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Team:Kyoto/CiC/Parts
From 2009.igem.org
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===Signal for TIM23 complex::EGFP expressing vector for HeLa cells=== | ===Signal for TIM23 complex::EGFP expressing vector for HeLa cells=== | ||
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Revision as of 20:05, 21 October 2009
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Parts
Part design
In this project, we designed two plasmid for RTS; 1. HIV-TAT::(LALAAAA)3 expressing vector 2. Signal for TIM23 complex::GFP expressing vector 3.Signal for TIM23 complex::EGFP expressing vector for HeLa cells. Both of 1 and 3 have T7 promoter, terminator and each CDS between them. They can express each protein by RTS (Rapidly Translation System, Roache). Both parts are inserted in high copy number plasmid pSB1A2. 3.Signal for TIM23 complex::EGFP expressing vector for HeLa cells is made by inserting Signal for TIM23 complex into pEGFP-N3.
HIV-TAT-(LALAAAA)3-generator
TIM23complex signal::EGFP generator
Signal for TIM23 complex::EGFP expressing vector for HeLa cells
2.png)
