Team:Imperial College London/Major results
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*The wet lab has also investigated the [[Team:Imperial_College_London/Wetlab/Results/RestrictionDigest | effects of restriction enzymes on cell population]]. Positive results were achieved. | *The wet lab has also investigated the [[Team:Imperial_College_London/Wetlab/Results/RestrictionDigest | effects of restriction enzymes on cell population]]. Positive results were achieved. | ||
- | [[Image:II09_DpnII_Digest.png |400px][[Image:II09_TaqI_Digest.png | 400px]] | + | [[Image:II09_DpnII_Digest.png |400px]][[Image:II09_TaqI_Digest.png | 400px]] |
The Dam negative strains are fully cleaved at all different concentrations of restriction enzyme. This produces the 'smear' that can be seen, as the DNA fragments are all of varying, but short lengths | The Dam negative strains are fully cleaved at all different concentrations of restriction enzyme. This produces the 'smear' that can be seen, as the DNA fragments are all of varying, but short lengths |
Revision as of 21:01, 21 October 2009
Contents |
Major Results
This page contains highlights of our major results and simulations. It is by no means complete. However, more results will be presented at the jamboree.
Module 2 - Encapsulation
Wet lab experiments were performed to investigate growth of E. Coli cells under environments of different pH.
Module 3 - Genome Deletion
- The wet lab has also investigated the effects of restriction enzymes on cell population. Positive results were achieved.
The Dam negative strains are fully cleaved at all different concentrations of restriction enzyme. This produces the 'smear' that can be seen, as the DNA fragments are all of varying, but short lengths
From these results, we have shown that restriction enzymes can cleave Dam negative strains, hence killing Dam negative cells. This also proves Dam positive strains are protected again genomic deletion.
cell death model
Module Integration - Autoinduction
crp promoter
diauxic growth
Module Integration - Thermoinduction
hvd gfp
References
[1]Physica A: Statistical and Theoretical Physics, Vol. 188, No. 1-3. (1 September 1992), pp. 404-425. A rigorous derivation of the chemical master Equation