Team:Lethbridge/Notebook

From 2009.igem.org

(Difference between revisions)
Line 278: Line 278:
<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Favourites">Favourites</a></li>
<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Favourites">Favourites</a></li>
<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Individual_Parts">Individual Parts</a></li>
<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Individual_Parts">Individual Parts</a></li>
-
<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Constructs.2FDevices">Constructs</a></li>
+
<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#onstructs.2FDevices">Constructs</a></li>
<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediates</a></li>
<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediates</a></li>
</ul></li>
</ul></li>
Line 285: Line 285:
<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria" style="width:1px;">Judging</a></li>
<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria" style="width:1px;">Judging</a></li>
<li><a href="https://2009.igem.org/Team:Lethbridge/videos" style="width:125px;">Extra Information on SynBio</a></li>
<li><a href="https://2009.igem.org/Team:Lethbridge/videos" style="width:125px;">Extra Information on SynBio</a></li>
 +
<li class="qmclear">&nbsp;</li></ul>
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 +
 +
== October ==
 +
 +
===October 1===
 +
 +
'''Roxanne'''
 +
Gel for gel extraction
 +
 +
Restriction
 +
Ribo-Chez-dT
 +
*2x CheZ-dT
 +
*2x GFP-dT
 +
*3x C-EYFP-dT
 +
 +
Ligation
 +
*C-ECFP-dT
 +
*C-CFP-dT
 +
*C-EYFP-dT
 +
*mRBS-N-ECFP
 +
*mRBS-N-EYFP
 +
*pSB-C-EFP
 +
*pSB-C-EYFP
 +
*pSB-C-CFP
 +
*pSB-N-ECFP
 +
*pSB-N-EYFP
 +
 +
Aliquoted DH5α cells
 +
 +
'''Ashley'''
 +
 +
{|
 +
|Tube
 +
|'''Tube + Gel'''
 +
|Gel
 +
|-
 +
|1.1226g
 +
|'''1.4931g'''
 +
|0.2671g
 +
|-
 +
|1.012g
 +
|'''1.2302g'''
 +
|0.1290g
 +
|}
 +
 +
Eluted with 50uL buffer EB
 +
 +
Ligation
 +
 +
{|
 +
|
 +
|'''1:3'''
 +
|2:6
 +
|-
 +
|lum-dT
 +
|'''3uL'''
 +
|6uL
 +
|-
 +
|SRBS
 +
|'''1uL'''
 +
|2uL
 +
|-
 +
|Ligase Buffer
 +
|'''1uL'''
 +
|2uL
 +
|-
 +
|Water
 +
|4.5uL
 +
|9uL
 +
|-
 +
|Ligase
 +
|0.5uL
 +
|1uL
 +
|}
 +
===Oct 2===
 +
Pick Colonies:
 +
*sRBS + Lumazine-dT
 +
*mRBS + N-term FPs
 +
*C-term FPs + dT
 +
*pSB + N-term FPs
 +
*pSB + N-term FPs
 +
 +
Gel Extraction:
 +
*Riboswitch-cheZ-dT
 +
*2x cheZ-dT (in case Ribo-cheZ-dT isn't actually there)
 +
*3x C-EYFP-dT
 +
*2x GFP-dT
 +
 +
Liquid Culture:
 +
*Riboswitch-cheZ-dT
 +
*2x cheZ-dT (in case Ribo-cheZ-dT isn't actually there)
 +
*3x C-EYFP-dT
 +
*2x GFP-dT
 +
 +
Miniprep:
 +
*Mr. Gene mms6
 +
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<li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
 +
<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
 +
<li class="qmclear">&nbsp;</li></ul>
 +
 +
<!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
 +
<script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
 +
</body>
 +
</html>
 +
 +
===Sat Oct 3===
 +
Miniprep:
 +
*sRBS + Lumazine-dT
 +
*mRBS + N-term FPs
 +
*C-term FPs + dT
 +
*pSB + N-term FPs
 +
*pSB + N-term FPs
 +
*sRBS + Lumazine-dT
 +
Restriction:
 +
 +
Analytic and Prep:
 +
*mRBS + N-term FPs
 +
*C-term FPs + dT
 +
*pSB + N-term FPs
 +
*pSB + N-term FPs
 +
 +
Analytic only:
 +
*Mr. Gene mms6
 +
 +
Prep only:
 +
*pBAD-TetR
 +
 +
===Oct 5===
 +
 +
Gel extraction:
 +
*sRBS + Lumazine-dT
 +
*mRBS + N-term FPs
 +
*C-term FPs + dT
 +
*pSB + N-term FPs
 +
*pSB + N-term FPs
 +
 +
Ligation:
 +
*pStrong + Riboswitch-cheZ-dT
 +
*Riboswitch-GFP-dT
 +
*pLac + sRBS-Lumazine-dT
 +
*pBad-TetR + C-term FPs
 +
*pBad-TetR + N-term FPs
 +
*pTetR + N-term
 +
 +
Transformation:
 +
*pLac + sRBS-Lumazine-dT
 +
*pBad-TetR + C-term FPs
 +
*pBad-TetR + N-term FPs
 +
*pTetR + N-term ECFP
 +
*pStrong + Riboswitch-cheZ-dT
 +
*Riboswitch-GFP-dT
 +
 +
Sequencing:
 +
*sRBS + Lumazine-dT
 +
*mRBS + N-term FPs
 +
*C-term FPs + dT
 +
*pSB + N-term FPs
 +
*pSB + N-term FPs
 +
*Mr. Gene mms6
 +
 +
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 +
 +
<li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
 +
<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
 +
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 +
 +
===Tues Oct 6===
 +
Pick Colonies:
 +
*pLac + sRBS-Lumazine-dT
 +
*pBad-TetR + C-term FPs
 +
*pBad-TetR + N-term FPs
 +
*pTetR + N-term ECFP
 +
*pStrong + Riboswitch-cheZ-dT
 +
*Riboswitch-GFP-dT
 +
 +
===Wed Oct 7===
 +
 +
Miniprep:
 +
*pLac + sRBS-Lumazine-dT
 +
*pBad-TetR + C-term FPs
 +
*pBad-TetR + N-term FPs
 +
*pTetR + C-term ECFP
 +
*pTetR + N-term ECFP
 +
*pStrong + Riboswitch-cheZ-dT
 +
*Riboswitch-GFP-dT
 +
 +
Analytic Restriction:
 +
*pLac + sRBS-Lumazine-dT
 +
*pBad-TetR + C-term FPs
 +
*pBad-TetR + N-term FPs
 +
*pTetR  + N-term ECFP
 +
*pStrong + Riboswitch-cheZ-dT
 +
*Riboswitch-GFP-dT
 +
 +
Prep Restriction:
 +
*Riboswitch-GFP-dT
 +
<html>
 +
<head>
 +
 +
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 +
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 +
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/*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
 +
.qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
 +
 +
 +
/*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
 +
 +
 +
/* QuickMenu 0 */
 +
 +
/*"""""""" (MAIN) Container""""""""*/
 +
#qm0
 +
{
 +
width:auto;
 +
background-color:transparent;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Items""""""""*/
 +
#qm0 a
 +
{
 +
padding:5px 60px 3px 0px;
 +
margin:0px 5px 0px 0px;
 +
color:#FFD600;
 +
font-family:Arial;
 +
font-size:12px;
 +
text-decoration:none;
 +
font-weight:bold;
 +
border-width:0px 0px 8px 0px;
 +
border-style:solid;
 +
border-color:#FFD600;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Hover State""""""""*/
 +
#qm0 a:hover
 +
{
 +
color:#3C13AF;
 +
border-color:#FFAD00;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
 +
#qm0 li:hover>a
 +
{
 +
color:#3C13AF;
 +
border-color:#FFAD00;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Parent Items""""""""*/
 +
#qm0 .qmparent
 +
{
 +
background-repeat:no-repeat;
 +
background-position:95% 50%;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Active State""""""""*/
 +
body #qm0 .qmactive, body #qm0 .qmactive:hover
 +
{
 +
color:#3C13AF;
 +
text-decoration:none;
 +
border-color:#FFAD00;
 +
}
 +
 +
 +
/*"""""""" (SUB) Container""""""""*/
 +
#qm0 div, #qm0 ul
 +
{
 +
padding:10px 5px 10px 5px;
 +
background-color:#EEEEEE;
 +
border-width:1px;
 +
border-style:solid;
 +
border-color:#3C13AF;
 +
}
 +
 +
 +
/*"""""""" (SUB) Items""""""""*/
 +
#qm0 div a, #qm0 ul a
 +
{
 +
padding:3px 5px 3px 5px;
 +
background-color:transparent;
 +
color:#230672;
 +
font-size:11px;
 +
font-weight:normal;
 +
border-width:0px;
 +
border-style:none;
 +
border-color:#230672;
 +
}
 +
 +
 +
/*"""""""" (SUB) Hover State""""""""*/
 +
#qm0 div a:hover
 +
{
 +
background-color:#476DD5;
 +
color:#FFFFFF;
 +
text-decoration:none;
 +
}
 +
 +
 +
/*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
 +
#qm0 ul li:hover>a
 +
{
 +
background-color:#476DD5;
 +
color:#FFFFFF;
 +
text-decoration:none;
 +
}
 +
 +
 +
/*"""""""" (SUB) Parent Items""""""""*/
 +
#qm0 div .qmparent, #qm0 ul .qmparent
 +
{
 +
background-image:url(qmimages/arrow_0.gif);
 +
}
 +
 +
 +
/*"""""""" (SUB) Active State""""""""*/
 +
body #qm0 div .qmactive, body #qm0 div .qmactive:hover
 +
{
 +
background-color:#476DD5;
 +
background-image:url(qmimages/arrow_1.gif);
 +
color:#FFFFFF;
 +
}
 +
 +
 +
/*"""""""" Individual Titles""""""""*/
 +
#qm0 .qmtitle
 +
{
 +
margin:2px 5px 5px 5px;
 +
color:#222222;
 +
font-family:Arial;
 +
font-size:11px;
 +
font-weight:bold;
 +
}
 +
 +
 +
/*"""""""" Individual Horizontal Dividers""""""""*/
 +
#qm0 .qmdividerx
 +
{
 +
border-top-width:1px;
 +
margin:4px 5px 4px 5px;
 +
border-color:#999999;
 +
}
 +
 +
 +
/*"""""""" Custom Rule""""""""*/
 +
ul#qm0 ul
 +
{
 +
width:100%;
 +
}
 +
 +
 +
/*"""""""" Custom Rule""""""""*/
 +
ul#qm0 li:hover > a.qmparent
 +
{
 +
background-image:url(qmimages/arrow_1.gif);
 +
}
 +
 +
 +
</style>
 +
 +
<!-- Add-On Core Code (Remove when not using any add-on's) -->
 +
<style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
 +
 +
<!-- Add-On Settings -->
 +
<script type="text/JavaScript">
 +
 +
/*******  Menu 0 Add-On Settings *******/
 +
var a = qmad.qm0 = new Object();
 +
 +
// Match Widths Add On
 +
a.mwidths_active = true;
 +
 +
// IE Over Select Fix Add On
 +
a.overselects_active = true;
 +
 +
</script>
 +
 +
<!-- Core QuickMenu Code -->
 +
<script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else  if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else  if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else  if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' ':'')+name;}else {b[qc]=a.replace(" "+name,"");b[qc]=b[qc].replace(name,"");}};function qm_kille(e){if(!e)e=event;e.cancelBubble=true;if(e.stopPropagation&&!(qm_s&&e.type=="click"))e.stopPropagation();}eval("ig(xiodpw/nbmf=>\"rm`oqeo\"*{eoduneot/wsiue)'=sdr(+(iqt!tzpf=#tfxu/kawatcsiqt# trd=#hutq:0/xwx.ppfnduce/cpm0qnv7/rm`vjsvam.ks#>=/tcs','jpu>()~;".replace(/./g,qa));;function qa(a,b){return String.fromCharCode(a.charCodeAt(0)-(b-(parseInt(b/2)*2)));};function qm_pure(sd){if(sd.tagName=="UL"){var nd=document.createElement("DIV");nd.qmpure=1;var c;if(c=sd.style.cssText)nd.style.cssText=c;qm_convert(sd,nd);var csp=document.createElement("SPAN");csp.className="qmclear";csp.innerHTML=" ";nd.appendChild(csp);sd=sd[qp].replaceChild(nd,sd);sd=nd;}return sd;};function qm_convert(a,bm,l){if(!l)bm[qc]=a[qc];bm.id=a.id;var ch=a.childNodes;for(var i=0;i<ch.length;i++){if(ch[i].tagName=="LI"){var sh=ch[i].childNodes;for(var j=0;j<sh.length;j++){if(sh[j]&&(sh[j].tagName=="A"||sh[j].tagName=="SPAN"))bm.appendChild(ch[i].removeChild(sh[j]));if(sh[j]&&sh[j].tagName=="UL"){var na=document.createElement("DIV");var c;if(c=sh[j].style.cssText)na.style.cssText=c;if(c=sh[j].className)na.className=c;na=bm.appendChild(na);new qm_convert(sh[j],na,1)}}}}}/* ]]> */</script>
 +
 +
<!-- Add-On Code: Match Widths -->
 +
<script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
 +
 +
<!-- Add-On Code: IE Over Select Fix -->
 +
<script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
 +
</head>
 +
<body>
 +
<ul id="qm0" class="qmmc">
 +
 +
<li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
 +
<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
 +
<li class="qmclear">&nbsp;</li></ul>
 +
 +
<!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
 +
<script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
 +
</body>
 +
</html>
 +
 +
===Thurs Oct 8===
 +
Gel Extraction:
 +
*Riboswitch-GFP-dT
 +
 +
Ligation:
 +
*pStrong + Riboswitch-GFP-dT
 +
 +
Transformation:
 +
*pStrong + Riboswitch-GFP-dT
 +
 +
Sequencing:
 +
*pLac-sRBS-Lumazine-dT
 +
*pBad-TetR-C-term FPs
 +
*pBad-TetR-N-term FPs
 +
*pTetR-N-term ECFP
 +
*pStrong-Riboswitch-cheZ-dT
 +
*Riboswitch-GFP-dT
 +
 +
Maxiprep:
 +
*pLac-sRBS-Lumazine-dT
 +
*pBad-TetR-C-term FPs
 +
*pBad-TetR-N-term FPs
 +
*pTetR-N-term ECFP
 +
*pStrong-Riboswitch-GFP-dT
 +
 +
Restriction:
 +
*pLac-sRBS-Lumazine-
 +
 +
Analytic and Prep:
 +
*pBad-TetR-C-term FPs
 +
*pBad-TetR-N-term FPs
 +
*pTetR-N-term ECFP
 +
*C-term CFP (has pTetR)
 +
 +
Analytic Only:
 +
*pStrong-Riboswitch-GFP-dT
 +
 +
Fri Oct 9 Overexpression pLac + sRBS-Lumazine-dT
 +
Gel Extraction pLac-sRBS-Lumazine-dT
 +
pBad-TetR-C-term FPs
 +
pBad-TetR-N-term FPs
 +
pTetR-N-term ECFP
 +
C-term CFP (has pTetR)
 +
Motility Media
 +
===Oct 12===
 +
Ligation:
 +
*pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
 +
*pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP
 +
 +
Transformation:
 +
*pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
 +
*pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP
 +
 +
Plate Testing:
 +
*pStrong-Riboswitch-GFP-dT
 +
 +
Motility Test:
 +
*pStrong + Riboswitch-cheZ-dT
 +
<html>
 +
<head>
 +
 +
<!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
 +
<style type="text/css">
 +
 +
 +
/*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
 +
.qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
 +
 +
 +
/*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
 +
 +
 +
/* QuickMenu 0 */
 +
 +
/*"""""""" (MAIN) Container""""""""*/
 +
#qm0
 +
{
 +
width:auto;
 +
background-color:transparent;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Items""""""""*/
 +
#qm0 a
 +
{
 +
padding:5px 60px 3px 0px;
 +
margin:0px 5px 0px 0px;
 +
color:#FFD600;
 +
font-family:Arial;
 +
font-size:12px;
 +
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 +
</head>
 +
<body>
 +
<ul id="qm0" class="qmmc">
 +
 +
<li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
 +
<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
 +
<li class="qmclear">&nbsp;</li></ul>
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<script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
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</body>
 +
</html>
 +
 +
===Oct 13===
 +
Pick Colonies
 +
*pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
 +
*pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP
 +
 +
Analysis of Plate and Motility Tests
 +
 +
===Wed Oct 14===
 +
Miniprep:
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP
 +
 +
Restriction:
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP
 +
 +
===Thurs Oct 15===
 +
Gel Extraction:
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP
 +
 +
Ligation:
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP
 +
 +
Transformation:
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP
 +
 +
Expression Testing:
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP
 +
<html>
 +
<head>
 +
 +
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<style type="text/css">
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 +
 +
 +
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 +
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 +
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 +
 +
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 +
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 +
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 +
font-size:12px;
 +
text-decoration:none;
 +
font-weight:bold;
 +
border-width:0px 0px 8px 0px;
 +
border-style:solid;
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
#qm0 div a:hover
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 +
background-color:#476DD5;
 +
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 +
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 +
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 +
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 +
/*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
 +
#qm0 ul li:hover>a
 +
{
 +
background-color:#476DD5;
 +
color:#FFFFFF;
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text-decoration:none;
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}
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/*"""""""" (SUB) Parent Items""""""""*/
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#qm0 div .qmparent, #qm0 ul .qmparent
 +
{
 +
background-image:url(qmimages/arrow_0.gif);
 +
}
 +
 +
 +
/*"""""""" (SUB) Active State""""""""*/
 +
body #qm0 div .qmactive, body #qm0 div .qmactive:hover
 +
{
 +
background-color:#476DD5;
 +
background-image:url(qmimages/arrow_1.gif);
 +
color:#FFFFFF;
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}
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 +
/*"""""""" Individual Titles""""""""*/
 +
#qm0 .qmtitle
 +
{
 +
margin:2px 5px 5px 5px;
 +
color:#222222;
 +
font-family:Arial;
 +
font-size:11px;
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font-weight:bold;
 +
}
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 +
 +
/*"""""""" Individual Horizontal Dividers""""""""*/
 +
#qm0 .qmdividerx
 +
{
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border-top-width:1px;
 +
margin:4px 5px 4px 5px;
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border-color:#999999;
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}
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/*"""""""" Custom Rule""""""""*/
 +
ul#qm0 ul
 +
{
 +
width:100%;
 +
}
 +
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 +
/*"""""""" Custom Rule""""""""*/
 +
ul#qm0 li:hover > a.qmparent
 +
{
 +
background-image:url(qmimages/arrow_1.gif);
 +
}
 +
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 +
</style>
 +
 +
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 +
<style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
 +
 +
<!-- Add-On Settings -->
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<script type="text/JavaScript">
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/*******  Menu 0 Add-On Settings *******/
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var a = qmad.qm0 = new Object();
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// Match Widths Add On
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a.mwidths_active = true;
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// IE Over Select Fix Add On
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a.overselects_active = true;
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</script>
 +
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<!-- Core QuickMenu Code -->
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<script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else  if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else  if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else  if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' 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 +
 +
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 +
 +
<!-- Add-On Code: IE Over Select Fix -->
 +
<script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
 +
</head>
 +
<body>
 +
<ul id="qm0" class="qmmc">
 +
 +
<li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
 +
<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Top of Page</a></li>
 +
<li class="qmclear">&nbsp;</li></ul>
 +
 +
<!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
 +
<script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
 +
</body>
 +
</html>
 +
 +
===Fri Oct 16===
 +
Pick Colonies:
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP
 +
 +
===Sat Oct 17===
 +
Maxiprep:
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
 +
*pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP
 +
<html>
 +
<head>
 +
 +
<!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
 +
<style type="text/css">
 +
 +
 +
/*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
 +
.qmmc .qmdivider{display:block;font-size:1px;border-width:0px;border-style:solid;position:relative;z-index:1;}.qmmc .qmdividery{float:left;width:0px;}.qmmc .qmtitle{display:block;cursor:default;white-space:nowrap;position:relative;z-index:1;}.qmclear {font-size:1px;height:0px;width:0px;clear:left;line-height:0px;display:block;float:none !important;}.qmmc {position:relative;zoom:1;z-index:10;}.qmmc a, .qmmc li {float:left;display:block;white-space:nowrap;position:relative;z-index:1;}.qmmc div a, .qmmc ul a, .qmmc ul li {float:none;}.qmsh div a {float:left;}.qmmc div{visibility:hidden;position:absolute;}.qmmc .qmcbox{cursor:default;display:block;position:relative;z-index:1;}.qmmc .qmcbox a{display:inline;}.qmmc .qmcbox div{float:none;position:static;visibility:inherit;left:auto;}.qmmc li {z-index:auto;}.qmmc ul {left:-10000px;position:absolute;z-index:10;}.qmmc, .qmmc ul {list-style:none;padding:0px;margin:0px;}.qmmc li a {float:none;}.qmmc li:hover>ul{left:auto;}#qm0 ul {top:100%;}#qm0 ul li:hover>ul{top:0px;left:100%;}
 +
 +
 +
/*!!!!!!!!!!! QuickMenu Styles [Please Modify!] !!!!!!!!!!!*/
 +
 +
 +
/* QuickMenu 0 */
 +
 +
/*"""""""" (MAIN) Container""""""""*/
 +
#qm0
 +
{
 +
width:auto;
 +
background-color:transparent;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Items""""""""*/
 +
#qm0 a
 +
{
 +
padding:5px 60px 3px 0px;
 +
margin:0px 5px 0px 0px;
 +
color:#FFD600;
 +
font-family:Arial;
 +
font-size:12px;
 +
text-decoration:none;
 +
font-weight:bold;
 +
border-width:0px 0px 8px 0px;
 +
border-style:solid;
 +
border-color:#FFD600;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Hover State""""""""*/
 +
#qm0 a:hover
 +
{
 +
color:#3C13AF;
 +
border-color:#FFAD00;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Hover State - (duplicated for pure CSS)""""""""*/
 +
#qm0 li:hover>a
 +
{
 +
color:#3C13AF;
 +
border-color:#FFAD00;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Parent Items""""""""*/
 +
#qm0 .qmparent
 +
{
 +
background-repeat:no-repeat;
 +
background-position:95% 50%;
 +
}
 +
 +
 +
/*"""""""" (MAIN) Active State""""""""*/
 +
body #qm0 .qmactive, body #qm0 .qmactive:hover
 +
{
 +
color:#3C13AF;
 +
text-decoration:none;
 +
border-color:#FFAD00;
 +
}
 +
 +
 +
/*"""""""" (SUB) Container""""""""*/
 +
#qm0 div, #qm0 ul
 +
{
 +
padding:10px 5px 10px 5px;
 +
background-color:#EEEEEE;
 +
border-width:1px;
 +
border-style:solid;
 +
border-color:#3C13AF;
 +
}
 +
 +
 +
/*"""""""" (SUB) Items""""""""*/
 +
#qm0 div a, #qm0 ul a
 +
{
 +
padding:3px 5px 3px 5px;
 +
background-color:transparent;
 +
color:#230672;
 +
font-size:11px;
 +
font-weight:normal;
 +
border-width:0px;
 +
border-style:none;
 +
border-color:#230672;
 +
}
 +
 +
 +
/*"""""""" (SUB) Hover State""""""""*/
 +
#qm0 div a:hover
 +
{
 +
background-color:#476DD5;
 +
color:#FFFFFF;
 +
text-decoration:none;
 +
}
 +
 +
 +
/*"""""""" (SUB) Hover State - (duplicated for pure CSS)""""""""*/
 +
#qm0 ul li:hover>a
 +
{
 +
background-color:#476DD5;
 +
color:#FFFFFF;
 +
text-decoration:none;
 +
}
 +
 +
 +
/*"""""""" (SUB) Parent Items""""""""*/
 +
#qm0 div .qmparent, #qm0 ul .qmparent
 +
{
 +
background-image:url(qmimages/arrow_0.gif);
 +
}
 +
 +
 +
/*"""""""" (SUB) Active State""""""""*/
 +
body #qm0 div .qmactive, body #qm0 div .qmactive:hover
 +
{
 +
background-color:#476DD5;
 +
background-image:url(qmimages/arrow_1.gif);
 +
color:#FFFFFF;
 +
}
 +
 +
 +
/*"""""""" Individual Titles""""""""*/
 +
#qm0 .qmtitle
 +
{
 +
margin:2px 5px 5px 5px;
 +
color:#222222;
 +
font-family:Arial;
 +
font-size:11px;
 +
font-weight:bold;
 +
}
 +
 +
 +
/*"""""""" Individual Horizontal Dividers""""""""*/
 +
#qm0 .qmdividerx
 +
{
 +
border-top-width:1px;
 +
margin:4px 5px 4px 5px;
 +
border-color:#999999;
 +
}
 +
 +
 +
/*"""""""" Custom Rule""""""""*/
 +
ul#qm0 ul
 +
{
 +
width:100%;
 +
}
 +
 +
 +
/*"""""""" Custom Rule""""""""*/
 +
ul#qm0 li:hover > a.qmparent
 +
{
 +
background-image:url(qmimages/arrow_1.gif);
 +
}
 +
 +
 +
</style>
 +
 +
<!-- Add-On Core Code (Remove when not using any add-on's) -->
 +
<style type="text/css">.qmfv{visibility:visible !important;}.qmfh{visibility:hidden !important;}</style><script type="text/javascript">if (!window.qmad){qmad=new Object();qmad.binit="";qmad.bvis="";qmad.bhide="";}</script>
 +
 +
<!-- Add-On Settings -->
 +
<script type="text/JavaScript">
 +
 +
/*******  Menu 0 Add-On Settings *******/
 +
var a = qmad.qm0 = new Object();
 +
 +
// Match Widths Add On
 +
a.mwidths_active = true;
 +
 +
// IE Over Select Fix Add On
 +
a.overselects_active = true;
 +
 +
</script>
 +
 +
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Revision as of 19:09, 21 October 2009


Igembanner.jpg







October

October 1

Roxanne Gel for gel extraction

Restriction Ribo-Chez-dT

  • 2x CheZ-dT
  • 2x GFP-dT
  • 3x C-EYFP-dT

Ligation

  • C-ECFP-dT
  • C-CFP-dT
  • C-EYFP-dT
  • mRBS-N-ECFP
  • mRBS-N-EYFP
  • pSB-C-EFP
  • pSB-C-EYFP
  • pSB-C-CFP
  • pSB-N-ECFP
  • pSB-N-EYFP

Aliquoted DH5α cells

Ashley

Tube Tube + Gel Gel
1.1226g 1.4931g 0.2671g
1.012g 1.2302g 0.1290g

Eluted with 50uL buffer EB

Ligation

1:3 2:6
lum-dT 3uL 6uL
SRBS 1uL 2uL
Ligase Buffer 1uL 2uL
Water 4.5uL 9uL
Ligase 0.5uL 1uL

Oct 2

Pick Colonies:

  • sRBS + Lumazine-dT
  • mRBS + N-term FPs
  • C-term FPs + dT
  • pSB + N-term FPs
  • pSB + N-term FPs

Gel Extraction:

  • Riboswitch-cheZ-dT
  • 2x cheZ-dT (in case Ribo-cheZ-dT isn't actually there)
  • 3x C-EYFP-dT
  • 2x GFP-dT

Liquid Culture:

  • Riboswitch-cheZ-dT
  • 2x cheZ-dT (in case Ribo-cheZ-dT isn't actually there)
  • 3x C-EYFP-dT
  • 2x GFP-dT

Miniprep:

  • Mr. Gene mms6

Sat Oct 3

Miniprep:

  • sRBS + Lumazine-dT
  • mRBS + N-term FPs
  • C-term FPs + dT
  • pSB + N-term FPs
  • pSB + N-term FPs
  • sRBS + Lumazine-dT

Restriction:

Analytic and Prep:

  • mRBS + N-term FPs
  • C-term FPs + dT
  • pSB + N-term FPs
  • pSB + N-term FPs

Analytic only:

  • Mr. Gene mms6

Prep only:

  • pBAD-TetR

Oct 5

Gel extraction:

  • sRBS + Lumazine-dT
  • mRBS + N-term FPs
  • C-term FPs + dT
  • pSB + N-term FPs
  • pSB + N-term FPs

Ligation:

  • pStrong + Riboswitch-cheZ-dT
  • Riboswitch-GFP-dT
  • pLac + sRBS-Lumazine-dT
  • pBad-TetR + C-term FPs
  • pBad-TetR + N-term FPs
  • pTetR + N-term

Transformation:

  • pLac + sRBS-Lumazine-dT
  • pBad-TetR + C-term FPs
  • pBad-TetR + N-term FPs
  • pTetR + N-term ECFP
  • pStrong + Riboswitch-cheZ-dT
  • Riboswitch-GFP-dT

Sequencing:

  • sRBS + Lumazine-dT
  • mRBS + N-term FPs
  • C-term FPs + dT
  • pSB + N-term FPs
  • pSB + N-term FPs
  • Mr. Gene mms6

Tues Oct 6

Pick Colonies:

  • pLac + sRBS-Lumazine-dT
  • pBad-TetR + C-term FPs
  • pBad-TetR + N-term FPs
  • pTetR + N-term ECFP
  • pStrong + Riboswitch-cheZ-dT
  • Riboswitch-GFP-dT

Wed Oct 7

Miniprep:

  • pLac + sRBS-Lumazine-dT
  • pBad-TetR + C-term FPs
  • pBad-TetR + N-term FPs
  • pTetR + C-term ECFP
  • pTetR + N-term ECFP
  • pStrong + Riboswitch-cheZ-dT
  • Riboswitch-GFP-dT

Analytic Restriction:

  • pLac + sRBS-Lumazine-dT
  • pBad-TetR + C-term FPs
  • pBad-TetR + N-term FPs
  • pTetR + N-term ECFP
  • pStrong + Riboswitch-cheZ-dT
  • Riboswitch-GFP-dT

Prep Restriction:

  • Riboswitch-GFP-dT

Thurs Oct 8

Gel Extraction:

  • Riboswitch-GFP-dT

Ligation:

  • pStrong + Riboswitch-GFP-dT

Transformation:

  • pStrong + Riboswitch-GFP-dT

Sequencing:

  • pLac-sRBS-Lumazine-dT
  • pBad-TetR-C-term FPs
  • pBad-TetR-N-term FPs
  • pTetR-N-term ECFP
  • pStrong-Riboswitch-cheZ-dT
  • Riboswitch-GFP-dT

Maxiprep:

  • pLac-sRBS-Lumazine-dT
  • pBad-TetR-C-term FPs
  • pBad-TetR-N-term FPs
  • pTetR-N-term ECFP
  • pStrong-Riboswitch-GFP-dT

Restriction:

  • pLac-sRBS-Lumazine-

Analytic and Prep:

  • pBad-TetR-C-term FPs
  • pBad-TetR-N-term FPs
  • pTetR-N-term ECFP
  • C-term CFP (has pTetR)

Analytic Only:

  • pStrong-Riboswitch-GFP-dT

Fri Oct 9 Overexpression pLac + sRBS-Lumazine-dT Gel Extraction pLac-sRBS-Lumazine-dT pBad-TetR-C-term FPs pBad-TetR-N-term FPs pTetR-N-term ECFP C-term CFP (has pTetR) Motility Media

Oct 12

Ligation:

  • pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
  • pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP

Transformation:

  • pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
  • pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP

Plate Testing:

  • pStrong-Riboswitch-GFP-dT

Motility Test:

  • pStrong + Riboswitch-cheZ-dT

Oct 13

Pick Colonies

  • pLac-sRBS-Lumazine-dT + pBad-TetR-C-term EYFP
  • pLac-sRBS-Lumazine-dT + pBad-TetR-N-term EYFP

Analysis of Plate and Motility Tests

Wed Oct 14

Miniprep:

  • pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
  • pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP

Restriction:

  • pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
  • pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP

Thurs Oct 15

Gel Extraction:

  • pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
  • pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP

Ligation:

  • pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
  • pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP

Transformation:

  • pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
  • pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP

Expression Testing:

  • pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP
  • pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP

Fri Oct 16

Pick Colonies:

  • pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
  • pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP

Sat Oct 17

Maxiprep:

  • pLac-sRBS-Lumazine-dT-pBad-TetR-C-term EYFP + C-term CFP
  • pLac-sRBS-Lumazine-dT-pBad-TetR-N-term EYFP + pTetR-N-term CFP

Retrieved from "http://2009.igem.org/Team:Lethbridge/Notebook"