Over Select Fix Add On
| |
| - | a.overselects_active = true;
| |
| - |
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| - | </script>
| |
| - |
| |
| - | <!-- Core QuickMenu Code -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' 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| |
| - |
| |
| - | <!-- Add-On Code: Match Widths -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
| |
| - |
| |
| - | <!-- Add-On Code: IE Over Select Fix -->
| |
| - | <script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
| |
| - | </head>
| |
| - | <body>
| |
| - | <ul id="qm0" class="qmmc">
| |
| - |
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
| |
| - | <li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
| |
| - | <li class="qmclear"> </li></ul>
| |
| - |
| |
| - | <!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
| |
| - | <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
| |
| - | </body>
| |
| - | </html>
| |
| - |
| |
| - | ===Fri Oct 16===
| |
| - | Pick Colonies:
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP
| |
| - |
| |
| - | ===Sat Oct 17===
| |
| - | Maxiprep:
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
| |
| - | *pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP
| |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - | <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
| |
| - | <style type="text/css">
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
| |
| - | .qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
| |
| - |
| |
| - |
| |
| - | /*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
| |
| - |
| |
| - |
| |
| - | /* QuickMenu 0 */
| |
| - |
| |
| - | /*"""""""" (MAIN) Container""""""""*/
| |
| - | #qm0
| |
| - | {
| |
| - | width:auto;
| |
| - | background-color:transparent;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Items""""""""*/
| |
| - | #qm0 a
| |
| - | {
| |
| - | padding:5px 60px 3px 0px;
| |
| - | margin:0px 5px 0px 0px;
| |
| - | color:#FFD600;
| |
| - | font-family:Arial;
| |
| - | font-size:12px;
| |
| - | text-decoration:none;
| |
| - | font-weight:bold;
| |
| - | border-width:0px 0px 8px 0px;
| |
| - | border-style:solid;
| |
| - | border-color:#FFD600;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State""""""""*/
| |
| - | #qm0 a:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 li:hover>a
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Parent Items""""""""*/
| |
| - | #qm0 .qmparent
| |
| - | {
| |
| - | background-repeat:no-repeat;
| |
| - | background-position:95% 50%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (MAIN) Active State""""""""*/
| |
| - | body #qm0 .qmactive, body #qm0 .qmactive:hover
| |
| - | {
| |
| - | color:#3C13AF;
| |
| - | text-decoration:none;
| |
| - | border-color:#FFAD00;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Container""""""""*/
| |
| - | #qm0 div, #qm0 ul
| |
| - | {
| |
| - | padding:10px 5px 10px 5px;
| |
| - | background-color:#EEEEEE;
| |
| - | border-width:1px;
| |
| - | border-style:solid;
| |
| - | border-color:#3C13AF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Items""""""""*/
| |
| - | #qm0 div a, #qm0 ul a
| |
| - | {
| |
| - | padding:3px 5px 3px 5px;
| |
| - | background-color:transparent;
| |
| - | color:#230672;
| |
| - | font-size:11px;
| |
| - | font-weight:normal;
| |
| - | border-width:0px;
| |
| - | border-style:none;
| |
| - | border-color:#230672;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State""""""""*/
| |
| - | #qm0 div a:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
| |
| - | #qm0 ul li:hover>a
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Parent Items""""""""*/
| |
| - | #qm0 div .qmparent, #qm0 ul .qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_0.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" (SUB) Active State""""""""*/
| |
| - | body #qm0 div .qmactive, body #qm0 div .qmactive:hover
| |
| - | {
| |
| - | background-color:#476DD5;
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | color:#FFFFFF;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Titles""""""""*/
| |
| - | #qm0 .qmtitle
| |
| - | {
| |
| - | margin:2px 5px 5px 5px;
| |
| - | color:#222222;
| |
| - | font-family:Arial;
| |
| - | font-size:11px;
| |
| - | font-weight:bold;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Individual Horizontal Dividers""""""""*/
| |
| - | #qm0 .qmdividerx
| |
| - | {
| |
| - | border-top-width:1px;
| |
| - | margin:4px 5px 4px 5px;
| |
| - | border-color:#999999;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 ul
| |
| - | {
| |
| - | width:100%;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | /*"""""""" Custom Rule""""""""*/
| |
| - | ul#qm0 li:hover > a.qmparent
| |
| - | {
| |
| - | background-image:url(qmimages/arrow_1.gif);
| |
| - | }
| |
| - |
| |
| - |
| |
| - | </style>
| |
| - |
| |
| - | <!-- Add-On Core Code (Remove when not using any add-on's) -->
| |
| - | <style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
| |
| - |
| |
| - | <!-- Add-On Settings -->
| |
| - | <script type="text/JavaScript">
| |
| - |
| |
| - | /******* Menu 0 Add-On Settings *******/
| |
| - | var a = qmad.qm0 = new Object();
| |
| - |
| |
| - | // Match Widths Add On
| |
| - | a.mwidths_active = true;
| |
| - |
| |
| - | // IE Over Select Fix Add On
| |
| - | a.overselects_active = true;
| |
| - |
| |
| - | </script>
| |
| - |
| |
| - | <!-- Core QuickMenu Code -->
| |
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